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Protonex™ Green 500WS
Protonex™ Green dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Green dye. The fluorescence of Protonex™ Green dye significantly increases as pH decreases from neutral to the acidic. Protonex™ Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes, lysosomes and other biological targets. Protonex™ Green 500 WS is not permeable to live cells, enabling the specific detection of extracellular acidic biological targets. Protonex™ Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of Protonex™ Green.
Emission Spectra of Protonex™ Green 500 at different pH.
Emission Spectra of Protonex™ Green 500 at different pH.
CatalogSize
Price
Quantity
212181 mg
Price
 
Physical properties

Molecular weight775.63
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
4000
Excitation (nm)445
Emission (nm)503
Storage, safety and handling

Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on October 13, 2025