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LysoBrite™ Green DND-26

Images of HeLa cells stained with LysoBrite™ Green DND-26 (Cat No. 22648) in a costar black wall/clear bottom 96-well plate. The cells were imaged using a fluorescence microscope with a FITC filter set.
Images of HeLa cells stained with LysoBrite™ Green DND-26 (Cat No. 22648) in a costar black wall/clear bottom 96-well plate. The cells were imaged using a fluorescence microscope with a FITC filter set.
Images of HeLa cells stained with LysoBrite™ Green DND-26 (Cat No. 22648) in a costar black wall/clear bottom 96-well plate. The cells were imaged using a fluorescence microscope with a FITC filter set.
Gallery Image 2
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties
Molecular weight362.23
SolventDMSO
Spectral properties
Excitation (nm)501
Emission (nm)509
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Lysosomes
Molecular weight
362.23
Excitation (nm)
501
Emission (nm)
509
LysoBrite™ Green DND-26 is chemically the same as the LysoTracker® Green DND-26 used for labeling and tracking acidic organelles in live cells (LysoTracker® is the trademark of ThermoFisher). It has good selectivity for acidic organelles. The LysoBrite™ probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH, allowing them to freely permeate cell membranes to label live cells.

Platform


Flow cytometer

Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Green DND-26 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM276.068 µL1.38 mL2.761 mL13.803 mL27.607 mL
5 mM55.214 µL276.068 µL552.135 µL2.761 mL5.521 mL
10 mM27.607 µL138.034 µL276.068 µL1.38 mL2.761 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)501
Emission (nm)509

Images


Citations


View all 1 citations: Citation Explorer
Two-Pronged Intracellular Co-Delivery of Antigen and Adjuvant for Synergistic Cancer Immunotherapy
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168

References


View all 50 references: Citation Explorer
Synergistic effects of botanical curcumin-induced programmed cell death on the management of Spodoptera litura Fabricius with avermectin.
Authors: Cui, Gaofeng and Yuan, Haiqi and He, Wei and Deng, Yukun and Sun, Ranran and Zhong, Guohua
Journal: Ecotoxicology and environmental safety (2022): 113097
Presenilin/γ-Secretase Activity Is Located in Acidic Compartments of Live Neurons.
Authors: Maesako, Masato and Houser, Mei C Q and Turchyna, Yuliia and Wolfe, Michael S and Berezovska, Oksana
Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (2022): 145-154
Structural Modifications to the Internal Oligoguanidinium Transporter Uncover Two Potent Analogues that Effectively Deliver the Proapoptotic Peptide in Multiple Cancer Cell Lines.
Authors: Gupta, Shalini and Qasim, Md and Gupta, Abhishek and Kundu, Jayanta and Sinha, Surajit
Journal: Bioconjugate chemistry (2022): 121-133
Incidence of an Intracellular Multiplication Niche among Acinetobacter baumannii Clinical Isolates.
Authors: Rubio, Tristan and Gagné, Stéphanie and Debruyne, Charline and Dias, Chloé and Cluzel, Caroline and Mongellaz, Doriane and Rousselle, Patricia and Göttig, Stephan and Seifert, Harald and Higgins, Paul G and Salcedo, Suzana P
Journal: mSystems (2022): e0048821
Zeb1-mediated autophagy enhances resistance of breast cancer cells to genotoxic drugs.
Authors: Fedorova, Olga and Daks, Alexandra and Parfenyev, Sergey and Shuvalov, Oleg and Netsvetay, Sofia and Vasileva, Julia and Gudovich, Anastasia and Golotin, Vasilii and Semenov, Oleg and Petukhov, Alexey and Baiduik, Ekaterina and Berdigaliyev, Nurken and Tulchinsky, Eugene M and Barlev, Nikolai A
Journal: Biochemical and biophysical research communications (2022): 29-34
Raman and fluorescence imaging of phospholipidosis induced by cationic amphiphilic drugs in endothelial cells.
Authors: Bik, Ewelina and Orleanska, Jagoda and Mateuszuk, Lukasz and Baranska, Malgorzata and Majzner, Katarzyna and Chlopicki, Stefan
Journal: Biochimica et biophysica acta. Molecular cell research (2022): 119186
Differential LysoTracker Uptake Defines Two Populations of Distal Epithelial Cells in Idiopathic Pulmonary Fibrosis.
Authors: Wasnick, Roxana Maria and Shalashova, Irina and Wilhelm, Jochen and Khadim, Ali and Schmidt, Nicolai and Hackstein, Holger and Hecker, Andreas and Hoetzenecker, Konrad and Seeger, Werner and Bellusci, Saverio and El Agha, Elie and Ruppert, Clemens and Guenther, Andreas
Journal: Cells (2022)
Quinacrine-Induced Autophagy in Ovarian Cancer Triggers Cathepsin-L Mediated Lysosomal/Mitochondrial Membrane Permeabilization and Cell Death.
Authors: Thirusangu, Prabhu and Pathoulas, Christopher L and Ray, Upasana and Xiao, Yinan and Staub, Julie and Jin, Ling and Khurana, Ashwani and Shridhar, Viji
Journal: Cancers (2021)
Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells.
Authors: Saini, Heena and Sharma, Harshita and Mukherjee, Sudeshna and Chowdhury, Shibasish and Chowdhury, Rajdeep
Journal: Cancer cell international (2021): 52
Human Corneal Epithelial Cells Internalize Aspergillus flavus Spores by Actin-Mediated Endocytosis.
Authors: Arunachalam, Divya and Namperumalsamy, Venkatesh Prajna and Prajna, Lalitha and Kuppamuthu, Dharmalingam
Journal: Infection and immunity (2021)