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RPE-Cy5-streptavidin conjugate

PBMC cells were stained with (Red) or without (Green) mouse anti-CD45-Biotin and then followed with PE-Cy5-streptavidin conjugate stain. <br>Blue: Unstained cells.
PBMC cells were stained with (Red) or without (Green) mouse anti-CD45-Biotin and then followed with PE-Cy5-streptavidin conjugate stain. <br>Blue: Unstained cells.
Ordering information
Price ()
Catalog Number16918
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Spectral properties
Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Correction Factor (260 nm)
0.02
Correction Factor (280 nm)
0.03
Correction Factor (482 nm)
0.009
Correction Factor (565 nm)
0.09
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
651
Emission (nm)
670
Quantum yield
0.271, 0.42
Streptavidin conjugates are widely used together with a biotin conjugate for specific detection of a variety of biological molecules since streptavidin has a very high binding affinity for biotin and its conjugates. RPE-Cy5-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with RPE-Cy5 covalently attached (as the fluorescent label). It is used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy5® filter set. AAT Bioquest offers the largest selection of biotinylated and streptavidin conjugates and reagents. Some of them are not available from other commercial vendors.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42

References


View all 7 references: Citation Explorer
Tumor Extracellular pH-Driven Cancer-Selective Artificial Receptor-Mediated Tumor-Targeted Fluorescence Imaging.
Authors: Guo, Qian and Wu, Zhan and Peng, Yongbo and Peng, Wenyi and Huang, Qin and Peng, Miao and Huang, Ni and Hu, Xiaoxiao and Fu, Ting and Zhao, Zilong and Tan, Weihong
Journal: Analytical chemistry (2019): 13349-13354
Optimal Structure of a Plasmonic Chip for Sensitive Bio-Detection with the Grating-Coupled Surface Plasmon-Field Enhanced Fluorescence (GC-SPF).
Authors: Tawa, Keiko and Nakayama, Takuya and Kintaka, Kenji
Journal: Materials (Basel, Switzerland) (2017)
Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion.
Authors: Liu, Xiaoxia and Seven, Alpay Burak and Xu, Junjie and Esser, Victoria and Su, Lijing and Ma, Cong and Rizo, Josep
Journal: Nature protocols (2017): 2014-2028
Targeting prostate cancer cells with a multivalent PSMA inhibitor-guided streptavidin conjugate.
Authors: Liu, Tiancheng and Nedrow-Byers, Jessie R and Hopkins, Mark R and Wu, Lisa Y and Lee, Jeonghoon and Reilly, Peter T A and Berkman, Clifford E
Journal: Bioorganic & medicinal chemistry letters (2012): 3931-4
Use of a multiplexed CMOS microarray to optimize and compare oligonucleotide binding to DNA probes synthesized or immobilized on individual electrodes.
Authors: Maurer, Karl and Yazvenko, Nina and Wilmoth, Jodi and Cooper, John and Lyon, Wanda and Danley, David
Journal: Sensors (Basel, Switzerland) (2010): 7371-85
Spot arrays on modified glass surfaces for efficient SPOT synthesis and on-chip bioassay of peptides.
Authors: Kim, Do-Hyun and Shin, Dong-Sik and Lee, Yoon-Sik
Journal: Journal of peptide science : an official publication of the European Peptide Society (2007): 625-33
A simple and reliable method for the detection of sialylation in complex/hybrid-type carbohydrate chains of glycoproteins by mixed lectins.
Authors: Natsuki, Jun and Machida, Masayuki
Journal: Biotechnology and bioengineering (2006): 225-30