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Rhodamine aldehyde [5-TAMRA aldehyde]

Chemical structure for Rhodamine aldehyde [5-TAMRA aldehyde].
Chemical structure for Rhodamine aldehyde [5-TAMRA aldehyde].
Ordering information
Price ()
Catalog Number9005
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight536.03
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (51)

OverviewpdfSDSpdfProtocol


Molecular weight
536.03
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.178
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)
552
Emission (nm)
578
Rhodamine aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated rhodamine dye can be easily detected by a common fluorescence instrument under TRITC or Cy3 channel.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine aldehyde [5-TAMRA aldehyde] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM186.557 µL932.784 µL1.866 mL9.328 mL18.656 mL
5 mM37.311 µL186.557 µL373.113 µL1.866 mL3.731 mL
10 mM18.656 µL93.278 µL186.557 µL932.784 µL1.866 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
Rhodamine 700 *CAS 63561-42-2*----
Rhodamine 800 *CAS 137993-41-0*682704--
Rhodamine B *CAS 81-88-9*54656810600010.311
Rhodamine 6G *CAS 989-38-8*52554811600010.951
Rhodamine 110 *CAS 13558-31-1*50052280000-
Rhodamine 123 *CAS 62669-70-9*50852885200-
Fluorescein aldehyde [5-FAM aldehyde]49351783000-
Cy3 aldehyde55556915000010.151
Cy5 aldehyde65167025000010.271, 0.42
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*499520730000.921
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References


View all 50 references: Citation Explorer
Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982
Super-Resolution Fluorescence Imaging of Arabidopsis thaliana Transfer Cell Wall Ingrowths using Pseudo-Schiff Labelling Adapted for the Use of Different Dyes.
Authors: Rae, Angus E and Wei, Xiaoyang and Flores-Rodriguez, Neftali and McCurdy, David W and Collings, David A
Journal: Plant & cell physiology (2020): 1775-1787
A novel rhodamine-based colorimetric and fluorometric probe for simultaneous detection of multi-metal ions.
Authors: Zhang, Yuqi and Wang, Wang and Li, Ran and Zhang, Ensheng and Li, Zhonghua and Tang, Long and Han, Bo and Hou, Xiufang and Wang, Ji-Jiang
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118050
A rhodamine-based turn-on nitric oxide sensor in aqueous medium with endogenous cell imaging: an unusual formation of nitrosohydroxylamine.
Authors: Alam, Rabiul and Islam, Abu Saleh Musha and Sasmal, Mihir and Katarkar, Atul and Ali, Mahammad
Journal: Organic & biomolecular chemistry (2018): 3910-3920
Fluorescent probes for detecting cysteine.
Authors: Yan, Fanyong and Sun, Xiaodong and Zu, Fanlin and Bai, Zhangjun and Jiang, Yingxia and Fan, Keqing and Wang, Jie
Journal: Methods and applications in fluorescence (2018): 042001
Inhibitory Effects of Benzaldehyde, Vanillin, Muscone and Borneol on P-Glycoprotein in Caco-2 Cells and Everted Gut Sac.
Authors: Wang, Shixiang and Tan, Nan and Ma, Cuicui and Wang, Jie and Jia, Pu and Liu, Jianghong and Yang, Yang and Xie, Zhanxiong and Zhao, Kun and Zheng, Xiaohui
Journal: Pharmacology (2018): 269-277
Reinvestigation of an O-Salicylaldehyde Ester Functional Group in Aqueous Buffer and Discovery of a Coumarin Scaffold Probe for Selective N-Terminal Cysteine Labeling.
Authors: Murale, Dhiraj P and Hong, Seong Cheol and Jang, Se-Young and Lee, Jun-Seok
Journal: Chembiochem : a European journal of chemical biology (2018): 2545-2549
The Protective Effect of Jatrorrhizine Against Oxidative Stress in Primary Rat Cortical Neurons.
Authors: Luo, Tao and Shen, Xiu-Yin and Li, Sheng and Ouyang, Ting and Mai, Quan-An and Wang, Hua-Qiao
Journal: CNS & neurological disorders drug targets (2017): 617-623
Hyaluronate-Peanut Agglutinin Conjugates for Target-Specific Bioimaging of Colon Cancer.
Authors: Beack, Songeun and Cho, Minsoo and Kim, Young-Eun and Ahn, G-One and Hahn, Sei Kwang
Journal: Bioconjugate chemistry (2017): 1434-1442
Tunable uptake/release mechanism of protein microgel particles in biomimicking environment.
Authors: Pepe, A and Podesva, P and Simone, G
Journal: Scientific reports (2017): 6014