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LysoBrite™ Green DND-26

Images of HeLa cells stained with LysoBrite™ Green DND-26 (Cat No. 22648) in a costar black wall/clear bottom 96-well plate. The cells were imaged using a fluorescence microscope with a FITC filter set.
Images of HeLa cells stained with LysoBrite™ Green DND-26 (Cat No. 22648) in a costar black wall/clear bottom 96-well plate. The cells were imaged using a fluorescence microscope with a FITC filter set.
Images of HeLa cells stained with LysoBrite™ Green DND-26 (Cat No. 22648) in a costar black wall/clear bottom 96-well plate. The cells were imaged using a fluorescence microscope with a FITC filter set.
Gallery Image 2
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ShippingStandard overnight for United States, inquire for international
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Physical properties
Molecular weight362.23
Spectral properties
Excitation (nm)501
Emission (nm)509
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Lysosomes
Molecular weight
Excitation (nm)
Emission (nm)
LysoBrite™ Green DND-26 is chemically the same as the LysoTracker® Green DND-26 used for labeling and tracking acidic organelles in live cells (LysoTracker® is the trademark of ThermoFisher). It has good selectivity for acidic organelles. The LysoBrite™ probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH, allowing them to freely permeate cell membranes to label live cells.


Flow cytometer

Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Green DND-26 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM276.068 µL1.38 mL2.761 mL13.803 mL27.607 mL
5 mM55.214 µL276.068 µL552.135 µL2.761 mL5.521 mL
10 mM27.607 µL138.034 µL276.068 µL1.38 mL2.761 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


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Spectral properties

Excitation (nm)501
Emission (nm)509



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