Protonex™ Red 780, NHS ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 853.06 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 748 |
Emission (nm) | 769 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Intracellular pH
Molecular weight 853.06 | Excitation (nm) 748 | Emission (nm) 769 |
Protonex™ Red 780 NHS ester is an excellent building block for developing a target-specific conjugate that can be used for monitoring a pH-sensitive biological event. Protonex™ Red 780 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 780 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 780 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 780 to become highly fluorescent and emit near-infrared light when excited by a red laser. By measuring the fluorescence intensity of Protonex™ Red 780, one can monitor the activation and trafficking of receptors or antibodies in live cells.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 780, NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 117.225 µL | 586.125 µL | 1.172 mL | 5.861 mL | 11.723 mL |
5 mM | 23.445 µL | 117.225 µL | 234.45 µL | 1.172 mL | 2.345 mL |
10 mM | 11.723 µL | 58.613 µL | 117.225 µL | 586.125 µL | 1.172 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 16 references: Citation Explorer
Functional coupling of apical Cl-/HCO3- exchange with CFTR in stimulated HCO3- secretion by guinea pig interlobular pancreatic duct.
Authors: Stewart, A K and Yamamoto, A and Nakakuki, M and Kondo, T and Alper, S L and Ishiguro, H
Journal: American journal of physiology. Gastrointestinal and liver physiology (2009): G1307-17
Authors: Stewart, A K and Yamamoto, A and Nakakuki, M and Kondo, T and Alper, S L and Ishiguro, H
Journal: American journal of physiology. Gastrointestinal and liver physiology (2009): G1307-17
Hyperacidity of secreted fluid from submucosal glands in early cystic fibrosis.
Authors: Song, Yuanlin and Salinas, Danieli and Nielson, Dennis W and Verkman, A S
Journal: American journal of physiology. Cell physiology (2006): C741-9
Authors: Song, Yuanlin and Salinas, Danieli and Nielson, Dennis W and Verkman, A S
Journal: American journal of physiology. Cell physiology (2006): C741-9
Evidence against aquaporin-1-dependent CO2 permeability in lung and kidney.
Authors: Fang, Xiaohui and Yang, Baoxue and Matthay, Michael A and Verkman, A S
Journal: The Journal of physiology (2002): 63-9
Authors: Fang, Xiaohui and Yang, Baoxue and Matthay, Michael A and Verkman, A S
Journal: The Journal of physiology (2002): 63-9
Airway surface liquid pH in well-differentiated airway epithelial cell cultures and mouse trachea.
Authors: Jayaraman, S and Song, Y and Verkman, A S
Journal: American journal of physiology. Cell physiology (2001): C1504-11
Authors: Jayaraman, S and Song, Y and Verkman, A S
Journal: American journal of physiology. Cell physiology (2001): C1504-11
Perfused rat intrahepatic bile ducts secrete and absorb water, solute, and ions.
Authors: Masyuk, A I and Gong, A Y and Kip, S and Burke, M J and LaRusso, N F
Journal: Gastroenterology (2000): 1672-80
Authors: Masyuk, A I and Gong, A Y and Kip, S and Burke, M J and LaRusso, N F
Journal: Gastroenterology (2000): 1672-80
Changes in cytosolic pH within Arabidopsis root columella cells play a key role in the early signaling pathway for root gravitropism.
Authors: Scott, A C and Allen, N S
Journal: Plant physiology (1999): 1291-8
Authors: Scott, A C and Allen, N S
Journal: Plant physiology (1999): 1291-8
Luminal ATP stimulates fluid and HCO3- secretion in guinea-pig pancreatic duct.
Authors: Ishiguro, H and Naruse, S and Kitagawa, M and Hayakawa, T and Case, R M and Steward, M C
Journal: The Journal of physiology (1999): 551-8
Authors: Ishiguro, H and Naruse, S and Kitagawa, M and Hayakawa, T and Case, R M and Steward, M C
Journal: The Journal of physiology (1999): 551-8
Growing pollen tubes possess a constitutive alkaline band in the clear zone and a growth-dependent acidic tip.
Authors: Feijó, J A and Sainhas, J and Hackett, G R and Kunkel, J G and Hepler, P K
Journal: The Journal of cell biology (1999): 483-96
Authors: Feijó, J A and Sainhas, J and Hackett, G R and Kunkel, J G and Hepler, P K
Journal: The Journal of cell biology (1999): 483-96
Identification of PLCgamma-dependent and -independent events during fertilization of sea urchin eggs.
Authors: Carroll, D J and Albay, D T and Terasaki, M and Jaffe, L A and Foltz, K R
Journal: Developmental biology (1999): 232-47
Authors: Carroll, D J and Albay, D T and Terasaki, M and Jaffe, L A and Foltz, K R
Journal: Developmental biology (1999): 232-47
Fluid secretion in interlobular ducts isolated from guinea-pig pancreas.
Authors: Ishiguro, H and Naruse, S and Steward, M C and Kitagawa, M and Ko, S B and Hayakawa, T and Case, R M
Journal: The Journal of physiology (1998): 407-22
Authors: Ishiguro, H and Naruse, S and Steward, M C and Kitagawa, M and Ko, S B and Hayakawa, T and Case, R M
Journal: The Journal of physiology (1998): 407-22
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Cell Preparation Considerations and Troubleshooting
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FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
AssayWise
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RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
Nucleic Acid Detection, Quantification and Imaging
Cal-520® AM, a Development Overview