Name: ReadiUse™ Staurosporine * 1 mM DMSO stock solution*
Brand: AAT Bioquest
SKU: 80050
Price: 118 USD
Availability: InStock

ReadiUse™ Staurosporine

1 mM DMSO stock solution

ReadiUse™ Staurosporine is a preformulated solution of staurosporine for inducing apoptosis and inhibiting protein kinase activity in cell-based studies.

Mechanism: Cell-permeable, reversible, ATP-competitive broad-spectrum protein kinase inhibitor.
Application: Widely used as a positive control for apoptosis induction.
Formulation: Supplied as a 1 mM DMSO stock solution.
Workflow: Example protocol uses 1 µM for 4 hours at 37 °C.
Kinase Activity: Inhibits PKC, PKA, PKG, CaM kinase, and myosin light chain kinase.

Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
80050	100 uL	Price

Overview

Staurosporine is widely used as a positive control for inducing apoptosis. It has been proven that Staurosporine induces apoptosis in human malignant glioma cell lines, and arrests normal cells at the G1 checkpoint. Staurosporine is a potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases, e.g., CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation.

Citations

View all 35 citations: Citation Explorer

Determination of the optical interference of iron oxide nanoparticles in fluorometric cytotoxicity assays

Authors:

Martin, Leisha and Lopez, Kimberly and Fritz, Shayden and Easterling, Charles P and Krawchuck, Jacob A and Poerwoprajitno, Agus R and Xu, Wei

Journal:

Heliyon (2024): e25378

Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells

Authors:

Glassmann, A., Carrillo Garcia, C., Janzen, V., Kraus, D., Veit, N., Winter, J., Probstmeier, R.

Journal:

Anal Cell Pathol (Amst) (2018): 1754085

Bioactive staurosporine derivatives from the Streptomyces sp. NB-A13

Authors:

Zhou, B., Hu, Z. J., Zhang, H. J., Li, J. Q., Ding, W. J., Ma, Z. J.

Journal:

Bioorg Chem (2018): 33-40

7-hydroxy-staurosporine, UCN-01, induces DNA damage response, and autophagy in human osteosarcoma U2-OS cells

Authors:

Lien, W. C., Chen, T. Y., Sheu, S. Y., Lin, T. C., Kang, F. C., Yu, C. H., Kuan, T. S., Huang, B. M., Wang, C. Y.

Journal:

J Cell Biochem (2018): 4729-4741

Raman spectroscopy investigation of biochemical changes in tumor spheroids with aging and after treatment with staurosporine

Authors:

Jamieson, L. E., Harrison, D. J., Campbell, C. J.

Journal:

J Biophotonics (2018): e201800201

Physical properties

Molecular weight	466.54
Solvent	DMSO

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	62996-74

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Page updated on October 28, 2026