Name: Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit *Optimized for CytoCite™ and Qubit™ Fluorometers*
Brand: AAT Bioquest
SKU: 17660
Availability: InStock

Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit

Optimized for CytoCite™ and Qubit™ Fluorometers

Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit is a fluorimetric assay kit for quantifying double-stranded DNA (dsDNA) using Helixyte™ Green with CytoCite™ or Qubit™ fluorometers.

Detection Principle: Helixyte™ Green increases fluorescence upon binding dsDNA.
Sensitivity: Demonstrated detection limit of 10 pg/µL dsDNA.
Selectivity: Highly selective for dsDNA over RNA.
Dynamic Range: Provides linear response over five orders of magnitude.
Workflow: Requires a 2-minute room-temperature incubation before reading at Ex/Em 480/520 nm.

DNA standard curve generated using Portelite™ Fluorimetric DNA High Sensitivity Quantitation Kit. Fluorescence intensity was quantified using FITC channel, regression model was calculated using log-log best-fit. Detection limit was 10 pg/µL.

Protocol

COA

Catalog	Size	Price	Quantity
17660	100 Tests	Price
17661	500 Tests	Price

Overview

DNA Quantitation is a very important task in DNA sample preparations for various genomic analyses. This Portelite™ dsDNA Quantitation Kit provides a rapid method to quantify dsDNA with Helixyte™ Green probe using a hand-held fluorometer. It is optimized for Cytocite™ and Qubit™ fluorometers. Portelite™ dsDNA Quantitation assay is linear over five orders of magnitude. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 1.25 pg/uL to 10 ng/uL. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA, and it is a few magnitudes more sensitive than UV absorbance readings.

Components

Catalog 17660 (100 Tests)

Component A: Helixyte™ Green (200X)	1 vial (0.25 mL-200X in DMSO)
Component B: DNA Assay Buffer	1 bottle (50 mL)
Component C: DNA Standard #1	1 vial (1 mL, Calf thymus DNA: 0 ng/µL)
Component D: DNA Standard #2	1 vial (1 mL, Calf thymus DNA: 10 ng/µL)

Catalog 17661 (500 Tests)

Component A: Helixyte™ Green	1 vial (1.25 mL-200X in DMSO)
Component B: DNA Assay Buffer	250 mL (3 bottles - 85 mL each)
Component C: DNA Standard #1	1 bottle (5 mL), Calf thymus DNA: 0 ng/µL)
Component D: DNA Standard #2	1 bottle (5 mL), Calf thymus DNA: 10 ng/µL)

Citations

View all 3 citations: Citation Explorer

Local antibody feedback enforces a checkpoint on affinity maturation in the germinal center and promotes epitope spreading

Authors:

Yan, Yu and Wang, Xuesong and Xie, Zhenfei and Bader, Daniel LV and Lim, Ryan H and Ma, Krystal M and Cottrell, Christopher A and Steichen, Jon M and Xu, Liling and Villavicencio, Paula M and others,

Journal:

Immunity (2026)

Abelmoschi Corolla polysaccharides and related metabolite ameliorates colitis via modulating gut microbiota and regulating the FXR/STAT3 signaling pathway

Authors:

He, Linhai and Chen, Kaidi and Chen, Zepeng and Chen, Chen and Zhou, Jin and Shao, Yifan and Ma, Jiaze and Qiu, Zhengxi and Chen, Yugen and Zhang, Wei

Journal:

International Journal of Biological Macromolecules (2024): 134370

Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1/nuclear factor-erythroid-2-related factor 2/heme oxygenase-1 signaling pathway

Authors:

Chen, Kai-Di and Wang, Kui-Ling and Chen, Chen and Zhu, Yi-Jia and Tang, Wen-Wen and Wang, Yu-Ji and Chen, Ze-Peng and He, Lin-Hai and Chen, Yu-Gen and Zhang, Wei

Journal:

World Journal of Gastroenterology (2024): 2709--2725

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Qubit Fluorometer
Excitation	480 nm
Emission	520 nm
Instrument specification(s)	0.2 mL, thin-wall PCR tube

CytoCite Fluorometer
Excitation	480 nm
Emission	520 nm
Instrument specification(s)	0.2 mL, thin-wall PCR tube

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026