Protonex™ Red 670, NHS ester

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	827.02
Solvent	DMSO

Spectral properties

Excitation (nm)	643
Emission (nm)	660

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

See also: Intracellular pH

Molecular weight

827.02

Excitation (nm)

643

Emission (nm)

660

Protonex™ Red 670 NHS ester is an excellent building block for developing a target-specific conjugate that can be used for monitoring a pH-sensitive biological event. Protonex™ Red 670 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 670 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 670 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 670 to become highly fluorescent and emit red light when excited by a red laser such as a 632 nm He-Ne or 647 nm red laser. By measuring the fluorescence intensity of Protonex™ Red 670, one can monitor the activation and trafficking of receptors or antibodies in live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 670, NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	120.916 µL	604.58 µL	1.209 mL	6.046 mL	12.092 mL
5 mM	24.183 µL	120.916 µL	241.832 µL	1.209 mL	2.418 mL
10 mM	12.092 µL	60.458 µL	120.916 µL	604.58 µL	1.209 mL