MycoLight™ Bacterial Viability Assay Kit
AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell. The kit utilizes the mixture of our green fluorescent nucleic acid stain MycoLight™ Green and the red-fluorescent nucleic acid stain propidium iodide. When used alone, the MycoLight™ Green stain generally labels all bacteria (live and dead) in a population. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a reduction in the MycoLight™ Green stain fluorescence when both dyes are present. Thus, with an appropriate mixture of the MycoLight™ Green and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead or dying bacteria with damaged membranes gives red fluorescence. The Mycolight ™ Bacterial Viability Assay Kit is a robust tool for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell. Stained cells can be monitored fluorimetrically at 510-530 nm (FITC filter) and 600-660 nm (Texas red filter) with excitation at 488 nm, the most common excitation light source.
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Citations
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An epigallocatechin gallate--amorphous calcium phosphate nanocomposite for caries prevention and demineralized enamel restoration
Authors: Dai, Danni and Wang, Jianrong and Xie, Hanshu and Zhang, Chao
Journal: Materials Today Bio (2023): 100715
Authors: Dai, Danni and Wang, Jianrong and Xie, Hanshu and Zhang, Chao
Journal: Materials Today Bio (2023): 100715
Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures
Authors: Biagini, Francesco and Calvigioni, Marco and De Maria, Carmelo and Magliaro, Chiara and Montemurro, Francesca and Mazzantini, Diletta and Celandroni, Francesco and Mattioli-Belmonte, Monica and Ghelardi, Emilia and Vozzi, Giovanni
Journal: Bioengineering (2022): 96
Authors: Biagini, Francesco and Calvigioni, Marco and De Maria, Carmelo and Magliaro, Chiara and Montemurro, Francesca and Mazzantini, Diletta and Celandroni, Francesco and Mattioli-Belmonte, Monica and Ghelardi, Emilia and Vozzi, Giovanni
Journal: Bioengineering (2022): 96
Construction of Iron-Scavenging Hydrogel via Thiol--Ene Click Chemistry for Antibiotic-Free Treatment of Bacterial Wound Infection
Authors: Xie, Tian-Qiu and Yan, Xiao and Yan, Jian-Hua and Yu, Yun-Jian and Liu, Xin-Hua and Feng, Jun and Liu, Chuan-Jun and Zhang, Xian-Zheng
Journal: Advanced Healthcare Materials : 2401118
Authors: Xie, Tian-Qiu and Yan, Xiao and Yan, Jian-Hua and Yu, Yun-Jian and Liu, Xin-Hua and Feng, Jun and Liu, Chuan-Jun and Zhang, Xian-Zheng
Journal: Advanced Healthcare Materials : 2401118
Low-friction soft robots for targeted bacterial infection treatment in gastrointestinal tract
Authors: Wang, Ben and Chen, Yunrui and Ye, Zhicheng and Yu, Haidong and Chan, Kai Fung and Xu, Tiantian and Guo, Zhiguang and Liu, Weimin and Zhang, Li
Journal: Cyborg and Bionic Systems
Authors: Wang, Ben and Chen, Yunrui and Ye, Zhicheng and Yu, Haidong and Chan, Kai Fung and Xu, Tiantian and Guo, Zhiguang and Liu, Weimin and Zhang, Li
Journal: Cyborg and Bionic Systems
References
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Authors: Hu, W.; Murata, K.; Zhang, D.
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Authors: Wahman, D. G.; Schrantz, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2010): 8277-80
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Authors: Wahman, D. G.; Wulfeck-Kleier, K. A.; Pressman, J. G.
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Authors: Berney, M.; Hammes, F.; Bosshard, F.; Weilenmann, H. U.; Egli, T.
Journal: Appl Environ Microbiol (2007): 3283-90
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