Screen Quest™ TR-FRET No Wash cAMP Assay Kit

Screen Quest™ TR-FRET No Wash cAMP Assay Kit is a homogeneous competitive immunoassay for measuring cAMP using time-resolved fluorescence resonance energy transfer in microplate assays.

Competitive cAMP detection: Sample cAMP competes with the trFluor™ 650-labeled cAMP tracer for binding to the trFluor™ Eu-labeled anti-cAMP antibody
Time-resolved FRET readout: Antibody-bound tracer generates a TR-FRET signal that decreases as sample cAMP increases
No-wash assay format: Requires neither washing nor sample acetylation and supports 96-well and 384-well microplates
Research applications: Suitable for measuring cellular cAMP responses and screening compounds that modulate GPCR-mediated cAMP signaling

cAMP does response was measured with Screen Quest™ TR-FRET No Wash cAMP Assay Kit (Cat #36379) and fluorescence was measured on CLARIOstar microplate reader (BMG Labtech). The assay demonstrated a detection sensitivity as low as 1 nM cAMP.

Protocol

Catalog	Size	Price
36379	1 plate	Price
36380	10 plates	Price
36381	50 plates	Price

Overview

Screen Quest™ TR-FRET No Wash cAMP Assay Kit provides a convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. Compared to other commercial ELISA cAMP assay kits, this homogenous cAMP assay kit does not require a wash step or the acetylation step. The assay is based on the competition for a fixed number of anti-cAMP antibody binding sites between the trFluor™ 650 labeled cAMP tracer and non-labeled free cAMP. The anti-cAMP antibody is labeled with trFluor™ Eu while the cAMP tracer is labeled with trFluor™ 650. In the absence of cAMP, trFluor™ 650-cAMP conjugate is bound to trFluor™ Eu-labeled anti-cAMP antibody exclusively to have a strong FRET signal. While the unlabeled free cAMP is present in the test sample, it competes for the trFluor™ Eu-labeled anti-cAMP antibody conjugate binding sites, therefore inhibits the binding of trFluor™ 650-cAMP to anti-cAMP antibody. The trFluor™ 650 labeled cAMP tracer only has fluorescence lifetime of nanosecond while TR Fluor™ Eu-labeled anti-cAMP antibody-bound fluorescent cAMP tracer has much longer fluorescence lifetime of lanthanide fluorophore. The magnitude of time -resolved fluorescence signal (TR- FRET) signal is proportional to the concentration of cAMP in a sample. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format, and is convenient for monitoring the cAMP activity with ultra-specificity and sensitivity in G-protein coupled receptor systems.

Components

Catalog 36379 (1 plate)

Component A: Anti cAMP-trFluor™ Eu	1 vial
Component B: cAMP-trFluor™ 650	1 vial
Component C: cAMP Standard	1 vial (33 μg)
Component D: Cell Lysis Buffer	1 bottle (10 mL)
Component E: Diluent	1 bottle (10 mL)

Catalog 36380 (10 plates)

Component A: Anti cAMP-trFluor™ Eu	1 vial
Component B: cAMP-trFluor™ 650	1 vial
Component C: cAMP Standard	1 vial (33 μg)
Component D: Cell Lysis Buffer	1 bottle (100 mL)
Component E: Diluent	1 bottle (100 mL)

Catalog 36381 (50 plates)

Component A: Anti cAMP-trFluor™ Eu	5 vials
Component B: cAMP-trFluor™ 650	5 vials
Component C: cAMP Standard	1 vial (33 μg)
Component D: Cell Lysis Buffer	5 bottles (100 mL/bottle)
Component E: Diluent	5 bottles (100 mL/bottle)

Citations

View all 2 citations: Citation Explorer

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling

Authors:

Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others, undefined

Journal:

Scientific Reports (2016)

The M2 muscarinic receptors are essential for signaling in the heart left ventricle during restraint stress in mice

Authors:

Tomankova, Hana and Valuskova, Paulina and Varejkova, Eva and Rotkova, Jana and Benes, Jan and Myslivecek, Jaromir

Journal:

Stress (2015)

References

View all 132 references: Citation Explorer

cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation

Authors:

Rodriguez P, Rojas J.

Journal:

J Cell Biochem (2016): 741

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism

Authors:

Alqurashi M, Gehring C, Marondedze C.

Journal:

Int J Mol Sci (2016): 852

Role of the cAMP Pathway in Glucose and Lipid Metabolism

Authors:

Ravnskjaer K, Madiraju A, Montminy M.

Journal:

Handb Exp Pharmacol (2016): 29

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Authors:

Miazzi F, Hansson BS, Wicher D.

Journal:

J Exp Biol (2016): 1798

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death

Authors:

Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.

Journal:

Cell Death Dis (2016): e2198

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Recommended plate	Solid black and/or Black wall/clear bottom
Instrument specification(s)	Time-resolved

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Page updated on October 13, 2026