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Hexidium Iodide *CAS 211566-66-4*

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Physical properties
Molecular weight497.41
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
The nucleic acid stain, hexidium iodide is permeant to mammalian cells and selectively stains almost all gram-positive bacteria in the presence of gram-negative bacteria. Hexidium iodide exhibits excitation/emission maxima of ~518/600 nm upon binding DNA. Generally, both the cytoplasm and nuclei of eukaryotic cells show staining with hexidium iodide. We do not recommend its use as a nuclear stain since it also stains other subcelluar compartments.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Hexidium Iodide *CAS 211566-66-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.041 µL1.005 mL2.01 mL10.052 mL20.104 mL
5 mM40.208 µL201.041 µL402.083 µL2.01 mL4.021 mL
10 mM20.104 µL100.521 µL201.041 µL1.005 mL2.01 mL

Molarity calculator

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View all 13 references: Citation Explorer
Novel model for multispecies biofilms that uses rigid gas-permeable lenses
Authors: Peyyala R, Kirakodu SS, Ebersole JL, Novak KF.
Journal: Appl Environ Microbiol (2011): 3413
Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum
Authors: Linhova M, Patakova P, Lipovsky J, Fribert P, Paulova L, Rychtera M, Melzoch K.
Journal: Folia Microbiol (Praha) (2010): 340
A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk
Authors: Holm C, Mathiasen T, Jespersen L.
Journal: J Appl Microbiol (2004): 935
A flow-cytometric gram-staining technique for milk-associated bacteria
Authors: Holm C, Jespersen L.
Journal: Appl Environ Microbiol (2003): 2857
Potential for broad applications of flow cytometry and fluorescence techniques in microbiological and somatic cell analyses of milk
Authors: Gunasekera TS, Veal DA, Attfield PV.
Journal: Int J Food Microbiol (2003): 269
Metabolic activity in filamentous fungi can be analysed by flow cytometry
Authors: Bradner JR, Nevalainen KM.
Journal: J Microbiol Methods (2003): 193
Antiproliferative effect in chronic myeloid leukaemia cells by antisense peptide nucleic acids
Authors: Rapozzi V, Burm BE, Cogoi S, van der Marel GA, van Boom JH, Quadrifoglio F, Xodo LE.
Journal: Nucleic Acids Res (2002): 3712
Electrophoretic behavior of individual nuclear species as determined by capillary electrophoresis with laser-induced fluorescence detection
Authors: Gunasekera N, Musier-Forsyth K, Arriaga E.
Journal: Electrophoresis (2002): 2110
Antigene effect in K562 cells of a PEG-conjugated triplex-forming oligonucleotide targeted to the bcr/abl oncogene
Authors: Rapozzi V, Cogoi S, Spessotto P, Risso A, Bonora GM, Quadrifoglio F, Xodo LE.
Journal: Biochemistry (2002): 502
Simultaneous fluorescent gram staining and activity assessment of activated sludge bacteria
Authors: Forster S, Snape JR, Lappin-Scott HM, Porter J.
Journal: Appl Environ Microbiol (2002): 4772