mFluor™ Red 780 Maleimide

mFluor™ Red 780 dyes are an excellent alternative to APC-Alexa Fluor® 750 tandems since they have the spectral properties equivalent to those of APC-Alexa Fluor® 750 conjugates. mFluor™ Red 780 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Red 780 dyes are well excited at 633 nm or 647 nm to give red fluorescence (compatible with Cy7® filter). mFluor™ Red 780 dyes and conjugates are excellent red laser reagents for flow cytometry research. Compared to APC-Alexa Fluor® 750 tandems, mFluor™ Red 780 dyes are much more photostable, making them readily available for fluorescence imaging applications while it is very difficult to use the APC-Alexa Fluor® 750 conjugates for fluorescence imaging applications due to the rapid photobleaching of APC-Alexa Fluor® 750 tandems. mFluor™ Red 780 maleimide is stable and highly reacts with thiol-containing biomolecules such as reduced antibodies.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. mFluor Red 780 maleimide readily reacts with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1192	1 mg	Price

References

View all 30 references: Citation Explorer

Creation of a Nanobody-Based Fluorescent Immunosensor Mini Q-body for Rapid Signal-On Detection of Small Hapten Methotrexate.

Authors:

Inoue, Akihito and Ohmuro-Matsuyama, Yuki and Kitaguchi, Tetsuya and Ueda, Hiroshi

Journal:

ACS sensors (2020)

Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine.

Authors:

Jeong, Hee-Jin and Dong, Jinhua and Yeom, Chang-Hun and Ueda, Hiroshi

Journal:

Methods and protocols (2020)

Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging.

Authors:

Alam, Md Kausar and El-Sayed, Ayman and Barreto, Kris and Bernhard, Wendy and Fonge, Humphrey and Geyer, C Ronald

Journal:

Molecular imaging and biology (2019): 54-66

Minimizing the Influence of Fluorescent Tags on IgG Partition in PEG-Salt Aqueous Two-Phase Systems for Rapid Screening Applications.

Authors:

São Pedro, Mariana N and Azevedo, Ana M and Aires-Barros, Maria R and Soares, Ruben R G

Journal:

Biotechnology journal (2019): e1800640

Optimizing leading edge F-actin labeling using multiple actin probes, fixation methods and imaging modalities.

Authors:

DesMarais, Vera and Eddy, Robert J and Sharma, Ved P and Stone, Orrin and Condeelis, John S

Journal:

BioTechniques (2019): 113-119

Physical properties

Molecular weight	1168.28
Solvent	DMSO

Spectral properties

Absorbance (nm)	630
Correction factor (260 nm)	0.101
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	629
Emission (nm)	767

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 22, 2025