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ReadiUse™ PCR Reaction Buffer

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Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


PCR (polymerase chain reaction) is a laboratory technique used to amplify DNA (deoxyribonucleic acid) segments. Our ready to use PCR buffer contains several components essential for the PCR reaction. The PCR reaction buffer helps to maintain a stable pH during the PCR reaction. It also contains a salt that helps to maintain the integrity of the DNA during the reaction and also enhances the activity of the DNA polymerase enzyme. MgCl2 (magnesium chloride), a cofactor, is used for maintaining the activity of the DNA polymerase enzyme.

References


View all 50 references: Citation Explorer
ONE ASSAY TO TEST THEM ALL: COMPARING MULTIPLEX ASSAYS FOR EXPANSION OF RESPIRATORY VIRUS SURVEILLANCE.
Authors: Boukli, Narjis and Flamand, Claude and Chea, Kim Lay and Heng, Leangyi and Keo, Seangmai and Sour, Kimhoung and In, Sophea and Chhim, Panha and Chhor, Bunthea and Kruy, Lomor and Feenstra, Jelena D M and Gandhi, Manoj and Okafor, Obiageli and Ulekliev, Camilla and Auerswald, Heidi and Horm, Viseth Srey and Karlsson, Erik A
Journal: medRxiv : the preprint server for health sciences (2023)
A self-developed AIM-InDel panel designated for degraded DNA analysis: Forensic feature characterization and genetic legacy investigation in the Han Chinese population.
Authors: Lan, Qiong and Li, Shuanglin and Cai, Meiming and Lei, Fanzhang and Shen, Chunmei and Zhu, Bofeng
Journal: Genomics (2023): 110620
Evaluation and clinical validation of monkeypox (mpox) virus real-time PCR assays.
Authors: Mills, Margaret G and Juergens, Kate B and Gov, Jolene P and McCormick, Carter J and Sampoleo, Reigran and Kachikis, Alisa and Amory, John K and Fang, Ferric C and Pérez-Osorio, Ailyn C and Lieberman, Nicole A P and Greninger, Alexander L
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2023): 105373
Novel and efficient method for the reconstruction of adenoviruses through isothermal assembly and its potential applications.
Authors: Wen, Ke and Resch, Matthew D and Mazboudi, Ryan and Mulhall Maasz, Hannah and Galarza, Jose M
Journal: Frontiers in medical technology (2023): 1095198
HIV-PULSE: A long-read sequencing assay for high-throughput near full-length HIV-1 proviral genome characterization.
Authors: Lambrechts, Laurens and Bonine, Noah and Verstraeten, Rita and Pardons, Marion and Noppe, Ytse and Rutsaert, Sofie and Van Nieuwerburgh, Filip and Van Criekinge, Wim and Cole, Basiel and Vandekerckhove, Linos
Journal: bioRxiv : the preprint server for biology (2023)
Association of the Mannose-Binding Lectin 2 BB Genotype with COVID-19-Related Mortality.
Authors: Kashiwagi, Yasuyo and Suzuki, Shinji and Takahashi, Ryo and Yamanaka, Gaku and Hirai, Yuji and Kawashima, Hisashi
Journal: Life (Basel, Switzerland) (2023)
An accurate genetic assay to identify human neutrophil antigen 2 deficiency.
Authors: Li, Yunfang and Schuller, Randy M and Wu, Jianming
Journal: Transfusion medicine (Oxford, England) (2023): 68-74
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Journal: Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] (2023): 443-450
A rapid multiplex assay of human malaria parasites by digital PCR.
Authors: Dong, Liu and Li, Weijia and Xu, Qianqian and Gu, Jianfei and Kang, Zhihua and Chen, Jian and Xu, Xiao and Zhang, Xinju and Zhang, Xiuming and Jiang, Haoqin and Guan, Ming
Journal: Clinica chimica acta; international journal of clinical chemistry (2023): 70-78
Effect of COP1 in Promoting the Tumorigenesis of Gastric Cancer by Down-Regulation of CDH18 via PI3K/AKT Signal Pathway.
Authors: Zhao, Benhuo and Wu, Jiaojiao and Cha, Xiuli and Mao, Guangtong and Shi, Hengliang and Fei, Sujuan and Miao, Bei
Journal: Analytical cellular pathology (Amsterdam) (2023): 5617875