Name: StrandBrite™ Green Fluorimetric RNA Quantitation Kit
Brand: AAT Bioquest
SKU: 17656
Price: 348 USD
Availability: InStock

StrandBrite™ Green Fluorimetric RNA Quantitation Kit

Detecting and quantitating small amounts of RNA is extremely important for a wide variety of molecular biology procedures such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR. The most commonly used technique for measuring nucleic acid concentration is the determination of absorbance at 260 nm. The major disadvantage of the absorbance-based method is the interferences caused by proteins, free nucleotides and other UV absorbing compounds. The use of sensitive, fluorescent nucleic acid stains alleviates this interference problem. StrandBrite™ RNA quantifying reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. StrandBrite™ RNA quantifying reagent can detect as little as 5 ng/mL RNA with a fluorescence microplate reader or fluorometer. Our StrandBrite™ Green Fluorimetric RNA Quantitation Kit includes our StrandBrite™ Green nucleic acid stain with an optimized and robust protocol. It provides a convenient method for quantifying RNA in solutions.

RNA dose response with StrandBrite™ Green in cuvettes and measured using a Cary eclipse spectrofluorometer.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17656	100 Tests	Price

References

View all 31 references: Citation Explorer

Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay

Authors:

Peterson EJ, Kireev D, Moon AF, Midon M, Janzen WP, Pingoud A, Pedersen LC, Singleton SF.

Journal:

J Biomol Screen (2013): 247

Validation of a PicoGreen-based DNA quantification integrated in an RNA extraction method for two-dimensional and three-dimensional cell cultures

Authors:

Chen Y, Sonnaert M, Roberts SJ, Luyten FP, Schrooten J.

Journal:

Tissue Eng Part C Methods (2012): 444

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen dye

Authors:

Moreno LA, Cox KL.

Journal:

J Vis Exp. (2010)

Metal-enhanced PicoGreen fluorescence: application to fast and ultra-sensitive pg/ml DNA quantitation

Authors:

Dragan AI, Bishop ES, Casas-Finet JR, Strouse RJ, Schenerman MA, Geddes CD.

Journal:

J Immunol Methods (2010): 95

Comparison of SYBR Green I-, PicoGreen-, and [3H]-hypoxanthine-based assays for in vitro antimalarial screening of plants from Nigerian ethnomedicine

Authors:

Abiodun OO, Gbotosho GO, Ajaiyeoba EO, Happi CT, Hofer S, Wittlin S, Sowunmi A, Brun R, Oduola AM.

Journal:

Parasitol Res (2010): 933

Spectral properties

Excitation (nm)	509
Emission (nm)	527

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
UNSPSC	41116134

Instrument settings

Spectrofluorometer
Excitation	490 nm
Emission	545 nm
Cutoff	515 nm

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Page updated on October 9, 2025

Component A: StrandBrite™ Green	1 vial (0.5 mL, 200X in DMSO)
Component B: 10X Assay Buffer	1 bottle (20 mL)
Component C: RNA Standard	1 vial (0.2 mL, 100 µg/mL)