AAT Bioquest

ReadiUse™ Cell Detaching Buffer

Detaching of Hela cells with ReadiUse™ Cell Detaching Buffer.
Detaching of Hela cells with ReadiUse™ Cell Detaching Buffer.
Detaching of Hela cells with ReadiUse™ Cell Detaching Buffer.
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ShippingStandard overnight for United States, inquire for international
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Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products. It works similar to trypsin with much less toxic effect. It performs exceptionally well in detaching primary and stem cells and maintains high cell viability. ReadiUse™ cell detaching buffer is very useful for routine cell passage, analysis of cell surface markers and receptors, cell proliferation, apoptosis and flow cytometry.

Example protocol



The ReadiUse™ Cell Detaching Buffer is supplied as a sterile, ready-to use, frozen liquid. After thawing, It may be stored for up to 2 months at 4 °C.

The following protocol can be used as a guideline and can be modified as per user's need.

Sample protocol
  1. Thaw the ReadiUse™ cell detaching buffer at room temperature.

  2. Aspirate all of the media from the cell culture dish or flask. Note: It is not necessary to wash PBS.

  3. Add the ReadiUse™ cell detaching buffer to the culture dish or flask to cover the cells, typically using ~10 mL for each 75 cm2 of surface area. 

  4. Return culture to 37 °C incubator and allow cells to detach for 5–10 minutes.

  5. Count cells and passage as usual, no additional washes or enzyme inhibitors are required.



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