TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
Related products
Overview | SDSProtocol |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. The master mix delivers results within 50 minutes for 40 cycles of PCR in a 20 uL reaction volume. The mix includes an optimized buffer containing dNTPs and our proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase enzyme, an enzyme designed to allow instant hot start which minimizes non-specific product formation thus allowing room temperature reaction setup. Only template and target primers are required to run the desired PCR reactions. TAQuest™ FAST qPCR Master Mix with Helixyte™ Green ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. The Helixyte Green intercalating dye allows rapid DNA detection and analysis without using sequence-specific probes. This master mix does not contain a ROX reference dye.
Platform
qPCR
Instrument specification(s) | SYBR Green filter |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used as a guideline.
Note Thaw the TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* at room temperature. Vortex qPCR Master Mix thoroughly before use.
Table 2.Thermal cycling parameters
Note Thaw the TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* at room temperature. Vortex qPCR Master Mix thoroughly before use.
- Prepare one of the following reaction mixes as indicated in Table 1.
- Carefully mix the reagents with a gentle vortex followed by a brief centrifuge.
- Set up the plate in the qPCR instrument and run as indicated in Table 2.
Components | Volume (25 µL/reaction) | Volume (50 µL/reaction) | Final Conc. |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* | 12.5 µL | 25 µL | 1X |
Upstream primer, 10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
Downstream primer, 10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
DNA template | 1-5 µL | 1-5 µL | Optimized conc. |
Nuclease-Free Water to | 25 µL | 50 µL |
Parameter | Polymerase Activation | PCR (30-40 cycles) | |
Hold | Denature | Anneal/Extend | |
Temperature | 95 °C | 95 °C | 60 °C |
Time (m:ss) | 0:10 | 0:20 | 0:30 |
Images
References
View all 10 references: Citation Explorer
Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D'Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D'Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423
Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8
Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)
A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17
A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7
Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA.
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53
Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles.
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6
Detection of equine herpesvirus-1 in nasal swabs of horses by quantitative real-time PCR.
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine : 1234-8
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine : 1234-8
Application notes
Standard vs. Touchdown PCR
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
FAQ
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How can I lyse my cells without lysing the nuclear membrane?
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Do you have any fixable mitochondria staining assay kits?