MycoLight™ Bacterial Viability Assay Kit
Overview | ![]() ![]() |
Excitation (nm) 482 | Emission (nm) 512 |
AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell. The kit utilizes the mixture of our green fluorescent nucleic acid stain MycoLight™ Green and the red-fluorescent nucleic acid stain propidium iodide. When used alone, the MycoLight™ Green stain generally labels all bacteria (live and dead) in a population. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a reduction in the MycoLight™ Green stain fluorescence when both dyes are present. Thus, with an appropriate mixture of the MycoLight™ Green and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead or dying bacteria with damaged membranes gives red fluorescence. The Mycolight ™ Bacterial Viability Assay Kit is a robust tool for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell. Stained cells can be monitored fluorimetrically at 510-530 nm (FITC filter) and 600-660 nm (Texas red filter) with excitation at 488 nm, the most common excitation light source.
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm, 610/20 nm filter |
Instrument specification(s) | FITC, PE-Texas Red channel |
Fluorescence microscope
Excitation | 510/600 nm |
Emission | 530/660 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | FITC/Texas Red filter sets |
Components
Component A: MycoLight™ Green | 1 vial (200 μL) |
Component B: Propidium iodide | 1 vial (200 μL) |
Product family
Images
Figure 1. Fluorescence images of E. coli HST08 stained with Cell Meter™ Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead E.coli bacterial cells were also visualized in a mixed population (Middle).
References
View all 7 references: Citation Explorer
Applicability of LIVE/DEAD BacLight stain with glutaraldehyde fixation for the measurement of bacterial abundance and viability in rainwater
Authors: Hu, W.; Murata, K.; Zhang, D.
Journal: J Environ Sci (China) (2017): 202-213
Authors: Hu, W.; Murata, K.; Zhang, D.
Journal: J Environ Sci (China) (2017): 202-213
the BacLight Live/Dead viability assay and other vital dyes
Authors: Karkashan, A.; Khallaf, B.; Morris, J.; Thurbon, N.; Rouch, D.; Smith, S. R.; Deighton, M., Comparison of methodologies for enumerating and detecting the viability of Ascaris eggs in sewage sludge by st, undefined and ard incubation-microscopy, undefined
Journal: Water Res (2015): 533-44
Authors: Karkashan, A.; Khallaf, B.; Morris, J.; Thurbon, N.; Rouch, D.; Smith, S. R.; Deighton, M., Comparison of methodologies for enumerating and detecting the viability of Ascaris eggs in sewage sludge by st, undefined and ard incubation-microscopy, undefined
Journal: Water Res (2015): 533-44
Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods
Authors: Wahman, D. G.; Schrantz, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2010): 8277-80
Authors: Wahman, D. G.; Schrantz, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2010): 8277-80
Monochloramine disinfection kinetics of Nitrosomonas europaea by propidium monoazide quantitative PCR and Live/dead BacLight methods
Authors: Wahman, D. G.; Wulfeck-Kleier, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2009): 5555-62
Authors: Wahman, D. G.; Wulfeck-Kleier, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2009): 5555-62
Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry
Authors: Berney, M.; Hammes, F.; Bosshard, F.; Weilenmann, H. U.; Egli, T.
Journal: Appl Environ Microbiol (2007): 3283-90
Authors: Berney, M.; Hammes, F.; Bosshard, F.; Weilenmann, H. U.; Egli, T.
Journal: Appl Environ Microbiol (2007): 3283-90
Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples
Authors: Leuko, S.; Legat, A.; Fendrihan, S.; Stan-Lotter, H.
Journal: Appl Environ Microbiol (2004): 6884-6
Authors: Leuko, S.; Legat, A.; Fendrihan, S.; Stan-Lotter, H.
Journal: Appl Environ Microbiol (2004): 6884-6
LIVE/DEAD BacLight : application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water
Authors: Boulos, L.; Prevost, M.; Barbeau, B.; Coallier, J.; Desjardins, R.
Journal: J Microbiol Methods (1999): 77-86
Authors: Boulos, L.; Prevost, M.; Barbeau, B.; Coallier, J.; Desjardins, R.
Journal: J Microbiol Methods (1999): 77-86