logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
StrandBrite™ Green RNA Quantifying Reagent
200X DMSO Solution
Detecting and quantitating small amounts of RNA is extremely important for a wide variety of molecular biology procedures such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR. The most commonly used technique for measuring nucleic acid concentration is the determination of absorbance at 260 nm. The major disadvantage of the absorbance-based method is the interferences caused by proteins, free nucleotides and other UV absorbing compounds. The use of sensitive, fluorescent nucleic acid stains alleviates this interference problem. StrandBrite™ RNA quantifying reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. It is a positively charged fluorescent probe that binds to the hydrophobic pockets of RNA and forms a highly luminescent complex through the synergistic actions of stacking, hydrophobic forces, hydrogen bonding and electrostatic interactions. StrandBrite™ RNA quantifying reagent has extremely low inherent fluorescence that is significantly enhanced upon binding to RNAs, resulting in a great enhancement in its fluorescence. StrandBrite™ RNA quantifying reagent can detect as little as 5 ng/mL RNA with a fluorescence microplate reader or fluorometer.
RNA dose response with StrandBrite™ Green in a solid black 96-well microplate and measured using a Gemini microplate reader (Molecular Devices).
RNA dose response with StrandBrite™ Green in a solid black 96-well microplate and measured using a Gemini microplate reader (Molecular Devices).
protocol
Protocol
COA
CatalogSize
Price
Quantity
176101 ml
Price
1761110 ml
Price
 
Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)509
Emission (nm)527
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 13, 2025