Rhod-4™ amine

Rhod-4™ amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to carboxy-containing biomolecules via activated esters or EDC chemistry. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21124	100 ug	Price

References

View all 6 references: Citation Explorer

Dexamethasone Treatment Increases the Intracellular Calcium Level Through TRPV6 in A549 Cells.

Authors:

Jeon, Bo-Hui and Yoo, Yeong-Min and Jung, Eui-Man and Jeung, Eui-Bae

Journal:

International journal of molecular sciences (2020)

Cigarette smoke extraxt influences intracellular calcium concentration in A549 cells.

Authors:

Yoo, Y-M and Jung, E-M and Jeon, B-H and Tran, D N and Jeung, E-B

Journal:

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (2020)

A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells.

Authors:

Lock, Jeffrey T and Parker, Ian and Smith, Ian F

Journal:

Cell calcium (2015): 638-48

Analysis of normal and rcdl Irish setter retinal proteins.

Authors:

Cunnick, J and Takemoto, D

Journal:

Progress in clinical and biological research (1989): 441-54

Rod/cone dysplasia in Irish setters. Presence of an altered rhodopsin.

Authors:

Cunnick, J and Rider, M and Takemoto, L J and Takemoto, D J

Journal:

The Biochemical journal (1988): 335-41

Physical properties

Molecular weight	1046.32
Solvent	DMSO

Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 31, 2025