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Hexidium Iodide *CAS 211566-66-4*

The nucleic acid stain, hexidium iodide is permeant to mammalian cells and selectively stains almost all gram-positive bacteria in the presence of gram-negative bacteria. Hexidium iodide exhibits excitation/emission maxima of ~518/600 nm upon binding DNA. Generally, both the cytoplasm and nuclei of eukaryotic cells show staining with hexidium iodide. We do not recommend its use as a nuclear stain since it also stains other subcelluar compartments.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Hexidium Iodide *CAS 211566-66-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.041 µL1.005 mL2.01 mL10.052 mL20.104 mL
5 mM40.208 µL201.041 µL402.083 µL2.01 mL4.021 mL
10 mM20.104 µL100.521 µL201.041 µL1.005 mL2.01 mL

Molarity calculator

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References

View all 13 references: Citation Explorer
Novel model for multispecies biofilms that uses rigid gas-permeable lenses
Authors: Peyyala R, Kirakodu SS, Ebersole JL, Novak KF.
Journal: Appl Environ Microbiol (2011): 3413
Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum
Authors: Linhova M, Patakova P, Lipovsky J, Fribert P, Paulova L, Rychtera M, Melzoch K.
Journal: Folia Microbiol (Praha) (2010): 340
A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk
Authors: Holm C, Mathiasen T, Jespersen L.
Journal: J Appl Microbiol (2004): 935
Metabolic activity in filamentous fungi can be analysed by flow cytometry
Authors: Bradner JR, Nevalainen KM.
Journal: J Microbiol Methods (2003): 193
Potential for broad applications of flow cytometry and fluorescence techniques in microbiological and somatic cell analyses of milk
Authors: Gunasekera TS, Veal DA, Attfield PV.
Journal: Int J Food Microbiol (2003): 269
Page updated on October 12, 2024

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Physical properties

Molecular weight

497.41

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134

CAS

211566-66-4
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