Rhodamine aldehyde [5-TAMRA aldehyde]
Ordering information
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Additional ordering information
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Physical properties
Molecular weight | 536.03 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
Molecular weight 536.03 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 552 | Emission (nm) 578 |
Rhodamine aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated rhodamine dye can be easily detected by a common fluorescence instrument under TRITC or Cy3 channel.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine aldehyde [5-TAMRA aldehyde] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 186.557 µL | 932.784 µL | 1.866 mL | 9.328 mL | 18.656 mL |
5 mM | 37.311 µL | 186.557 µL | 373.113 µL | 1.866 mL | 3.731 mL |
10 mM | 18.656 µL | 93.278 µL | 186.557 µL | 932.784 µL | 1.866 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Rhodamine 800 *CAS 137993-41-0* | 682 | 704 | - | - |
Rhodamine B *CAS 81-88-9* | 546 | 567 | 1060001 | 0.71, 0.432 |
Rhodamine 6G *CAS 989-38-8* | 525 | 548 | 1160001 | 0.951 |
Rhodamine 110 *CAS 13558-31-1* | 500 | 522 | 80000 | - |
Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 | - |
Fluorescein aldehyde [5-FAM aldehyde] | 493 | 517 | 83000 | - |
Cy3 aldehyde | 555 | 569 | 1500001 | 0.151 |
Cy5 aldehyde | 651 | 670 | 2500001 | 0.271, 0.42 |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 |
Citations
View all 1 citations: Citation Explorer
Transient Competitors to Modulate Dynamic Covalent Cross-Linking of Recombinant Hydrogels
Authors: Gilchrist, Aidan E and Liu, Yueming and Klett, Katarina and Liu, Yu-Chung and Ceva, Sof{\'\i}a and Heilshorn, Sarah C
Journal: Chemistry of Materials (2023)
Authors: Gilchrist, Aidan E and Liu, Yueming and Klett, Katarina and Liu, Yu-Chung and Ceva, Sof{\'\i}a and Heilshorn, Sarah C
Journal: Chemistry of Materials (2023)
References
View all 50 references: Citation Explorer
Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982
Super-Resolution Fluorescence Imaging of Arabidopsis thaliana Transfer Cell Wall Ingrowths using Pseudo-Schiff Labelling Adapted for the Use of Different Dyes.
Authors: Rae, Angus E and Wei, Xiaoyang and Flores-Rodriguez, Neftali and McCurdy, David W and Collings, David A
Journal: Plant & cell physiology (2020): 1775-1787
Authors: Rae, Angus E and Wei, Xiaoyang and Flores-Rodriguez, Neftali and McCurdy, David W and Collings, David A
Journal: Plant & cell physiology (2020): 1775-1787
A novel rhodamine-based colorimetric and fluorometric probe for simultaneous detection of multi-metal ions.
Authors: Zhang, Yuqi and Wang, Wang and Li, Ran and Zhang, Ensheng and Li, Zhonghua and Tang, Long and Han, Bo and Hou, Xiufang and Wang, Ji-Jiang
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118050
Authors: Zhang, Yuqi and Wang, Wang and Li, Ran and Zhang, Ensheng and Li, Zhonghua and Tang, Long and Han, Bo and Hou, Xiufang and Wang, Ji-Jiang
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118050
A rhodamine-based turn-on nitric oxide sensor in aqueous medium with endogenous cell imaging: an unusual formation of nitrosohydroxylamine.
Authors: Alam, Rabiul and Islam, Abu Saleh Musha and Sasmal, Mihir and Katarkar, Atul and Ali, Mahammad
Journal: Organic & biomolecular chemistry (2018): 3910-3920
Authors: Alam, Rabiul and Islam, Abu Saleh Musha and Sasmal, Mihir and Katarkar, Atul and Ali, Mahammad
Journal: Organic & biomolecular chemistry (2018): 3910-3920
Fluorescent probes for detecting cysteine.
Authors: Yan, Fanyong and Sun, Xiaodong and Zu, Fanlin and Bai, Zhangjun and Jiang, Yingxia and Fan, Keqing and Wang, Jie
Journal: Methods and applications in fluorescence (2018): 042001
Authors: Yan, Fanyong and Sun, Xiaodong and Zu, Fanlin and Bai, Zhangjun and Jiang, Yingxia and Fan, Keqing and Wang, Jie
Journal: Methods and applications in fluorescence (2018): 042001
Inhibitory Effects of Benzaldehyde, Vanillin, Muscone and Borneol on P-Glycoprotein in Caco-2 Cells and Everted Gut Sac.
Authors: Wang, Shixiang and Tan, Nan and Ma, Cuicui and Wang, Jie and Jia, Pu and Liu, Jianghong and Yang, Yang and Xie, Zhanxiong and Zhao, Kun and Zheng, Xiaohui
Journal: Pharmacology (2018): 269-277
Authors: Wang, Shixiang and Tan, Nan and Ma, Cuicui and Wang, Jie and Jia, Pu and Liu, Jianghong and Yang, Yang and Xie, Zhanxiong and Zhao, Kun and Zheng, Xiaohui
Journal: Pharmacology (2018): 269-277
Reinvestigation of an O-Salicylaldehyde Ester Functional Group in Aqueous Buffer and Discovery of a Coumarin Scaffold Probe for Selective N-Terminal Cysteine Labeling.
Authors: Murale, Dhiraj P and Hong, Seong Cheol and Jang, Se-Young and Lee, Jun-Seok
Journal: Chembiochem : a European journal of chemical biology (2018): 2545-2549
Authors: Murale, Dhiraj P and Hong, Seong Cheol and Jang, Se-Young and Lee, Jun-Seok
Journal: Chembiochem : a European journal of chemical biology (2018): 2545-2549
The Protective Effect of Jatrorrhizine Against Oxidative Stress in Primary Rat Cortical Neurons.
Authors: Luo, Tao and Shen, Xiu-Yin and Li, Sheng and Ouyang, Ting and Mai, Quan-An and Wang, Hua-Qiao
Journal: CNS & neurological disorders drug targets (2017): 617-623
Authors: Luo, Tao and Shen, Xiu-Yin and Li, Sheng and Ouyang, Ting and Mai, Quan-An and Wang, Hua-Qiao
Journal: CNS & neurological disorders drug targets (2017): 617-623
Hyaluronate-Peanut Agglutinin Conjugates for Target-Specific Bioimaging of Colon Cancer.
Authors: Beack, Songeun and Cho, Minsoo and Kim, Young-Eun and Ahn, G-One and Hahn, Sei Kwang
Journal: Bioconjugate chemistry (2017): 1434-1442
Authors: Beack, Songeun and Cho, Minsoo and Kim, Young-Eun and Ahn, G-One and Hahn, Sei Kwang
Journal: Bioconjugate chemistry (2017): 1434-1442
Tunable uptake/release mechanism of protein microgel particles in biomimicking environment.
Authors: Pepe, A and Podesva, P and Simone, G
Journal: Scientific reports (2017): 6014
Authors: Pepe, A and Podesva, P and Simone, G
Journal: Scientific reports (2017): 6014
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
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What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?