Pluronic® F-127

10% solution in water

Pluronic® F-127 (10% Solution) is an amphiphilic surfactant that enhances the solubility and dispersion of hydrophobic molecules like AM esters and lipophilic dyes in physiological media.

Dispersal Agent: PEO-PPO-PEO triblock copolymer solubilizes hydrophobic dyes
Low CMC Design: Forms micelles at 0.2–1% to prevent dye aggregation
Cell Loading Enhancer: Facilitates AM ester delivery across plasma membranes
Ready-to-Use Format: Pre-dissolved 10% solution eliminates preparation variability

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20053	10 mL	Price

Overview

Pluronic® F127 is a detergent that can improve the water solubility of hydrophobic molecules, such as AM esters and lipophilic tracers.

Pluronic® F-127, also known as poloxamer 407, is a surfactant polyol (polyoxypropylene-polyoxyethylene block copolymer). It has a molecular weight of approximately 13 kDa and is provided as a 10% solution in water.

Given its amphiphilic nature, Pluronic® F127 has been found to help solubilize and disperse dye molecules in physiological media, such as Hanks' Balanced Salt Solution (HBSS). Data shows that it is relatively non-toxic to cells. ^{[1, 2]}

While concentration is often experimentally dependent, this table provides a guideline for some common Pluronic® F-127 uses:

Compound	Pluronic® F127 Concentration
Calcein AM	0.02%
CFDA AM	0.05%
Fluo-3 AM	0.04%
Fluo-4 AM	0.04%
Fura-2 AM	0.04%
Rhod-2 AM	0.04%

Hamad, M. I., Krause, M., & Wahle, P. (2015). Improving AM ester calcium dye loading efficiency. Journal of neuroscience methods, 240, 48-60.
Poenie, M., Alderton, J., Steinhardt, R., & Tsien, R. (1986). Calcium rises abruptly and briefly throughout the cell at the onset of anaphase. Science, 233(4766), 886-889.

Citations

View all 25 citations: Citation Explorer

Mitochondrial-dependent oxidative phosphorylation is key for postnatal metabolic adaptation of alveolar macrophages in the lung

Authors:

Zhang, Jun and Peng, Yu and Song, Haosen and Liu, Siqi and Li, Chuanwei and Zhang, Yi and Shi, Xiaowei and Guo, Huifang and Xu, Yingping

Journal:

International Immunopharmacology (2024): 112012

Agonist-Induced Ca2+ Signaling in HEK-293-Derived Cells Expressing a Single IP3 Receptor Isoform

Authors:

Kochkina, Ekaterina N and Kopylova, Elizaveta Е and Rogachevskaja, Olga A and Kovalenko, Nina P and Kabanova, Natalia V and Kotova, Polina D and Bystrova, Marina F and Kolesnikov, Stanislav S

Journal:

Cells (2024): 562

FTO-mediated m6A demethylation regulates GnRH expression in the hypothalamus via the PLC$\beta$3/Ca2+/CAMK signalling pathway

Authors:

Zang, Shaolian and Yin, Xiaoqin and Li, Pin

Journal:

Communications Biology (2023): 1297

CVD-associated SNPs with regulatory potential reveal novel non-coding disease genes

Authors:

Zhu, Chaonan and Baumgarten, Nina and Wu, Meiqian and Wang, Yue and Das, Arka Provo and Kaur, Jaskiran and Ardakani, Fatemeh Behjati and Duong, Thanh Thuy and Pham, Minh Duc and Duda, Maria and others,

Journal:

Human Genomics (2023): 1--17

Analysis of Fatty Acid Metabolism in Fetal and Failing Hearts by Single-Cell RNA Sequencing Revealed SLC27A6 as a Critical Gene in Heart Maturation

Authors:

Zhu, Wenjia and Zheng, Yufan and Liu, Jiaying and Zhao, Chao and Sun, Ning and Qu, Xiuxia and Yang, Hui

Journal:

Acta Cardiologica Sinica (2023): 580

Physical properties

Molecular weight	~13000
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Room temperature (10-25 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 18, 2024