Protonex™ Green 500WS

Emission Spectra of Protonex™ Green 500 at different pH.

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	775.63
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	4000
Excitation (nm)	445
Emission (nm)	503

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

775.63

Extinction coefficient (cm ^-1 M ^-1)

4000

Excitation (nm)

445

Emission (nm)

503

Protonex™ Green dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Green dye. The fluorescence of Protonex™ Green dye significantly increases as pH decreases from neutral to the acidic. Protonex™ Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes, lysosomes and other biological targets. Protonex™ Green 500 WS is not permeable to live cells, enabling the specific detection of extracellular acidic biological targets. Protonex™ Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of Protonex™ Green.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Green 500WS to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	128.927 µL	644.637 µL	1.289 mL	6.446 mL	12.893 mL
5 mM	25.785 µL	128.927 µL	257.855 µL	1.289 mL	2.579 mL
10 mM	12.893 µL	64.464 µL	128.927 µL	644.637 µL	1.289 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	4000
Excitation (nm)	445
Emission (nm)	503

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
Protonex™ Green 500	445	503	4000

Images

Figure 1. Emission Spectra of Protonex™ Green 500 at different pH.

Citations

View all 20 citations: Citation Explorer

Starch-regulated copper-terephthalic acid as a pH/hydrogen peroxide simultaneous-responsive fluorescent probe for lysosome imaging
Authors: Chen, J., Si, Y., Liu, Y., Wang, S., Wang, S., Zhang, Y., Yang, B., Zhang, Z., Zhang, S.
Journal: Dalton Trans (2019): 13017-13025

A structure optimized fluorescent probe for highly sensitive monitoring drug induced lysosomal pH value changes
Authors: Gong, Y. J., Kong, Z. Z., Zhang, M. L., Lv, M. K., Zhang, G.
Journal: Talanta (2019): 1-8

An effective FRET-based two-photon ratiometric fluorescent probe with double well-resolved emission bands for lysosomal pH changes in living cells and zebrafish
Authors: Yuan, G., Ding, H., Zhou, L.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 117397

A pH probe inhibits senescence in mesenchymal stem cells
Authors: Wang, L., Han, X., Qu, G., Su, L., Zhao, B., Miao, J.
Journal: Stem Cell Res Ther (2018): 343

Dual site-controlled two-photon fluorescent probe for the imaging of lysosomal pH in living cells
Authors: Wang, C., Dong, B., Kong, X., Zhang, N., Song, W., Lin, W.
Journal: Luminescence (2018): 1275-1280

A lysosome-locating and acidic pH-activatable fluorescent probe for visualizing endogenous H2O2 in lysosomes
Authors: Liu, J., Zhou, S., Ren, J., Wu, C., Zhao, Y.
Journal: Analyst (2017): 4522-4528

Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic pH-activatable fluorescent probe
Authors: Xie, X., Tang, F., Shangguan, X., Che, S., Niu, J., Xiao, Y., Wang, X., Tang, B.
Journal: Chem Commun (Camb) (2017): 6520-6523

A fluorescent pH probe for acidic organelles in living cells
Authors: Chen, J. W., Chen, C. M., Chang, C. C.
Journal: Org Biomol Chem (2017): 7936-7943

Near-Infrared Probe Based on Rhodamine Derivative for Highly Sensitive and Selective Lysosomal pH Tracking
Authors: Niu, G., Zhang, P., Liu, W., Wang, M., Zhang, H., Wu, J., Zhang, L., Wang, P.
Journal: Anal Chem (2017): 1922-1929

Selective imaging of cancer cells with a pH-activatable lysosome-targeting fluorescent probe
Authors: Shi, R., Huang, L., Duan, X., Sun, G., Yin, G., Wang, R., Zhu, J. J.
Journal: Anal Chim Acta (2017): 66-73