logo
AAT Bioquest

Protonex™ Green 500WS

Emission Spectra of Protonex™ Green 500 at different pH.
Emission Spectra of Protonex™ Green 500 at different pH.
Emission Spectra of Protonex™ Green 500 at different pH.
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight775.63
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)4000
Excitation (nm)445
Emission (nm)503
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
775.63
Extinction coefficient (cm -1 M -1)
4000
Excitation (nm)
445
Emission (nm)
503
Protonex™ Green dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Green dye. The fluorescence of Protonex™ Green dye significantly increases as pH decreases from neutral to the acidic. Protonex™ Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes, lysosomes and other biological targets. Protonex™ Green 500 WS is not permeable to live cells, enabling the specific detection of extracellular acidic biological targets. Protonex™ Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of Protonex™ Green.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Green 500WS to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM128.927 µL644.637 µL1.289 mL6.446 mL12.893 mL
5 mM25.785 µL128.927 µL257.855 µL1.289 mL2.579 mL
10 mM12.893 µL64.464 µL128.927 µL644.637 µL1.289 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)4000
Excitation (nm)445
Emission (nm)503

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Protonex™ Green 5004455034000

Images


Citations


View all 20 citations: Citation Explorer
Starch-regulated copper-terephthalic acid as a pH/hydrogen peroxide simultaneous-responsive fluorescent probe for lysosome imaging
Authors: Chen, J., Si, Y., Liu, Y., Wang, S., Wang, S., Zhang, Y., Yang, B., Zhang, Z., Zhang, S.
Journal: Dalton Trans (2019): 13017-13025
A structure optimized fluorescent probe for highly sensitive monitoring drug induced lysosomal pH value changes
Authors: Gong, Y. J., Kong, Z. Z., Zhang, M. L., Lv, M. K., Zhang, G.
Journal: Talanta (2019): 1-8
An effective FRET-based two-photon ratiometric fluorescent probe with double well-resolved emission bands for lysosomal pH changes in living cells and zebrafish
Authors: Yuan, G., Ding, H., Zhou, L.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 117397
A pH probe inhibits senescence in mesenchymal stem cells
Authors: Wang, L., Han, X., Qu, G., Su, L., Zhao, B., Miao, J.
Journal: Stem Cell Res Ther (2018): 343
Dual site-controlled two-photon fluorescent probe for the imaging of lysosomal pH in living cells
Authors: Wang, C., Dong, B., Kong, X., Zhang, N., Song, W., Lin, W.
Journal: Luminescence (2018): 1275-1280
A lysosome-locating and acidic pH-activatable fluorescent probe for visualizing endogenous H2O2 in lysosomes
Authors: Liu, J., Zhou, S., Ren, J., Wu, C., Zhao, Y.
Journal: Analyst (2017): 4522-4528
Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic pH-activatable fluorescent probe
Authors: Xie, X., Tang, F., Shangguan, X., Che, S., Niu, J., Xiao, Y., Wang, X., Tang, B.
Journal: Chem Commun (Camb) (2017): 6520-6523
A fluorescent pH probe for acidic organelles in living cells
Authors: Chen, J. W., Chen, C. M., Chang, C. C.
Journal: Org Biomol Chem (2017): 7936-7943
Near-Infrared Probe Based on Rhodamine Derivative for Highly Sensitive and Selective Lysosomal pH Tracking
Authors: Niu, G., Zhang, P., Liu, W., Wang, M., Zhang, H., Wu, J., Zhang, L., Wang, P.
Journal: Anal Chem (2017): 1922-1929
Selective imaging of cancer cells with a pH-activatable lysosome-targeting fluorescent probe
Authors: Shi, R., Huang, L., Duan, X., Sun, G., Yin, G., Wang, R., Zhu, J. J.
Journal: Anal Chim Acta (2017): 66-73