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RPE-Cy7-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This RPE-Cy7-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with RPE-Cy7 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by RPE–Cy7–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the RPE–Cy7-specific B13-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by RPE–Cy7–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the RPE–Cy7-specific B13-A channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
16916100 ug
Price
169171 mg
Price
 
Physical properties
Molecular weight~292000
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)778
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 13, 2025