Name: LysoBrite™ Blue
Brand: AAT Bioquest
SKU: 22642
Price: 174 USD
Availability: InStock

LysoBrite™ Blue

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Blue selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Catalog	Size	Price	Quantity
22642	500 Tests	Price

Citations

View all 11 citations: Citation Explorer

Enhancing Anti-Tumor Effects of Engineered Extracellular Vesicles via Endocytosis Route Switching and Interferon Response Suppression

Authors:

Huang, Zixuan and Lu, Chaoqun and Wang, Yixin and Xian, Huajian and Zheng, Yuling and Kang, Ting and Xiang, Rufang and Xie, Shufeng and Wang, Minghui and Li, Zeyi and others,

Journal:

Advanced Science (2025): e15472

Magnetic Field-Induced Drug Delivery from ZIF-8 Metal-Organic Frameworks-Based Nanocomposites through Magnetic Nanoparticles Heating or Motion

Authors:

Abdelhamid, Ahmed and Alvarez, Aitor and Laib, Loubna and Clerc, Pascal and Ceballos, Manuel and Journaux, Justine and Misra, Anil and Alavijeh, Mohammad S and Ballon, G{\'e}raldine and Bousquet, Corinne and others,

Identification of natural compound garcinone E as a novel autophagic flux inhibitor with anticancer effect in nasopharyngeal carcinoma cells

Authors:

Wei, Dan and Wang, Luolin and Lei, Shunmei and Zhang, Han and Dong, Caihua and Ke, Yao and Su, Yuting and Chen, Xiaoying and Xia, Lianping and Kong, Xiaoyang and others,

Journal:

Pharmaceutical Biology (2023): 839--857

LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection

Authors:

Fan, Shuangqi and Wu, Keke and Zhao, Mingqiu and Yuan, Jin and Ma, Shengming and Zhu, Erpeng and Chen, Yuming and Ding, Hongxing and Yi, Lin and Chen, Jinding

Journal:

Autophagy (2020): 1--20

Apatite-Binding Nanoparticulate Agonist of Hedgehog Signaling for Bone Repair

Authors:

Zhang, Xiao and Fan, Jiabing and Lee, Chung-Sung and Kim, Soyon and Chen, Chen and Aghaloo, Tara and Lee, Min

Journal:

Advanced Functional Materials (2020): 1909218

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

Evaluation of a high-content screening fluorescence-based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophages

Authors:

Werner T, Liebisch G, Gr and l M, Schmitz G.

Journal:

Cytometry A (2006): 200

Physical properties

Molecular weight	331.42
Solvent	DMSO

Spectral properties

Excitation (nm)	434
Emission (nm)	480

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	450/40 nm filter
Instrument specification(s)	Pacific Blue channel

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

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Fax
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Custom size	Inquire
Technical Support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025