Rhodamine aldehyde [5-TAMRA aldehyde]
Rhodamine aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated rhodamine dye can be easily detected by a common fluorescence instrument under TRITC or Cy3 channel.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Rhodamine 800 *CAS 137993-41-0* | 682 | 704 | - | - |
Rhodamine B *CAS 81-88-9* | 546 | 567 | 1060001 | 0.71, 0.432 |
Rhodamine 6G *CAS 989-38-8* | 525 | 548 | 1160001 | 0.951 |
Rhodamine 110 *CAS 13558-31-1* | 500 | 522 | 80000 | - |
Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 | - |
Fluorescein aldehyde [5-FAM aldehyde] | 493 | 517 | 83000 | - |
Cy3 aldehyde | 555 | 569 | 1500001 | 0.151 |
Cy5 aldehyde | 651 | 670 | 2500001 | 0.271, 0.42 |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 |
Citations
View all 1 citations: Citation Explorer
Transient Competitors to Modulate Dynamic Covalent Cross-Linking of Recombinant Hydrogels
Authors: Gilchrist, Aidan E and Liu, Yueming and Klett, Katarina and Liu, Yu-Chung and Ceva, Sof{\'\i}a and Heilshorn, Sarah C
Journal: Chemistry of Materials (2023)
Authors: Gilchrist, Aidan E and Liu, Yueming and Klett, Katarina and Liu, Yu-Chung and Ceva, Sof{\'\i}a and Heilshorn, Sarah C
Journal: Chemistry of Materials (2023)
References
View all 50 references: Citation Explorer
Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982
Super-Resolution Fluorescence Imaging of Arabidopsis thaliana Transfer Cell Wall Ingrowths using Pseudo-Schiff Labelling Adapted for the Use of Different Dyes.
Authors: Rae, Angus E and Wei, Xiaoyang and Flores-Rodriguez, Neftali and McCurdy, David W and Collings, David A
Journal: Plant & cell physiology (2020): 1775-1787
Authors: Rae, Angus E and Wei, Xiaoyang and Flores-Rodriguez, Neftali and McCurdy, David W and Collings, David A
Journal: Plant & cell physiology (2020): 1775-1787
A novel rhodamine-based colorimetric and fluorometric probe for simultaneous detection of multi-metal ions.
Authors: Zhang, Yuqi and Wang, Wang and Li, Ran and Zhang, Ensheng and Li, Zhonghua and Tang, Long and Han, Bo and Hou, Xiufang and Wang, Ji-Jiang
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118050
Authors: Zhang, Yuqi and Wang, Wang and Li, Ran and Zhang, Ensheng and Li, Zhonghua and Tang, Long and Han, Bo and Hou, Xiufang and Wang, Ji-Jiang
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118050
A rhodamine-based turn-on nitric oxide sensor in aqueous medium with endogenous cell imaging: an unusual formation of nitrosohydroxylamine.
Authors: Alam, Rabiul and Islam, Abu Saleh Musha and Sasmal, Mihir and Katarkar, Atul and Ali, Mahammad
Journal: Organic & biomolecular chemistry (2018): 3910-3920
Authors: Alam, Rabiul and Islam, Abu Saleh Musha and Sasmal, Mihir and Katarkar, Atul and Ali, Mahammad
Journal: Organic & biomolecular chemistry (2018): 3910-3920
Fluorescent probes for detecting cysteine.
Authors: Yan, Fanyong and Sun, Xiaodong and Zu, Fanlin and Bai, Zhangjun and Jiang, Yingxia and Fan, Keqing and Wang, Jie
Journal: Methods and applications in fluorescence (2018): 042001
Authors: Yan, Fanyong and Sun, Xiaodong and Zu, Fanlin and Bai, Zhangjun and Jiang, Yingxia and Fan, Keqing and Wang, Jie
Journal: Methods and applications in fluorescence (2018): 042001
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