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ReadiUse™ Cell Detaching Buffer

Detaching of Hela cells with ReadiUse™ Cell Detaching Buffer.
Detaching of Hela cells with ReadiUse™ Cell Detaching Buffer.
Ordering information
Price ()
Catalog Number60010
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products. It works similar to trypsin with much less toxic effect. It performs exceptionally well in detaching primary and stem cells and maintains high cell viability. ReadiUse™ cell detaching buffer is very useful for routine cell passage, analysis of cell surface markers and receptors, cell proliferation, apoptosis and flow cytometry.

Example protocol


SAMPLE EXPERIMENTAL PROTOCOL

Note:

The ReadiUse™ Cell Detaching Buffer is supplied as a sterile, ready-to use, frozen liquid. After thawing, It may be stored for up to 2 months at 4 °C.

The following protocol can be used as a guideline and can be modified as per user's need.

Sample protocol
  1. Thaw the ReadiUse™ cell detaching buffer at room temperature.

  2. Aspirate all of the media from the cell culture dish or flask. Note: It is not necessary to wash PBS.

  3. Add the ReadiUse™ cell detaching buffer to the culture dish or flask to cover the cells, typically using ~10 mL for each 75 cm2 of surface area. 

  4. Return culture to 37 °C incubator and allow cells to detach for 5–10 minutes.

  5. Count cells and passage as usual, no additional washes or enzyme inhibitors are required.

Citations


View all 30 citations: Citation Explorer
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
Authors: Chen, C. H., Chang, Y. S.
Journal: J Vis Exp (2018): se name="60010.enl" path="C:\Website\Referenc
The virtual cell based assay: Current status and future perspectives
Authors: Graepel, R., Lamon, L., Asturiol, D., Berggren, E., Joossens, E., Paini, A., Prieto, P., Whelan, M., Worth, A.
Journal: Toxicol In Vitro (2017): 258-267
Evaluation of anti-Zika virus activities of broad-spectrum antivirals and NIH clinical collection compounds using a cell-based, high-throughput screen assay
Authors: Adcock, R. S., Chu, Y. K., Golden, J. E., Chung, D. H.
Journal: Antiviral Res (2017): 47-56
A cell-based high-throughput screening assay system for inhibitor compounds of antigen presentation by HLA class II molecule
Authors: Watanabe, N., Suzuki, Y., Yonezu, T., Nakagawa, Y., Shiina, T., Hirayama, N., Inokuchi, S., Inoue, S.
Journal: Sci Rep (2017): 6798
Fluorescent Parkin Cell-Based Assay Development for the Screening of Drugs against Parkinson Disease
Authors: Villace, P., Mella, R. M., Roura-Ferrer, M., Valcarcel, M., Salado, C., Castilla, A., Kortazar, D.
Journal: SLAS Discov (2017): 67-76
Establishment of Cell-Based Neuroglobin Promoter Reporter Assay for Neuroprotective Compounds Screening
Authors: Liu, N., Yu, Z., Gao, X., Song, Y. S., Yuan, J., Xun, Y., Wang, T., Yan, F., Yuan, S., Zhang, J., Xiang, S., Lo, E. H., Wang, X.
Journal: CNS Neurol Disord Drug Targets (2016): 629-39
A cell-based screening assay to identify pharmaceutical compounds that enhance the regenerative quality of corneal repair
Authors: Gordon, G. M., LaGier, A. J., Ponchel, C., Bauskar, A., Itakura, T., Jeong, S., Patel, N., Fini, M. E.
Journal: Wound Repair Regen (2016): 89-99
Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway
Authors: Zhao, J., Shukla, S. J., Xia, M.
Journal: Methods Mol Biol (2016): 55-62
Validation of a human cell based high-throughput genotoxicity assay 'Anthem's Genotoxicity screen' using ECVAM recommended lists of genotoxic and non-genotoxic chemicals
Authors: Rajakrishna, L., Krishnan Unni, S., Subbiah, M., Sadagopan, S., Nair, A. R., Ch and rappa, R., Sambasivam, G., Sukumaran, S. K.
Journal: Toxicol In Vitro (2014): 46-53
Development of a novel cell-based assay system EPISSAY for screening epigenetic drugs and liposome formulated decitabine
Authors: Lim, S. P., Kumar, R., Akkamsetty, Y., Wang, W., Ho, K., Neilsen, P. M., Walther, D. J., Suetani, R. J., Prestidge, C., Callen, D. F.
Journal: BMC Cancer (2013): 113