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ReadiUse™ Staurosporine * 1 mM DMSO stock solution*

Staurosporine is widely used as a positive control for inducing apoptosis. It has been proven that Staurosporine induces apoptosis in human malignant glioma cell lines, and arrests normal cells at the G1 checkpoint. Staurosporine is a potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases, e.g., CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation.

Citations

View all 35 citations: Citation Explorer
Determination of the optical interference of iron oxide nanoparticles in fluorometric cytotoxicity assays
Authors: Martin, Leisha and Lopez, Kimberly and Fritz, Shayden and Easterling, Charles P and Krawchuck, Jacob A and Poerwoprajitno, Agus R and Xu, Wei
Journal: Heliyon (2024): e25378
Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells
Authors: Glassmann, A., Carrillo Garcia, C., Janzen, V., Kraus, D., Veit, N., Winter, J., Probstmeier, R.
Journal: Anal Cell Pathol (Amst) (2018): 1754085
Bioactive staurosporine derivatives from the Streptomyces sp. NB-A13
Authors: Zhou, B., Hu, Z. J., Zhang, H. J., Li, J. Q., Ding, W. J., Ma, Z. J.
Journal: Bioorg Chem (2018): 33-40
7-hydroxy-staurosporine, UCN-01, induces DNA damage response, and autophagy in human osteosarcoma U2-OS cells
Authors: Lien, W. C., Chen, T. Y., Sheu, S. Y., Lin, T. C., Kang, F. C., Yu, C. H., Kuan, T. S., Huang, B. M., Wang, C. Y.
Journal: J Cell Biochem (2018): 4729-4741
Raman spectroscopy investigation of biochemical changes in tumor spheroids with aging and after treatment with staurosporine
Authors: Jamieson, L. E., Harrison, D. J., Campbell, C. J.
Journal: J Biophotonics (2018): e201800201
Page updated on October 11, 2024

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Catalog Number80050
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Physical properties

Molecular weight

466.54

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

62996-74
Jurkat cells were treated without (Green) or with 1 &mu;M staurosporine (Red) in 37 &ordm;C for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Jurkat cells were treated without (Green) or with 1 &mu;M staurosporine (Red) in 37 &ordm;C for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Jurkat cells were treated without (Green) or with 1 &mu;M staurosporine (Red) in 37 &ordm;C for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
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