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Rhodamine aldehyde [5-TAMRA aldehyde]

Rhodamine aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated rhodamine dye can be easily detected by a common fluorescence instrument under TRITC or Cy3 channel.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
Rhodamine 800 *CAS 137993-41-0*682704--
Rhodamine B *CAS 81-88-9*54656710600010.71, 0.432
Rhodamine 6G *CAS 989-38-8*52554811600010.951
Rhodamine 110 *CAS 13558-31-1*50052280000-
Rhodamine 123 *CAS 62669-70-9*50852885200-
Fluorescein aldehyde [5-FAM aldehyde]49351783000-
Cy3 aldehyde55556915000010.151
Cy5 aldehyde65167025000010.271, 0.42
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*499520710000.921

Citations

View all 1 citations: Citation Explorer
Transient Competitors to Modulate Dynamic Covalent Cross-Linking of Recombinant Hydrogels
Authors: Gilchrist, Aidan E and Liu, Yueming and Klett, Katarina and Liu, Yu-Chung and Ceva, Sof{\'\i}a and Heilshorn, Sarah C
Journal: Chemistry of Materials (2023)

References

View all 50 references: Citation Explorer
Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982
Super-Resolution Fluorescence Imaging of Arabidopsis thaliana Transfer Cell Wall Ingrowths using Pseudo-Schiff Labelling Adapted for the Use of Different Dyes.
Authors: Rae, Angus E and Wei, Xiaoyang and Flores-Rodriguez, Neftali and McCurdy, David W and Collings, David A
Journal: Plant & cell physiology (2020): 1775-1787
A novel rhodamine-based colorimetric and fluorometric probe for simultaneous detection of multi-metal ions.
Authors: Zhang, Yuqi and Wang, Wang and Li, Ran and Zhang, Ensheng and Li, Zhonghua and Tang, Long and Han, Bo and Hou, Xiufang and Wang, Ji-Jiang
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118050
A rhodamine-based turn-on nitric oxide sensor in aqueous medium with endogenous cell imaging: an unusual formation of nitrosohydroxylamine.
Authors: Alam, Rabiul and Islam, Abu Saleh Musha and Sasmal, Mihir and Katarkar, Atul and Ali, Mahammad
Journal: Organic & biomolecular chemistry (2018): 3910-3920
Fluorescent probes for detecting cysteine.
Authors: Yan, Fanyong and Sun, Xiaodong and Zu, Fanlin and Bai, Zhangjun and Jiang, Yingxia and Fan, Keqing and Wang, Jie
Journal: Methods and applications in fluorescence (2018): 042001
Page updated on November 3, 2024

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Physical properties

Molecular weight

536.03

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

552

Emission (nm)

578

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501