Screen Quest™ Membrane Potential Assay Kit *Orange Fluorescence*

Screen Quest™ Membrane Potential Assay Kit

Orange Fluorescence

Screen Quest™ Membrane Potential Assay Kit is a homogeneous fluorescence-based kit for monitoring cellular membrane potential changes associated with ion channel activity.

Membrane potential detection: Uses a proprietary fluorescent indicator to monitor changes in cellular membrane potential
Orange fluorescence readout: Supports detection with recommended fluorescence reader settings of Ex/Em = 530/570 nm
Homogeneous microplate format: Supports 96-well and 384-well assays without washing after dye loading and can be adapted to automation
Research applications: Suitable for functional ion channel studies and screening compounds that alter cellular membrane potential

<p>ATP dose response in HEK cells transiently transfected with P2X receptor. HEK cells transiently transfected with P2X receptor were seeded overnight at 40,000 cells/100 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of the MP dye-loading solution in a 5% CO2, 37 °C incubator for 60 minutes. ATP (50 µL/well) was added by FlexStation to achieve the final indicated concentrations. The fluorescence signal was measured with bottom read mode at Ex/Em = 530/570 nm (cutoff at 550 nm).</p>

Protocol

Catalog	Size	Price
35999	1 plate	Price
36000	10 plates	Price
36001	100 plates	Price

Overview

Membrane potential is the difference in voltage between the interior and exterior of a cell. The membrane potential allows a cell to function as a battery, providing power to operate a variety of "molecular devices" embedded in the membrane. In electrically excitable cells such as neurons, membrane potential is used for transmitting signals between different parts of a cell. Opening or closing of ion channels at one point in the membrane produces a local change in the membrane potential, which causes electric current to flow rapidly to other points in the membrane. Ion channels have been identified as important drug discovery targets. Our Screen Quest™ Membrane Potential Assay Kit is a homogeneous assay with fast read time. It uses our proprietary long wavelength membrane potential indicator to detect the membrane potential change that is caused by the opening and closing of the ion channels. The red fluorescence of the membrane potential indicator used in the kit has enhanced fluorescence upon entering cells and minimizes the interferences resulted from the screening compounds and/or cellular autofluorescence.

Components

Catalog 35999 (1 plate)

Component A: MP Sensor	1 vial (15 µL)
Component B: 10X Assay Buffer	1 bottle (1 mL)
Component C: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (9 mL)

Catalog 36000 (10 plates)

Component A: MP Sensor	1 vial (150 µL)
Component B: 10X Assay buffer	1 bottle (10 mL)
Component C: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (100 mL)

Catalog 36001 (100 plates)

Component A: MP Sensor	10 vials (150 µL/vial)
Component B: 10X Assay buffer	1 bottle (100 mL)
Component C: HHBS (Hanks' buffer with 20 mM Hepes)	Not included

References

View all 52 references: Citation Explorer

A rapid flow cytometry assay for the assessment of calcium mobilization in human neutrophils in a small volume of lysed whole-blood

Authors:

Desmeules P, Dufour M, Fern and es MJ., undefined

Journal:

J Immunol Methods (2009): 154

A novel high-throughput screening assay for HCN channel blocker using membrane potential-sensitive dye and FLIPR

Authors:

Vasilyev DV, Shan QJ, Lee YT, Soloveva V, Nawoschik SP, Kaftan EJ, Dunlop J, Mayer SC, Bowlby MR.

Journal:

J Biomol Screen (2009): 1119

A high-capacity membrane potential FRET-based assay for the sodium-coupled glucose co-transporter SGLT1

Authors:

Weinglass AB, Swensen AM, Liu J, Schmalhofer W, Thomas A, Williams B, Ross L, Hashizume K, Kohler M, Kaczorowski GJ, Garcia ML.

Journal:

Assay Drug Dev Technol (2008): 255

Development of an aequorin luminescence calcium assay for high-throughput screening using a plate reader, the LumiLux

Authors:

Menon V, Ranganathn A, Jorgensen VH, Sabio M, Christoffersen CT, Uberti MA, Jones KA, Babu PS.

Journal:

Assay Drug Dev Technol (2008): 787

A high-throughput assay for evaluating state dependence and subtype selectivity of Cav2 calcium channel inhibitors

Authors:

Dai G, Haedo RJ, Warren VA, Ratliff KS, Bugianesi RM, Rush A, Williams ME, Herrington J, Smith MM, McManus OB, Swensen AM.

Journal:

Assay Drug Dev Technol (2008): 195

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	530 nm
Emission	570 nm
Cutoff	550 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Other instruments	FDSS, FLIPR, FlexStation, NOVOStar

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 6, 2026