Name: Protonex™ Red 780, NHS ester
Brand: AAT Bioquest
SKU: 21187
Price: 308 USD
Availability: InStock

Protonex™ Red 780, NHS ester

Protonex™ Red 780 NHS ester is an excellent building block for developing a target-specific conjugate that can be used for monitoring a pH-sensitive biological event. Protonex™ Red 780 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 780 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 780 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 780 to become highly fluorescent and emit near-infrared light when excited by a red laser. By measuring the fluorescence intensity of Protonex™ Red 780, one can monitor the activation and trafficking of receptors or antibodies in live cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21187	1 mg	Price

References

View all 16 references: Citation Explorer

Functional coupling of apical Cl-/HCO3- exchange with CFTR in stimulated HCO3- secretion by guinea pig interlobular pancreatic duct.

Authors:

Stewart, A K and Yamamoto, A and Nakakuki, M and Kondo, T and Alper, S L and Ishiguro, H

Journal:

American journal of physiology. Gastrointestinal and liver physiology (2009): G1307-17

Hyperacidity of secreted fluid from submucosal glands in early cystic fibrosis.

Authors:

Song, Yuanlin and Salinas, Danieli and Nielson, Dennis W and Verkman, A S

Journal:

American journal of physiology. Cell physiology (2006): C741-9

Evidence against aquaporin-1-dependent CO2 permeability in lung and kidney.

Authors:

Fang, Xiaohui and Yang, Baoxue and Matthay, Michael A and Verkman, A S

Journal:

The Journal of physiology (2002): 63-9

Airway surface liquid pH in well-differentiated airway epithelial cell cultures and mouse trachea.

Authors:

Jayaraman, S and Song, Y and Verkman, A S

Journal:

American journal of physiology. Cell physiology (2001): C1504-11

Perfused rat intrahepatic bile ducts secrete and absorb water, solute, and ions.

Authors:

Masyuk, A I and Gong, A Y and Kip, S and Burke, M J and LaRusso, N F

Journal:

Gastroenterology (2000): 1672-80

Physical properties

Molecular weight	853.06
Solvent	DMSO

Spectral properties

Excitation (nm)	748
Emission (nm)	769

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 5, 2025