PE-Cy7 Tandem
PE-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 780 nm.AAT Bioquest also offers a unique preactivated PE-Cy7 to facilitate the PE-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
![Jurkat cells were incubated with biotinylated-Anti-CD45 followed by Streptavidin-PE/Cy7 stain.<br>Blue: Control<br>Red: Streptavidin-PE/Cy7 prepared with PE/Cy7 Tandem<br>Green: Streptavidin-PE/Cy7 (products of Company B)](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-cy7-tandem%2Ffigure-for-pe-cy7-tandem_ttAYD.jpg&w=640&q=75)
![Jurkat cells were incubated with biotinylated-Anti-CD45 followed by Streptavidin-PE/Cy7 stain.<br>Blue: Control<br>Red: Streptavidin-PE/Cy7 prepared with PE/Cy7 Tandem<br>Green: Streptavidin-PE/Cy7 (products of Company B)](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-cy7-tandem%2Ffigure-for-pe-cy7-tandem_ttAYD.jpg&w=640&q=75)
![Jurkat cells were incubated with biotinylated-Anti-CD45 followed by Streptavidin-PE/Cy7 stain.<br>Blue: Control<br>Red: Streptavidin-PE/Cy7 prepared with PE/Cy7 Tandem<br>Green: Streptavidin-PE/Cy7 (products of Company B)](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-cy7-tandem%2Ffigure-for-pe-cy7-tandem_ttAYD.jpg&w=128&q=25)
![Flow cytometry analysis of whole blood stained with PE-Cy7 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-Cy7 specific B14-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fpe-cy7-tandem%2Ffigure-for-pe-cy7-tandem_7mk7s.png&w=128&q=25)
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
PE-Cy5 Tandem | 565 | 666 | 1960000 |
APC-Cy7 Tandem | 651 | 779 | 700000 |
References
View all 46 references: Citation Explorer
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59