Pluronic® F-127

10% solution in water

Pluronic® F-127 (10% Solution) is an amphiphilic surfactant that enhances the solubility and dispersion of hydrophobic molecules like AM esters and lipophilic dyes in physiological media.

Dispersal Agent: PEO-PPO-PEO triblock copolymer solubilizes hydrophobic dyes
Low CMC Design: Forms micelles at 0.2–1% to prevent dye aggregation
Cell Loading Enhancer: Facilitates AM ester delivery across plasma membranes
Ready-to-Use Format: Pre-dissolved 10% solution eliminates preparation variability

Datasheet

SDS

Catalog	Size	Price	Quantity
20053	10 mL	Price

Overview

Pluronic® F127 is a detergent that can improve the water solubility of hydrophobic molecules, such as AM esters and lipophilic tracers.

Pluronic® F-127, also known as poloxamer 407, is a surfactant polyol (polyoxypropylene-polyoxyethylene block copolymer). It has a molecular weight of approximately 13 kDa and is provided as a 10% solution in water.

Given its amphiphilic nature, Pluronic® F127 has been found to help solubilize and disperse dye molecules in physiological media, such as Hanks' Balanced Salt Solution (HBSS). Data shows that it is relatively non-toxic to cells. ^{[1, 2]}

While concentration is often experimentally dependent, this table provides a guideline for some common Pluronic® F-127 uses:

Compound	Pluronic® F127 Concentration
Calcein AM	0.02%
CFDA AM	0.05%
Fluo-3 AM	0.04%
Fluo-4 AM	0.04%
Fura-2 AM	0.04%
Rhod-2 AM	0.04%

Hamad, M. I., Krause, M., & Wahle, P. (2015). Improving AM ester calcium dye loading efficiency. Journal of neuroscience methods, 240, 48-60.
Poenie, M., Alderton, J., Steinhardt, R., & Tsien, R. (1986). Calcium rises abruptly and briefly throughout the cell at the onset of anaphase. Science, 233(4766), 886-889.

Citations

View all 28 citations: Citation Explorer

Cardiac Organoids Uncover an Association of CHD7 Deficiency with Congenital Heart Disease

Authors:

Sun, Siyu and Feng, Zhiyu and Na, Weilan and Gao, Yuan and Min, Shaojie and Zhuang, Quannan and Lin, Siyi and Zhao, Quming and Huang, Xianghui and Song, Hongmei and others,

Journal:

Phenomics (2026): 1--16

A high-throughput assay for the measurement of Ca2+-oscillations and insulin release from uniformly sized $\beta$-cell spheroids

Authors:

Robben, Stijn and Davidson, Patricia and Ribeiro, Rita S Rodrigues and Voets, Thomas

Journal:

eLife (2026)

Western Diet-Induced Impairment of Left Atrium Cardiomyocyte Contractility in Female Wistar Rats Is Associated with Slowdown in the Cross-Bridge Cycle and Dephosphorylation of cMyBP-C

Authors:

Mukhlynina, Elena and Butova, Xenia and Myachina, Tatiana and Simonova, Raisa and Antonets, Yulia and Leiberova, Anna and Kochurova, Anastasia and Gusarova, Evgeniya and Chumarnaya, Tatiana and Kopylova, Galina and others,

Journal:

International Journal of Molecular Sciences (2026): 1508

Mitochondrial-dependent oxidative phosphorylation is key for postnatal metabolic adaptation of alveolar macrophages in the lung

Authors:

Zhang, Jun and Peng, Yu and Song, Haosen and Liu, Siqi and Li, Chuanwei and Zhang, Yi and Shi, Xiaowei and Guo, Huifang and Xu, Yingping

Journal:

International Immunopharmacology (2024): 112012

Agonist-Induced Ca2+ Signaling in HEK-293-Derived Cells Expressing a Single IP3 Receptor Isoform

Authors:

Kochkina, Ekaterina N and Kopylova, Elizaveta Е and Rogachevskaja, Olga A and Kovalenko, Nina P and Kabanova, Natalia V and Kotova, Polina D and Bystrova, Marina F and Kolesnikov, Stanislav S

Journal:

Cells (2024): 562

Physical properties

Molecular weight	~13000
Solvent	Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Room temperature (10-25 °C); Minimize light exposure

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Page updated on September 18, 2024