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Rhod-4™ amine

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Physical properties
Molecular weight856.76
Spectral properties
Excitation (nm)523
Emission (nm)551
Quantum yield0.11
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Quantum yield
Rhod-4™ amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to carboxy-containing biomolecules via activated esters or EDC chemistry. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM116.719 µL583.594 µL1.167 mL5.836 mL11.672 mL
5 mM23.344 µL116.719 µL233.438 µL1.167 mL2.334 mL
10 mM11.672 µL58.359 µL116.719 µL583.594 µL1.167 mL

Molarity calculator

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Spectral properties

Excitation (nm)523
Emission (nm)551
Quantum yield0.11

Product Family

NameExcitation (nm)Emission (nm)Quantum yield
Rhod-4™, AM5235510.11



View all 6 references: Citation Explorer
Dexamethasone Treatment Increases the Intracellular Calcium Level Through TRPV6 in A549 Cells.
Authors: Jeon, Bo-Hui and Yoo, Yeong-Min and Jung, Eui-Man and Jeung, Eui-Bae
Journal: International journal of molecular sciences (2020)
Cigarette smoke extraxt influences intracellular calcium concentration in A549 cells.
Authors: Yoo, Y-M and Jung, E-M and Jeon, B-H and Tran, D N and Jeung, E-B
Journal: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (2020)
A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells.
Authors: Lock, Jeffrey T and Parker, Ian and Smith, Ian F
Journal: Cell calcium (2015): 638-48
Analysis of normal and rcdl Irish setter retinal proteins.
Authors: Cunnick, J and Takemoto, D
Journal: Progress in clinical and biological research (1989): 441-54
Rod/cone dysplasia in Irish setters. Presence of an altered rhodopsin.
Authors: Cunnick, J and Rider, M and Takemoto, L J and Takemoto, D J
Journal: The Biochemical journal (1988): 335-41
Antisera to synthetic peptides of bovine rhodopsin: use as site-specific probes of disc membrane changes in retinal dystrophic dogs.
Authors: Takemoto, D J and Spooner, B and Takemoto, L J
Journal: Biochemical and biophysical research communications (1985): 438-44