Protonex™ Green 500WS

Protonex™ Green dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Green dye. The fluorescence of Protonex™ Green dye significantly increases as pH decreases from neutral to the acidic. Protonex™ Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes, lysosomes and other biological targets. Protonex™ Green 500 WS is not permeable to live cells, enabling the specific detection of extracellular acidic biological targets. Protonex™ Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of Protonex™ Green.

Emission Spectra of Protonex™ Green 500 at different pH.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21218	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Green 500WS to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	128.927 µL	644.637 µL	1.289 mL	6.446 mL	12.893 mL
5 mM	25.785 µL	128.927 µL	257.855 µL	1.289 mL	2.579 mL
10 mM	12.893 µL	64.464 µL	128.927 µL	644.637 µL	1.289 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	775.63 g/mol	=	mL	x	mM	=	mmol

Citations

View all 20 citations: Citation Explorer

Starch-regulated copper-terephthalic acid as a pH/hydrogen peroxide simultaneous-responsive fluorescent probe for lysosome imaging

Authors:

Chen, J., Si, Y., Liu, Y., Wang, S., Wang, S., Zhang, Y., Yang, B., Zhang, Z., Zhang, S.

Journal:

Dalton Trans (2019): 13017-13025

A structure optimized fluorescent probe for highly sensitive monitoring drug induced lysosomal pH value changes

Authors:

Gong, Y. J., Kong, Z. Z., Zhang, M. L., Lv, M. K., Zhang, G.

Journal:

Talanta (2019): 1-8

An effective FRET-based two-photon ratiometric fluorescent probe with double well-resolved emission bands for lysosomal pH changes in living cells and zebrafish

Authors:

Yuan, G., Ding, H., Zhou, L.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2019): 117397

A pH probe inhibits senescence in mesenchymal stem cells

Authors:

Wang, L., Han, X., Qu, G., Su, L., Zhao, B., Miao, J.

Journal:

Stem Cell Res Ther (2018): 343

Dual site-controlled two-photon fluorescent probe for the imaging of lysosomal pH in living cells

Authors:

Wang, C., Dong, B., Kong, X., Zhang, N., Song, W., Lin, W.

Journal:

Luminescence (2018): 1275-1280

Physical properties

Molecular weight	775.63
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	4000
Excitation (nm)	445
Emission (nm)	503

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 30, 2025