Hexidium Iodide CAS 211566-66-4

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Physical properties

Molecular weight	497.41
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

CAS

211566-66-4

Molecular weight

497.41

The nucleic acid stain, hexidium iodide is permeant to mammalian cells and selectively stains almost all gram-positive bacteria in the presence of gram-negative bacteria. Hexidium iodide exhibits excitation/emission maxima of ~518/600 nm upon binding DNA. Generally, both the cytoplasm and nuclei of eukaryotic cells show staining with hexidium iodide. We do not recommend its use as a nuclear stain since it also stains other subcelluar compartments.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Hexidium Iodide *CAS 211566-66-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.041 µL	1.005 mL	2.01 mL	10.052 mL	20.104 mL
5 mM	40.208 µL	201.041 µL	402.083 µL	2.01 mL	4.021 mL
10 mM	20.104 µL	100.521 µL	201.041 µL	1.005 mL	2.01 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield
Propidium iodide CAS 25535-16-4	537	618	6000¹	0.2¹
Propidium iodide 10 mM aqueous solution	537	618	6000¹	0.2¹
DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]	754	778	270000¹	-
DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide]	550	564	148000	-
Propidium iodide 1 mg/mL aqueous solution	537	618	6000¹	0.2¹

Images

Figure 1. Chemical structure for Hexidium Iodide *CAS 211566-66-4*

Citations

View all 1 citations: Citation Explorer

Functional and Quantitative High-Throughput Screening for Hydrolases Secreted by B. subtilis
Authors: Dabene, Valentina
Journal: (2021)

References

View all 13 references: Citation Explorer

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Journal: Int J Food Microbiol (2003): 269

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Journal: J Microbiol Methods (2003): 193

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Electrophoretic behavior of individual nuclear species as determined by capillary electrophoresis with laser-induced fluorescence detection
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Journal: Electrophoresis (2002): 2110

Antigene effect in K562 cells of a PEG-conjugated triplex-forming oligonucleotide targeted to the bcr/abl oncogene
Authors: Rapozzi V, Cogoi S, Spessotto P, Risso A, Bonora GM, Quadrifoglio F, Xodo LE.
Journal: Biochemistry (2002): 502

Simultaneous fluorescent gram staining and activity assessment of activated sludge bacteria
Authors: Forster S, Snape JR, Lappin-Scott HM, Porter J.
Journal: Appl Environ Microbiol (2002): 4772