Name: mFluor™ UVB 370 SE
Brand: AAT Bioquest
SKU: 70700
Price: 301 USD
Availability: InStock

mFluor™ UVB 370 SE

mFluor™ UVB 370 SE is a UV excitable fluorophore (Ex: 320 nm) with unique spectral properties that expands multicolor flow cytometry into the deep UV range for advanced panel design.

Unique UV Spectral Window: Excitation at ~320 nm and emission at ~370 nm provides a distinct color channel with minimal spectral overlap for advanced multicolor panel design
Optimized for Flow Cytometry: Large Stokes shift and compatibility with standard UV laser lines (320 nm) make it ideal for both conventional and spectral flow cytometry applications
Small Molecule Conjugation: Compact organic dye structure enables efficient antibody labeling with minimal impact on binding specificity or affinity
High Expression Target Compatible: Bright fluorescence output recommended for abundant markers like CD4, maximizing signal detection in multicolor experiments

Protocol

SDS

COA

Catalog	Size	Price	Quantity
70700	1 mg	Price

Overview

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts and can be well excited by the laser lines of flow cytometers (e.g., 355 nm, 405 nm, 488 nm and 633 nm). mFluor™ UVB 370 dyes have fluorescence excitation and emission maxima of ~320 nm and ~370 nm respectively. These spectral characteristics make them a unique color for designing multicolor flow cytometry panels. mFluor™ UVB 370 dyes are well excited by UV laser of 320 nm with emission centered around 370 nm. It is particularly useful for spectral flow cytometry since there are very few existing fluorescent dyes that overlap with their spectra. They are small organic molecules that can be readily conjugated to antibodies. We recommend them to be used for brighter markers (such as CD4).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ UVB 370 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.621 µL	433.103 µL	866.206 µL	4.331 mL	8.662 mL
5 mM	17.324 µL	86.621 µL	173.241 µL	866.206 µL	1.732 mL
10 mM	8.662 µL	43.31 µL	86.621 µL	433.103 µL	866.206 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1154.46 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Water-Soluble Fluorescent Polymer Dyes with Tunable Emission Spectra for Flow Cytometry Applications.

Authors:

Berndt, Daniel and Glaap, Dorina and Jennings, Travis and Dose, Christian and Werz, Daniel B and Reckert, Dirk N H

Journal:

Angewandte Chemie (International ed. in English) (2024): e202402616

High-dimensional multi-pass flow cytometry via spectrally encoded cellular barcoding.

Authors:

Kwok, Sheldon J J and Forward, Sarah and Fahlberg, Marissa D and Assita, Emane Rose and Cosgriff, Sean and Lee, Seung Hyung and Abbott, Geoffrey R and Zhu, Han and Minasian, Nicolas H and Vote, A Sean and Martino, Nicola and Yun, Seok-Hyun

Journal:

Nature biomedical engineering (2024): 310-324

Spectral Flow Cytometry Analysis of Resident Tissue Macrophages.

Authors:

Kong, Wan Ting and Bied, Mathilde and Ginhoux, Florent

Journal:

Methods in molecular biology (Clifton, N.J.) (2024): 269-280

Color-scalable flow cytometry with Raman tags.

Authors:

Nishiyama, Ryo and Hiramatsu, Kotaro and Kawamura, Shintaro and Dodo, Kosuke and Furuya, Kei and de Pablo, Julia Gala and Takizawa, Shigekazu and Min, Wei and Sodeoka, Mikiko and Goda, Keisuke

Journal:

PNAS nexus (2023): pgad001

Development of Spectral Nano-Flow Cytometry for High-Throughput Multiparameter Analysis of Individual Biological Nanoparticles.

Authors:

Li, Lihong and Wang, Shuo and Xue, Junwei and Lin, Yao and Su, Liyun and Xue, Chengfeng and Mao, Cuiping and Cai, Niangui and Tian, Ye and Zhu, Shaobin and Wu, Lina and Yan, Xiaomei

Journal:

Analytical chemistry (2023): 3423-3433

Physical properties

Molecular weight	1154.46
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.395
Extinction coefficient (cm ^-1 M ^-1)	42734 ¹
Excitation (nm)	322
Emission (nm)	368

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 16, 2025