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AAT Bioquest

MycoLight™ Bacterial Viability Assay Kit

AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell. The kit utilizes the mixture of our green fluorescent nucleic acid stain MycoLight™ Green and the red-fluorescent nucleic acid stain propidium iodide. When used alone, the MycoLight™ Green stain generally labels all bacteria (live and dead) in a population. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a reduction in the MycoLight™ Green stain fluorescence when both dyes are present. Thus, with an appropriate mixture of the MycoLight™ Green and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead or dying bacteria with damaged membranes gives red fluorescence. The Mycolight ™ Bacterial Viability Assay Kit is a robust tool for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell. Stained cells can be monitored fluorimetrically at 510-530 nm (FITC filter) and 600-660 nm (Texas red filter) with excitation at 488 nm, the most common excitation light source.

Spectrum

Citations

View all 4 citations: Citation Explorer
An epigallocatechin gallate--amorphous calcium phosphate nanocomposite for caries prevention and demineralized enamel restoration
Authors: Dai, Danni and Wang, Jianrong and Xie, Hanshu and Zhang, Chao
Journal: Materials Today Bio (2023): 100715
Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures
Authors: Biagini, Francesco and Calvigioni, Marco and De Maria, Carmelo and Magliaro, Chiara and Montemurro, Francesca and Mazzantini, Diletta and Celandroni, Francesco and Mattioli-Belmonte, Monica and Ghelardi, Emilia and Vozzi, Giovanni
Journal: Bioengineering (2022): 96
Construction of Iron-Scavenging Hydrogel via Thiol--Ene Click Chemistry for Antibiotic-Free Treatment of Bacterial Wound Infection
Authors: Xie, Tian-Qiu and Yan, Xiao and Yan, Jian-Hua and Yu, Yun-Jian and Liu, Xin-Hua and Feng, Jun and Liu, Chuan-Jun and Zhang, Xian-Zheng
Journal: Advanced Healthcare Materials : 2401118
Low-friction soft robots for targeted bacterial infection treatment in gastrointestinal tract
Authors: Wang, Ben and Chen, Yunrui and Ye, Zhicheng and Yu, Haidong and Chan, Kai Fung and Xu, Tiantian and Guo, Zhiguang and Liu, Weimin and Zhang, Li
Journal: Cyborg and Bionic Systems

References

View all 7 references: Citation Explorer
Applicability of LIVE/DEAD BacLight stain with glutaraldehyde fixation for the measurement of bacterial abundance and viability in rainwater
Authors: Hu, W.; Murata, K.; Zhang, D.
Journal: J Environ Sci (China) (2017): 202-213
the BacLight Live/Dead viability assay and other vital dyes
Authors: Karkashan, A.; Khallaf, B.; Morris, J.; Thurbon, N.; Rouch, D.; Smith, S. R.; Deighton, M., Comparison of methodologies for enumerating and detecting the viability of Ascaris eggs in sewage sludge by st, undefined and ard incubation-microscopy, undefined
Journal: Water Res (2015): 533-44
Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods
Authors: Wahman, D. G.; Schrantz, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2010): 8277-80
Monochloramine disinfection kinetics of Nitrosomonas europaea by propidium monoazide quantitative PCR and Live/dead BacLight methods
Authors: Wahman, D. G.; Wulfeck-Kleier, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2009): 5555-62
Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry
Authors: Berney, M.; Hammes, F.; Bosshard, F.; Weilenmann, H. U.; Egli, T.
Journal: Appl Environ Microbiol (2007): 3283-90
Page updated on November 3, 2024

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Catalog Number22400
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Spectral properties

Excitation (nm)

482

Emission (nm)

512

Storage, safety and handling

H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25
UNSPSC12352200

Platform

Flow cytometer

Excitation488 nm laser
Emission530, 30 nm, 610, 20 nm filter
Instrument specification(s)FITC, PE-Texas Red channel

Fluorescence microscope

Excitation510, 600 nm
Emission530, 660 nm
Recommended plateBlack wall, clear bottom
Instrument specification(s)FITC, Texas Red filter sets

Components

Fluorescence images of <em>E. coli</em> HST08 stained with Cell Meter&trade; Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead <em>E.coli</em> bacterial cells were also visualized in a mixed population (Middle).
Fluorescence images of <em>E. coli</em> HST08 stained with Cell Meter&trade; Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead <em>E.coli</em> bacterial cells were also visualized in a mixed population (Middle).
Fluorescence images of <em>E. coli</em> HST08 stained with Cell Meter&trade; Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead <em>E.coli</em> bacterial cells were also visualized in a mixed population (Middle).