LysoBrite™ Red

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Red selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite™ dyes significantly outperform the equivalent LysoTracker ™dyes (from Invitrogen). LysoBrite™ dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. LysoBrite™ dyes can survive a few generations of cell division. In addition, LysoBrite™ dyes are much more photostable than the LysoTracker dyes.

Protocol

SDS

Catalog	Size	Price	Quantity
22645	500 Tests	Price

Citations

View all 27 citations: Citation Explorer

Primary cilia in growth plates orchestrate long bone development

Authors:

Zhang, Lei and Xu, Xiaoqiao and Tao, Dike and Li, Xinyu and Niu, Pingping and Gong, Xuyan and Li, Gongchen and Yu, Mengfei and Sun, Yao

Journal:

Fundamental Research (2025)

PPAR$\beta$/$\delta$ upregulates the insulin receptor $\beta$ subunit in skeletal muscle by reducing lysosomal activity and EphB4 levels

Authors:

Wang, Jue-Rui and Jurado-Aguilar, Javier and Barroso, Emma and Rodr{\'\i}guez-Calvo, Ricardo and Camins, Antoni and Wahli, Walter and Palomer, Xavier and V{\'a}zquez-Carrera, Manuel

Journal:

Cell Communication and Signaling (2024): 1--12

Assessing the Effects of Surgical Irrigation Solutions on Human Neutrophil Interactions with Nascent Staphylococcus aureus Biofilms

Authors:

Gaur, Gauri and Predtechenskaya, Maria and Voyich, Jovanka M and James, Garth and Stewart, Philip S and Borgogna, Timothy R

Journal:

Microorganisms (2024): 1951

In situ reprogramming of tumor-associated macrophages enhances cancer immunotherapy

Authors:

Han, Yanjie and Lu, Haifeng and Gu, Zikuan and Guan, Peixin and Liu, Zhen

Journal:

Nano Today (2024): 102420

Ferroptosis induces nucleolar stress as revealed by live-cell imaging using thioflavin T

Authors:

Hirata, Yoko and Takemori, Hiroshi and Furuta, Kyoji and Kamatari, Yuji O and Sawada, Makoto

Journal:

Current Research in Pharmacology and Drug Discovery (2024): 100196

References

View all 26 references: Citation Explorer

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

High-content screening: emerging hardware and software technologies

Authors:

Lee S, Howell BJ.

Journal:

Methods Enzymol (2006): 468

Physical properties

Molecular weight	698.94
Solvent	DMSO

Spectral properties

Excitation (nm)	576
Emission (nm)	596

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	532/561 nm laser
Emission	585/40 nm filter

Fluorescence microscope
Excitation	TRITC filter set
Emission	TRITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 26, 2026