Pluronic® F127 is a detergent that can improve the water solubility of hydrophobic molecules, such as AM esters and lipophilic tracers.

 

Pluronic® F-127, also known as poloxamer 407, is a surfactant polyol (polyoxypropylene-polyoxyethylene block copolymer). It has a molecular weight of approximately 13 kDa and is provided as a 10% solution in water.

 

Given its amphiphilic nature, Pluronic® F127 has been found to help solubilize and disperse dye molecules in physiological media, such as Hanks' Balanced Salt Solution (HBSS). Data shows that it is relatively non-toxic to cells. ^{[1, 2]}

 

While concentration is often experimentally dependent, this table provides a guideline for some common Pluronic® F-127 uses:

Compound	Pluronic® F127 Concentration
Calcein AM	0.02%
CFDA AM	0.05%
Fluo-3 AM	0.04%
Fluo-4 AM	0.04%
Fura-2 AM	0.04%
Rhod-2 AM	0.04%