Name: mFluor™ UVB 460 SE
Brand: AAT Bioquest
SKU: 70705
Price: 301 USD
Availability: InStock

mFluor™ UVB 460 SE

mFluor™ UVB 460 SE is an amine-reactive dye optimally excited at 320 nm that provides a distinct blue emission channel for creating multiparameter flow cytometry panels with UV laser systems.

Extended UV-to-Blue Emission: Excitation at ~320 nm with emission at ~460 nm creates an exceptionally large Stokes shift for reduced spectral crosstalk in multicolor panels
Unique Spectral Niche: Fills a rare spectral window with minimal overlap with conventional fluorophores
High Extinction Coefficient: Strong absorption (166,181 cm⁻¹M⁻¹) ensures efficient excitation and bright signal generation with standard UV laser lines
Optimized for Abundant Markers: Small organic dye structure ideal for antibody conjugation, recommended for high-expression targets like CD4

Protocol

SDS

COA

Catalog	Size	Price	Quantity
70705	1 mg	Price

Overview

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts and can be well excited by the laser lines of flow cytometers (e.g., 355 nm, 405 nm, 488 nm and 633 nm). mFluor™ UVB 460 dyes have fluorescence excitation and emission maxima of ~320 nm and ~460 nm respectively. These spectral characteristics make them a unique color for designing multicolor flow cytometry panels. mFluor™ UVB 460 dyes are well excited by UV laser of 320 nm with emission centered around 460 nm. It is particularly useful for spectral flow cytometry since there are very few existing fluorescent dyes that overlap with their spectra. They are small organic molecules that can be readily conjugated to antibodies. We recommend them to be used for brighter markers (such as CD4).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ UVB 460 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	91.46 µL	457.298 µL	914.595 µL	4.573 mL	9.146 mL
5 mM	18.292 µL	91.46 µL	182.919 µL	914.595 µL	1.829 mL
10 mM	9.146 µL	45.73 µL	91.46 µL	457.298 µL	914.595 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1093.38 g/mol	=	mL	x	mM	=	mmol

References

View all 27 references: Citation Explorer

The Glycolysis-derived α-Dicarbonyl Metabolite Methylglyoxal is a UVA-photosensitizer Causing the Photooxidative Elimination of HaCaT Keratinocytes with Induction of Oxidative and Proteotoxic Stress Response Gene Expression†.

Authors:

de Faria Lopes, Lohanna and Jandova, Jana and Justiniano, Rebecca and Perer, Jessica and Baptista, Maurício S and Wondrak, Georg T

Journal:

Photochemistry and photobiology (2023): 826-834

Cell-by-cell estimation of PAH sorption and subsequent toxicity in marine phytoplankton.

Authors:

Kottuparambil, Sreejith and Agusti, Susana

Journal:

Chemosphere (2020): 127487

Deep ultraviolet lasers for flow cytometry.

Authors:

Telford, William and Georges, Thierry and Miller, Clint and Voluer, Pascal

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2019): 227-233

Ultraviolet 320 nm laser excitation for flow cytometry.

Authors:

Telford, William and Stickland, Lynn and Koschorreck, Marco

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 314-325

Near-ultraviolet laser diodes for brilliant ultraviolet fluorophore excitation.

Authors:

Telford, William G

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2015): 1127-37

Physical properties

Molecular weight	1093.38
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.573
Extinction coefficient (cm ^-1 M ^-1)	16618 ¹
Excitation (nm)	325
Emission (nm)	459

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 17, 2025