Rhod-4™ alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 852.85 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 523 |
Emission (nm) | 551 |
Quantum yield | 0.11 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
Rhod-4™ amine |
Rhod-4™ maleimide |
Rhod-4™ azide |
Related products
Rhod-4™, sodium salt |
Rhod-4™, potassium salt |
Overview | SDSProtocol |
Molecular weight 852.85 | Excitation (nm) 523 | Emission (nm) 551 | Quantum yield 0.11 |
Rhod-4™ alkyne is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to azido-containing biomolecules via the well-known click chemistry. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 117.254 µL | 586.27 µL | 1.173 mL | 5.863 mL | 11.725 mL |
5 mM | 23.451 µL | 117.254 µL | 234.508 µL | 1.173 mL | 2.345 mL |
10 mM | 11.725 µL | 58.627 µL | 117.254 µL | 586.27 µL | 1.173 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Excitation (nm) | 523 |
Emission (nm) | 551 |
Quantum yield | 0.11 |
Images
References
View all 50 references: Citation Explorer
Mitochondria-Endoplasmic Reticulum Contact Sites Dynamics and Calcium Homeostasis Are Differentially Disrupted in PINK1-PD or PRKN-PD Neurons.
Authors: Grossmann, Dajana and Malburg, Nina and Glaß, Hannes and Weeren, Veronika and Sondermann, Verena and Pfeiffer, Julia F and Petters, Janine and Lukas, Jan and Seibler, Philip and Klein, Christine and Grünewald, Anne and Hermann, Andreas
Journal: Movement disorders : official journal of the Movement Disorder Society (2023)
Authors: Grossmann, Dajana and Malburg, Nina and Glaß, Hannes and Weeren, Veronika and Sondermann, Verena and Pfeiffer, Julia F and Petters, Janine and Lukas, Jan and Seibler, Philip and Klein, Christine and Grünewald, Anne and Hermann, Andreas
Journal: Movement disorders : official journal of the Movement Disorder Society (2023)
Palmitic acid methyl ester induces cardiac hypertrophy through activating the GPR receptor-mediated changes of intracellular calcium concentrations and mitochondrial functions.
Authors: Lien, Chih-Feng and Chiu, Hung-Wen and Lee, Wen-Sen and Lin, Jian-Hong and Wang, Yi-Shun and Ting, Pei-Ching and Luo, Yu-Po and Chang, Jui-Chih and Yang, Kun-Ta
Journal: Journal of cellular physiology (2023): 242-256
Authors: Lien, Chih-Feng and Chiu, Hung-Wen and Lee, Wen-Sen and Lin, Jian-Hong and Wang, Yi-Shun and Ting, Pei-Ching and Luo, Yu-Po and Chang, Jui-Chih and Yang, Kun-Ta
Journal: Journal of cellular physiology (2023): 242-256
Cisplatin induces damage of auditory cells: Possible relation with dynamic variation in calcium homeostasis and responding channels.
Authors: Zhao, Hao and Xu, Yue and Song, Xinlei and Zhang, Qingchen and Wang, Yajie and Yin, Haiyan and Bai, Xiaohui and Li, Jianfeng
Journal: European journal of pharmacology (2022): 174662
Authors: Zhao, Hao and Xu, Yue and Song, Xinlei and Zhang, Qingchen and Wang, Yajie and Yin, Haiyan and Bai, Xiaohui and Li, Jianfeng
Journal: European journal of pharmacology (2022): 174662
Inhibitory Effects of 6,8-Diprenylorobol on Endometriosis Progression in Humans by Disrupting Calcium Homeostasis and Mitochondrial Function.
Authors: Song, Jisoo and Song, Gwonhwa and Park, Sunwoo and Lim, Whasun
Journal: Antioxidants (Basel, Switzerland) (2022)
Authors: Song, Jisoo and Song, Gwonhwa and Park, Sunwoo and Lim, Whasun
Journal: Antioxidants (Basel, Switzerland) (2022)
NiONPs-induced alteration in calcium signaling and mitochondrial function in pulmonary artery endothelial cells involves oxidative stress and TRPV4 channels disruption.
Authors: Germande, Ophélie and Baudrimont, Magalie and Beaufils, Fabien and Freund-Michel, Véronique and Ducret, Thomas and Quignard, Jean-François and Errera, Marie-Hélène and Lacomme, Sabrina and Gontier, Etienne and Mornet, Stéphane and Bejko, Megi and Muller, Bernard and Marthan, Roger and Guibert, Christelle and Deweirdt, Juliette and Baudrimont, Isabelle
Journal: Nanotoxicology (2022): 29-51
Authors: Germande, Ophélie and Baudrimont, Magalie and Beaufils, Fabien and Freund-Michel, Véronique and Ducret, Thomas and Quignard, Jean-François and Errera, Marie-Hélène and Lacomme, Sabrina and Gontier, Etienne and Mornet, Stéphane and Bejko, Megi and Muller, Bernard and Marthan, Roger and Guibert, Christelle and Deweirdt, Juliette and Baudrimont, Isabelle
Journal: Nanotoxicology (2022): 29-51
The Effect of Primary Root Canal Treatment on Dentinal Tubule Penetration of Calcium Silicate-based Sealers during Endodontic Retreatment.
Authors: Eğemen, Ayşenur and Belli, Sema
Journal: Journal of endodontics (2022): 1169-1177
Authors: Eğemen, Ayşenur and Belli, Sema
Journal: Journal of endodontics (2022): 1169-1177
Decabromodiphenyl ether initiates mitochondria-dependent apoptosis by disrupting calcium homeostasis in mice livers.
Authors: Che, Siyan and Chen, Sunni and Li, Shiqi and Ruan, Zheng
Journal: Chemosphere (2022): 132767
Authors: Che, Siyan and Chen, Sunni and Li, Shiqi and Ruan, Zheng
Journal: Chemosphere (2022): 132767
Whole brain irradiation in mice causes long-term impairment in astrocytic calcium signaling but preserves astrocyte-astrocyte coupling.
Authors: Institoris, Adam and Murphy-Royal, Ciaran and Tarantini, Stefano and Yabluchanskiy, Andriy and Haidey, Jordan N and Csiszar, Anna and Ungvari, Zoltan and Gordon, Grant R
Journal: GeroScience (2021): 197-212
Authors: Institoris, Adam and Murphy-Royal, Ciaran and Tarantini, Stefano and Yabluchanskiy, Andriy and Haidey, Jordan N and Csiszar, Anna and Ungvari, Zoltan and Gordon, Grant R
Journal: GeroScience (2021): 197-212
Visualization of Dynamic Mitochondrial Calcium Fluxes in Isolated Cardiomyocytes.
Authors: Krstic, Anna Maria and Power, Amelia Sally and Ward, Marie-Louise
Journal: Frontiers in physiology (2021): 808798
Authors: Krstic, Anna Maria and Power, Amelia Sally and Ward, Marie-Louise
Journal: Frontiers in physiology (2021): 808798
Local recovery of cardiac calcium-induced calcium release interrogated by ultra-effective, two-photon uncaging of calcium.
Authors: Janicek, Radoslav and Agarwal, Hitesh and Gómez, Ana M and Egger, Marcel and Ellis-Davies, Graham C R and Niggli, Ernst
Journal: The Journal of physiology (2021): 3841-3852
Authors: Janicek, Radoslav and Agarwal, Hitesh and Gómez, Ana M and Egger, Marcel and Ellis-Davies, Graham C R and Niggli, Ernst
Journal: The Journal of physiology (2021): 3841-3852