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Rhod-4™ maleimide

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Physical properties
Molecular weight893.86
Spectral properties
Excitation (nm)523
Emission (nm)551
Quantum yield0.11
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Quantum yield
Rhod-4™ maleimide is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be selectively conjugated to thiol-containing biomolecules such as antibodies, thiol-modified oligos, or peptides containing a cysteine residue. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM111.874 µL559.372 µL1.119 mL5.594 mL11.187 mL
5 mM22.375 µL111.874 µL223.749 µL1.119 mL2.237 mL
10 mM11.187 µL55.937 µL111.874 µL559.372 µL1.119 mL

Molarity calculator

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Spectral properties

Excitation (nm)523
Emission (nm)551
Quantum yield0.11

Product Family

NameExcitation (nm)Emission (nm)Quantum yield
Rhod-4™, AM5235510.11



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