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MycoLight™ Bacterial Viability Assay Kit
AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell. The kit utilizes the mixture of our green fluorescent nucleic acid stain MycoLight™ Green and the red-fluorescent nucleic acid stain propidium iodide. When used alone, the MycoLight™ Green stain generally labels all bacteria (live and dead) in a population. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a reduction in the MycoLight™ Green stain fluorescence when both dyes are present. Thus, with an appropriate mixture of the MycoLight™ Green and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead or dying bacteria with damaged membranes gives red fluorescence. The Mycolight ™ Bacterial Viability Assay Kit is a robust tool for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell. Stained cells can be monitored fluorimetrically at 510-530 nm (FITC filter) and 600-660 nm (Texas red filter) with excitation at 488 nm, the most common excitation light source.
<p>Fluorescence images of <em>E. coli</em> HST08 stained with Cell Meter&trade; Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead <em>E.coli</em> bacterial cells were also visualized in a mixed population (Middle). </p>
<p>Fluorescence images of <em>E. coli</em> HST08 stained with Cell Meter&trade; Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead <em>E.coli</em> bacterial cells were also visualized in a mixed population (Middle). </p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22400200 Tests
Price
 
Spectral properties
Excitation (nm)482
Emission (nm)512
Storage, safety and handling
H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm, 610/20 nm filter
Instrument specification(s)FITC, PE-Texas Red channel
Fluorescence microscope
Excitation510/600 nm
Emission530/660 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC/Texas Red filter sets
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Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Page updated on September 30, 2025