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Rhod-4™ alkyne

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Physical properties
Molecular weight852.85
Spectral properties
Excitation (nm)523
Emission (nm)551
Quantum yield0.11
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Quantum yield
Rhod-4™ alkyne is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to azido-containing biomolecules via the well-known click chemistry. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM117.254 µL586.27 µL1.173 mL5.863 mL11.725 mL
5 mM23.451 µL117.254 µL234.508 µL1.173 mL2.345 mL
10 mM11.725 µL58.627 µL117.254 µL586.27 µL1.173 mL

Molarity calculator

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Spectral properties

Excitation (nm)523
Emission (nm)551
Quantum yield0.11

Product Family

NameExcitation (nm)Emission (nm)Quantum yield
Rhod-4™, AM5235510.11



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