logo
AAT Bioquest

ReadiUse™ Staurosporine * 1 mM DMSO stock solution*

Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Gallery Image 2
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight466.54
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Related products
ReadiUse™ Preactivated APC-iFluor® 700 Tandem
ReadiUse™ Preactivated APC-iFluor® 750 Tandem
ReadiUse™ Preactivated PE-iFluor® 647 Tandem
ReadiUse™ Preactivated PE-iFluor® 750 Tandem
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
ReadiUse™ Preactivated HRP NHS ester
ReadiUse™ NADPH Regenerating Kit
ReadiUse™ NADP Regenerating Kit
ReadiUse™ TCA Deproteinization Sample Preparation Kit
ReadiUse™ microscope mounting solution
ReadiUse™ 4% formaldehyde fixation solution
ReadiUse™ mammalian cell lysis buffer *5X*
ReadiUse™ probenecid, sodium salt *Water-soluble*
ReadiUse™ Preactivated PE
ReadiUse™ Preactivated APC
ReadiUse™ Preactivated PE-Cy5 Tandem
ReadiUse™ Preactivated PE-Cy5.5 Tandem
ReadiUse™ Preactivated PE-Cy7 Tandem
ReadiUse™ Preactivated PE-Texas Red Tandem
ReadiUse™ Preactivated APC-Cy5.5 Tandem
ReadiUse™ Preactivated APC-Cy7 Tandem
ReadiUse™ TCEP removal solution
ReadiUse™ WST-8 *50 mM aqueous solution*
ReadiUse™ Rapid Luminometric ATP Assay Kit
ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*
ReadiUse™ Preactivated PE-iFluor® 594 Tandem
ReadiUse™ Cell Detaching Buffer
ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
ReadiUse™ Bio-Gel P-6 spin column
ReadiUse™ bacterial cell lysis buffer *5X*
ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*
ReadiUse™ Preactivated PE-iFluor® 700 Tandem
ReadiUse™ dNTP Mix *10 mM*
ReadiUse™ Viral RNA Lysis Buffer
ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer
ReadiUse™ Preactivated PerCP
ReadiUse™ Preactivated PerCP-Cy5.5 Tandem
ReadiUse™ Preactivated PE-iFluor® 660 Tandem
ReadiUse™ 10KD Spin Filter
ReadiUse™ Preactivated APC-iFluor® 800 Tandem
ReadiUse™ 1 Kb Plus DNA Ladder
ReadiUse™ GeneRuler 1 kb DNA Ladder
ReadiUse™ 100 bp DNA Ladder
ReadiUse™ ddNTP Terminator Mix *10 mM*
ReadiUse™ dNTP Mix Set *10 mM PCR Grade*
ReadiUse™ Preactivated HRP maleimide
ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
ReadiUse™ Disposable PD-10 Desalting Column
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin]
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin]
ReadiUse™ 6-Color Human TBNK Antibody Kit *Dry Reagent Format*
ReadiUse™ MTS Reagent *5 mM aqueous solution*
ReadiUse™ Preactivated PE-Texas Red Maleimide
ReadiUse™ Preactivated PE-Cy5 Maleimide
ReadiUse™ Preactivated PE-Cy5.5 Maleimide
ReadiUse™ Preactivated PE-Cy7 Maleimide
ReadiUse™ Preactivated APC-AF700 Maleimide
ReadiUse™ Preactivated APC-Cy5.5 Maleimide
ReadiUse™ Preactivated APC-Cy7 Maleimide
ReadiUse™ Preactivated PerCP Maleimide
ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide
ReadiUse™ Preactivated PerCP-Cy7 Maleimide
ReadiUse™ PCR Reaction Buffer
ReadiUse™ Universal Kinase Reaction Stopping Buffer
ReadiUse™ Yeast Cell Lysis Buffer *5X*
Show More (57)

OverviewpdfSDSpdfProtocol


CAS
62996-74
Molecular weight
466.54
Staurosporine is widely used as a positive control for inducing apoptosis. It has been proven that Staurosporine induces apoptosis in human malignant glioma cell lines, and arrests normal cells at the G1 checkpoint. Staurosporine is a potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases, e.g., CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiUse™ Staurosporine * 1 mM DMSO stock solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM214.344 µL1.072 mL2.143 mL10.717 mL21.434 mL
5 mM42.869 µL214.344 µL428.688 µL2.143 mL4.287 mL
10 mM21.434 µL107.172 µL214.344 µL1.072 mL2.143 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Images


Citations


View all 35 citations: Citation Explorer
Determination of the optical interference of iron oxide nanoparticles in fluorometric cytotoxicity assays
Authors: Martin, Leisha and Lopez, Kimberly and Fritz, Shayden and Easterling, Charles P and Krawchuck, Jacob A and Poerwoprajitno, Agus R and Xu, Wei
Journal: Heliyon (2024): e25378
Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62
Authors: Alsamman, K., El-Masry, O. S.
Journal: Oncol Rep (2018): 2157-2162
Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor Cells
Authors: Lewis, G., Christiansen, L., McKenzie, J., Luo, M., Pasackow, E., Smurnyy, Y., Harrington, S., Gregory, P., Veres, G., Negre, O., Bonner, M.
Journal: Mol Ther Methods Clin Dev (2018): 313-322
Staurosporine Derivatives Generated by Pathway Engineering in a Heterologous Host and Their Cytotoxic Selectivity
Authors: Xiao, F., Li, H., Xu, M., Li, T., Wang, J., Sun, C., Hong, K., Li, W.
Journal: J Nat Prod (2018): 1745-1751
Talaumidin Promotes Neurite Outgrowth of Staurosporine-Differentiated RGC-5 Cells Through PI3K/Akt-Dependent Pathways
Authors: Koriyama, Y., Furukawa, A., Sugitani, K., Kubo, M., Harada, K., Fukuyama, Y.
Journal: Adv Exp Med Biol (2018): 649-653
Staurosporine: new lease of life for parent compound of today&apos;s novel and highly successful anti-cancer drugs
Authors: Omura, S., Asami, Y., Crump, A.
Journal: J Antibiot (Tokyo) (2018): 688-701
Staurosporine-induced apoptotic water loss is cell- and attachment-specific
Authors: Model, M. A., Mudrak, N. J., Rana, P. S., Clements, R. J.
Journal: Apoptosis (2018): 449-455
Staurosporine targets the Hippo pathway to inhibit cell growth
Authors: Ma, X., Li, P., Chen, P., Li, J., Huang, H., Wang, C., Li, W., Ding, J., Zhao, Y., Yu, F. X., Qi, X., Zhang, L.
Journal: J Mol Cell Biol (2018): 267-269
The ubiquitin E3 ligase CHIP promotes proteasomal degradation of the serine/threonine protein kinase PINK1 during staurosporine-induced cell death
Authors: Yoo, L., Chung, K. C.
Journal: J Biol Chem (2018): 1286-1297
Changes in the Biophysical Properties of the Cell Membrane Are Involved in the Response of Neurospora crassa to Staurosporine
Authors: Santos, F. C., Lobo, G. M., Fern and es, A. S., Videira, A., de Almeida, R. F. M.
Journal: Front Physiol (2018): 1375