ReadiUse™ Staurosporine * 1 mM DMSO stock solution*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 466.54 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Related products
| Overview |  SDSProtocol |
See also: Buffers and Lab Consumables
CAS 62996-74 | Molecular weight 466.54 |
Staurosporine is widely used as a positive control for inducing apoptosis. It has been proven that Staurosporine induces apoptosis in human malignant glioma cell lines, and arrests normal cells at the G1 checkpoint. Staurosporine is a potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases, e.g., CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiUse™ Staurosporine * 1 mM DMSO stock solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 214.344 µL | 1.072 mL | 2.143 mL | 10.717 mL | 21.434 mL |
| 5 mM | 42.869 µL | 214.344 µL | 428.688 µL | 2.143 mL | 4.287 mL |
| 10 mM | 21.434 µL | 107.172 µL | 214.344 µL | 1.072 mL | 2.143 mL |
Molarity calculator
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Product Family
| Name | Excitation (nm) | Emission (nm) |
| ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4* | 498 | 517 |
| ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free* | 565 | 574 |
Images
Figure 1. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Figure 2. Chemical structure for ReadiUse™ Staurosporine * 1 mM DMSO stock solution*.
Citations
View all 34 citations: Citation Explorer
Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62
Authors: Alsamman, K., El-Masry, O. S.
Journal: Oncol Rep (2018): 2157-2162
Authors: Alsamman, K., El-Masry, O. S.
Journal: Oncol Rep (2018): 2157-2162
Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor Cells
Authors: Lewis, G., Christiansen, L., McKenzie, J., Luo, M., Pasackow, E., Smurnyy, Y., Harrington, S., Gregory, P., Veres, G., Negre, O., Bonner, M.
Journal: Mol Ther Methods Clin Dev (2018): 313-322
Authors: Lewis, G., Christiansen, L., McKenzie, J., Luo, M., Pasackow, E., Smurnyy, Y., Harrington, S., Gregory, P., Veres, G., Negre, O., Bonner, M.
Journal: Mol Ther Methods Clin Dev (2018): 313-322
Staurosporine Derivatives Generated by Pathway Engineering in a Heterologous Host and Their Cytotoxic Selectivity
Authors: Xiao, F., Li, H., Xu, M., Li, T., Wang, J., Sun, C., Hong, K., Li, W.
Journal: J Nat Prod (2018): 1745-1751
Authors: Xiao, F., Li, H., Xu, M., Li, T., Wang, J., Sun, C., Hong, K., Li, W.
Journal: J Nat Prod (2018): 1745-1751
Talaumidin Promotes Neurite Outgrowth of Staurosporine-Differentiated RGC-5 Cells Through PI3K/Akt-Dependent Pathways
Authors: Koriyama, Y., Furukawa, A., Sugitani, K., Kubo, M., Harada, K., Fukuyama, Y.
Journal: Adv Exp Med Biol (2018): 649-653
Authors: Koriyama, Y., Furukawa, A., Sugitani, K., Kubo, M., Harada, K., Fukuyama, Y.
Journal: Adv Exp Med Biol (2018): 649-653
Staurosporine: new lease of life for parent compound of today's novel and highly successful anti-cancer drugs
Authors: Omura, S., Asami, Y., Crump, A.
Journal: J Antibiot (Tokyo) (2018): 688-701
Authors: Omura, S., Asami, Y., Crump, A.
Journal: J Antibiot (Tokyo) (2018): 688-701
Staurosporine-induced apoptotic water loss is cell- and attachment-specific
Authors: Model, M. A., Mudrak, N. J., Rana, P. S., Clements, R. J.
Journal: Apoptosis (2018): 449-455
Authors: Model, M. A., Mudrak, N. J., Rana, P. S., Clements, R. J.
Journal: Apoptosis (2018): 449-455
Staurosporine targets the Hippo pathway to inhibit cell growth
Authors: Ma, X., Li, P., Chen, P., Li, J., Huang, H., Wang, C., Li, W., Ding, J., Zhao, Y., Yu, F. X., Qi, X., Zhang, L.
Journal: J Mol Cell Biol (2018): 267-269
Authors: Ma, X., Li, P., Chen, P., Li, J., Huang, H., Wang, C., Li, W., Ding, J., Zhao, Y., Yu, F. X., Qi, X., Zhang, L.
Journal: J Mol Cell Biol (2018): 267-269
The ubiquitin E3 ligase CHIP promotes proteasomal degradation of the serine/threonine protein kinase PINK1 during staurosporine-induced cell death
Authors: Yoo, L., Chung, K. C.
Journal: J Biol Chem (2018): 1286-1297
Authors: Yoo, L., Chung, K. C.
Journal: J Biol Chem (2018): 1286-1297
Changes in the Biophysical Properties of the Cell Membrane Are Involved in the Response of Neurospora crassa to Staurosporine
Authors: Santos, F. C., Lobo, G. M., Fern and es, A. S., Videira, A., de Almeida, R. F. M.
Journal: Front Physiol (2018): 1375
Authors: Santos, F. C., Lobo, G. M., Fern and es, A. S., Videira, A., de Almeida, R. F. M.
Journal: Front Physiol (2018): 1375
Encephalitozoon cuniculi and Vittaforma corneae (Phylum Microsporidia) inhibit staurosporine-induced apoptosis in human THP-1 macrophages in vitro
Authors: Sokolova, Y. Y., Bowers, L. C., Alvarez, X., Didier, E. S.
Journal: Parasitology (2018): 1-11
Authors: Sokolova, Y. Y., Bowers, L. C., Alvarez, X., Didier, E. S.
Journal: Parasitology (2018): 1-11