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ReadiUse™ Staurosporine * 1 mM DMSO stock solution*

Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Chemical structure for ReadiUse™ Staurosporine * 1 mM DMSO stock solution*.
Ordering information
Price ()
Catalog Number80050
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight466.54
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


CAS
62996-74
Molecular weight
466.54
Staurosporine is widely used as a positive control for inducing apoptosis. It has been proven that Staurosporine induces apoptosis in human malignant glioma cell lines, and arrests normal cells at the G1 checkpoint. Staurosporine is a potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases, e.g., CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiUse™ Staurosporine * 1 mM DMSO stock solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM214.344 µL1.072 mL2.143 mL10.717 mL21.434 mL
5 mM42.869 µL214.344 µL428.688 µL2.143 mL4.287 mL
10 mM21.434 µL107.172 µL214.344 µL1.072 mL2.143 mL

Molarity calculator

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Citations


View all 34 citations: Citation Explorer
Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62
Authors: Alsamman, K., El-Masry, O. S.
Journal: Oncol Rep (2018): 2157-2162
Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor Cells
Authors: Lewis, G., Christiansen, L., McKenzie, J., Luo, M., Pasackow, E., Smurnyy, Y., Harrington, S., Gregory, P., Veres, G., Negre, O., Bonner, M.
Journal: Mol Ther Methods Clin Dev (2018): 313-322
Staurosporine Derivatives Generated by Pathway Engineering in a Heterologous Host and Their Cytotoxic Selectivity
Authors: Xiao, F., Li, H., Xu, M., Li, T., Wang, J., Sun, C., Hong, K., Li, W.
Journal: J Nat Prod (2018): 1745-1751
Talaumidin Promotes Neurite Outgrowth of Staurosporine-Differentiated RGC-5 Cells Through PI3K/Akt-Dependent Pathways
Authors: Koriyama, Y., Furukawa, A., Sugitani, K., Kubo, M., Harada, K., Fukuyama, Y.
Journal: Adv Exp Med Biol (2018): 649-653
Staurosporine: new lease of life for parent compound of today's novel and highly successful anti-cancer drugs
Authors: Omura, S., Asami, Y., Crump, A.
Journal: J Antibiot (Tokyo) (2018): 688-701
Staurosporine-induced apoptotic water loss is cell- and attachment-specific
Authors: Model, M. A., Mudrak, N. J., Rana, P. S., Clements, R. J.
Journal: Apoptosis (2018): 449-455
Staurosporine targets the Hippo pathway to inhibit cell growth
Authors: Ma, X., Li, P., Chen, P., Li, J., Huang, H., Wang, C., Li, W., Ding, J., Zhao, Y., Yu, F. X., Qi, X., Zhang, L.
Journal: J Mol Cell Biol (2018): 267-269
The ubiquitin E3 ligase CHIP promotes proteasomal degradation of the serine/threonine protein kinase PINK1 during staurosporine-induced cell death
Authors: Yoo, L., Chung, K. C.
Journal: J Biol Chem (2018): 1286-1297
Changes in the Biophysical Properties of the Cell Membrane Are Involved in the Response of Neurospora crassa to Staurosporine
Authors: Santos, F. C., Lobo, G. M., Fern and es, A. S., Videira, A., de Almeida, R. F. M.
Journal: Front Physiol (2018): 1375
Encephalitozoon cuniculi and Vittaforma corneae (Phylum Microsporidia) inhibit staurosporine-induced apoptosis in human THP-1 macrophages in vitro
Authors: Sokolova, Y. Y., Bowers, L. C., Alvarez, X., Didier, E. S.
Journal: Parasitology (2018): 1-11