Name: ReadiUse™ WST-8 *50 mM aqueous solution*
Brand: AAT Bioquest
SKU: 15705
Availability: InStock

ReadiUse™ WST-8

50 mM aqueous solution

ReadiUse™ WST-8 *50 mM aqueous solution* is a colorimetric cell viability reagent for assessing cellular metabolic activity in proliferation and cytotoxicity assays.

Detection Principle: Cellular oxidoreductases reduce WST-8 to a water-soluble orange formazan dye.
Signal Readout: Formazan product has an absorbance maximum at 460 nm. AAT Bioquest
Sensitivity: Provides higher detection sensitivity than MTT, XTT, or MTS tetrazolium assays.
Cell Compatibility: Low cytotoxicity supports extended incubations of 24–48 hours.
Applications: Suitable for cell viability, proliferation, and cytotoxicity assays.

Chemical structure for ReadiUse™ WST-8 *50 mM aqueous solution*

Datasheet

Catalog	Size	Price
15705	25 mg	Price
15706	100 mg	Price
15707	1 g	Price

Overview

WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor, 1-methoxy phenazine methosulfate, NADPH-dependent cellular oxidoreductases, acting via plasma membrane electron transport, reduce the colorless cell-impermeant WST-8 outside the cell to a water-soluble orange formazan dye with absorbance max at 460 nm. WST-8 is very stable, and tolerates a long time incubation (such as 24 to 48 hours) due to its little cytotoxicity. WST-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS. It is typically used as a cell viability indicator in cell proliferation assays.

Citations

View all 6 citations: Citation Explorer

Establishment, culture and characterization of gemcitabine hydrochloride-resistant human non-small cell lung carcinoma cell line derived cancer stem cells

Authors:

Demir Cevizlidere, Bahar and Uysal, Onur and Avci, Huseyin and Gunes Bagis, Sibel and Semerci Sevimli, Tugba and Dincer, Murat and Qomi Ekenel, Emilia and Kara, Suleyman Gokhan and Soykan, Merve Nur and Eker Sariboyaci, Ayla

Journal:

Cell Biochemistry and Function (2024): e4079

Cytotoxicity effect and transcriptome analysis of PCV3-infected cells revealed potential viral pathogenic mechanisms

Authors:

Chang, Chia-Chun and Wu, Ching-Ying and Wu, Chi-Ming and Wu, Ching-Wei and Wang, Yi-Chen and Lin, Guang-Jan and Chien, Maw-Sheng and Huang, Chienjin

Journal:

Microbial Pathogenesis (2024): 106715

Exploring the surface epitope and nuclear localization analysis of porcine circovirus type 3 capsid protein

Authors:

Chang, Chia-Chun and Wu, Ching-Ying and Ciou, Jhao-Guan and Wu, Ching-Wei and Wang, Yi-Chen and Chang, Hui-Wen and Chien, Maw-Sheng and Huang, Chienjin

Journal:

AMB Express (2023): 1--11

miR-218 inhibits the proliferation of human glioma cells through downregulation of Yin Yang 1

Authors:

Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng

Journal:

Molecular Medicine Reports (2018): 1926--1932

MiR-218 suppresses osteosarcoma proliferation by down-regulating BMI1

Authors:

Xuan, Chengmin and Jin, Mingwei and Gao, Yong and Xu, Shumei and Wang, Lei and Wang, Yuan and Han, Rui and An, Qi

Journal:

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2017): 6342--6350

References

View all 5 references: Citation Explorer

A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line

Authors:

Tiwari, K.; Wavdhane, M.; Haque, S.; Govender, T.; Kruger, H. G.; Mishra, M. K.; Ch and ra, R.; Tiwari, D.

Journal:

Pharm Biol (2015): 849-54

Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria

Authors:

Tsukatani, T.; Suenaga, H.; Shiga, M.; Noguchi, K.; Ishiyama, M.; Ezoe, T.; Matsumoto, K.

Journal:

J Microbiol Methods (2012): 160-6

WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells

Authors:

Stoddart, M. J.

Journal:

Methods Mol Biol (2011): 21-5

monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates

Authors:

Brady, A. J.; Kearney, P.; Tunney, M. M., Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium

Journal:

J Microbiol Methods (2007): 305-11

Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency

Authors:

Arai, M.; Kosuge, K.; Kawamoto, F.; Matsuoka, H.

Journal:

Acta Med Okayama (2006): 127-34

Physical properties

Molecular weight	600.5
Solvent	Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	193149-74-5

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Page updated on October 12, 2026