Rhod-4™ maleimide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	893.86
Solvent	DMSO

Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Overview SDS Protocol

Molecular weight

893.86

Excitation (nm)

523

Emission (nm)

551

Quantum yield

0.1¹

Rhod-4™ maleimide is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be selectively conjugated to thiol-containing biomolecules such as antibodies, thiol-modified oligos, or peptides containing a cysteine residue. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	111.874 µL	559.372 µL	1.119 mL	5.594 mL	11.187 mL
5 mM	22.375 µL	111.874 µL	223.749 µL	1.119 mL	2.237 mL
10 mM	11.187 µL	55.937 µL	111.874 µL	559.372 µL	1.119 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Rhod-4™, AM	523	551	0.1¹

Images

Figure 1. Rhod-4™ maleimide is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be selectively conjugated to thiol-containing biomolecules such as antibodies, thiol-modified oligos or peptides containing a cysteine residue.

References

View all 50 references: Citation Explorer

Mitochondria-Endoplasmic Reticulum Contact Sites Dynamics and Calcium Homeostasis Are Differentially Disrupted in PINK1-PD or PRKN-PD Neurons.
Authors: Grossmann, Dajana and Malburg, Nina and Glaß, Hannes and Weeren, Veronika and Sondermann, Verena and Pfeiffer, Julia F and Petters, Janine and Lukas, Jan and Seibler, Philip and Klein, Christine and Grünewald, Anne and Hermann, Andreas
Journal: Movement disorders : official journal of the Movement Disorder Society (2023)

Cardiomyocyte Targeting Peptide to Deliver Amiodarone.
Authors: Zahid, Maliha and Weber, Beth and Yurko, Ray and Islam, Kazi and Agrawal, Vaishavi and Lopuszynski, Jack and Yagi, Hisato and Salama, Guy
Journal: bioRxiv : the preprint server for biology (2023)

The Effect of Primary Root Canal Treatment on Dentinal Tubule Penetration of Calcium Silicate-based Sealers during Endodontic Retreatment.
Authors: Eğemen, Ayşenur and Belli, Sema
Journal: Journal of endodontics (2022): 1169-1177

Dual SGLT-1 and SGLT-2 inhibition improves left atrial dysfunction in HFpEF.
Authors: Bode, David and Semmler, Lukas and Wakula, Paulina and Hegemann, Niklas and Primessnig, Uwe and Beindorff, Nicola and Powell, David and Dahmen, Raphael and Ruetten, Hartmut and Oeing, Christian and Alogna, Alessio and Messroghli, Daniel and Pieske, Burkert M and Heinzel, Frank R and Hohendanner, Felix
Journal: Cardiovascular diabetology (2021): 7

Whole brain irradiation in mice causes long-term impairment in astrocytic calcium signaling but preserves astrocyte-astrocyte coupling.
Authors: Institoris, Adam and Murphy-Royal, Ciaran and Tarantini, Stefano and Yabluchanskiy, Andriy and Haidey, Jordan N and Csiszar, Anna and Ungvari, Zoltan and Gordon, Grant R
Journal: GeroScience (2021): 197-212

KairoSight: Open-Source Software for the Analysis of Cardiac Optical Data Collected From Multiple Species.
Authors: Cooper, Blake L and Gloschat, Chris and Swift, Luther M and Prudencio, Tomas and McCullough, Damon and Jaimes, Rafael and Posnack, Nikki G
Journal: Frontiers in physiology (2021): 752940

Cardiomyocyte depolarization triggers NOS-dependent NO transient after calcium release, reducing the subsequent calcium transient.
Authors: Mosqueira, Matias and Konietzny, Roland and Andresen, Carolin and Wang, Chao and H A Fink, Rainer
Journal: Basic research in cardiology (2021): 18

Preserved Ca2+ handling and excitation-contraction coupling in muscle fibres from diet-induced obese mice.
Authors: Jaque-Fernandez, Francisco and Beaulant, Agathe and Berthier, Christine and Monteiro, Laloé and Allard, Bruno and Casas, Mariana and Rieusset, Jennifer and Jacquemond, Vincent
Journal: Diabetologia (2020): 2471-2481

Ruthenium red improves blastocyst developmental competence by regulating mitochondrial Ca2+ and mitochondrial functions in fertilized porcine oocytes in vitro.
Authors: Jegal, Ho-Geun and Park, Hyo-Jin and Kim, Jin-Woo and Yang, Seul-Gi and Kim, Min-Ji and Koo, Deog-Bon
Journal: The Journal of reproduction and development (2020): 377-386

Generalized ratiometric fluorescence nanosensors based on carbon dots and an advanced chemometric model.
Authors: Yan, Xiu-Fang and Chen, Zeng-Ping and Huang, Ying and Kang, Chao and Yu, Ru-Qin
Journal: Talanta (2019): 233-240