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MycoLight™ Bacterial Viability Assay Kit

Fluorescence images of <em>E. coli</em> HST08 stained with Cell Meter™ Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead <em>E.coli</em> bacterial cells were also visualized in a mixed population (Middle).
Fluorescence images of <em>E. coli</em> HST08 stained with Cell Meter™ Bacterial Viability Assay Kit (Cat#22400). Live bacteria with intact cell membranes showed green fluorescence (Left), while 70% alcohol-killed dead bacteria (Right) with compromised membranes showed red fluorescence. Live and dead <em>E.coli</em> bacterial cells were also visualized in a mixed population (Middle).
Ordering information
Price ()
Catalog Number22400
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Spectral properties
Excitation (nm)482
Emission (nm)512
Storage, safety and handling
H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Excitation (nm)
482
Emission (nm)
512
AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell. The kit utilizes the mixture of our green fluorescent nucleic acid stain MycoLight™ Green and the red-fluorescent nucleic acid stain propidium iodide. When used alone, the MycoLight™ Green stain generally labels all bacteria (live and dead) in a population. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a reduction in the MycoLight™ Green stain fluorescence when both dyes are present. Thus, with an appropriate mixture of the MycoLight™ Green and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead or dying bacteria with damaged membranes gives red fluorescence. The Mycolight ™ Bacterial Viability Assay Kit is a robust tool for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell. Stained cells can be monitored fluorimetrically at 510-530 nm (FITC filter) and 600-660 nm (Texas red filter) with excitation at 488 nm, the most common excitation light source.

Platform


Flow cytometer

Excitation488 nm laser
Emission530/30 nm, 610/20 nm filter
Instrument specification(s)FITC, PE-Texas Red channel

Fluorescence microscope

Excitation510/600 nm
Emission530/660 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC/Texas Red filter sets

Components


Component A: MycoLight™ Green1 vial (200 μL)
Component B: Propidium iodide1 vial (200 μL)

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Excitation (nm)482
Emission (nm)512

References


View all 7 references: Citation Explorer
Applicability of LIVE/DEAD BacLight stain with glutaraldehyde fixation for the measurement of bacterial abundance and viability in rainwater
Authors: Hu, W.; Murata, K.; Zhang, D.
Journal: J Environ Sci (China) (2017): 202-213
the BacLight Live/Dead viability assay and other vital dyes
Authors: Karkashan, A.; Khallaf, B.; Morris, J.; Thurbon, N.; Rouch, D.; Smith, S. R.; Deighton, M., Comparison of methodologies for enumerating and detecting the viability of Ascaris eggs in sewage sludge by st, undefined and ard incubation-microscopy, undefined
Journal: Water Res (2015): 533-44
Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods
Authors: Wahman, D. G.; Schrantz, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2010): 8277-80
Monochloramine disinfection kinetics of Nitrosomonas europaea by propidium monoazide quantitative PCR and Live/dead BacLight methods
Authors: Wahman, D. G.; Wulfeck-Kleier, K. A.; Pressman, J. G.
Journal: Appl Environ Microbiol (2009): 5555-62
Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry
Authors: Berney, M.; Hammes, F.; Bosshard, F.; Weilenmann, H. U.; Egli, T.
Journal: Appl Environ Microbiol (2007): 3283-90
Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples
Authors: Leuko, S.; Legat, A.; Fendrihan, S.; Stan-Lotter, H.
Journal: Appl Environ Microbiol (2004): 6884-6
LIVE/DEAD BacLight : application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water
Authors: Boulos, L.; Prevost, M.; Barbeau, B.; Coallier, J.; Desjardins, R.
Journal: J Microbiol Methods (1999): 77-86