Tide Quencher™ 3WS (TQ3WS) is an advanced non-fluorescent quencher engineered to outperform conventional quenchers for TAMRA, TF3, and Cy3 dyes. TQ3WS offers several key advantages:

1. Significantly higher molar absorption coefficients
2. Enhanced quenching efficiencies
3. Excellent aqueous solubility
4. A variety of functional derivatives suitable for conjugation to oligonucleotides and peptides

Notably, TQ3WS is the most water-soluble non-fluorescent quencher currently available for oligonucleotide and peptide applications. Experimental data confirm that conjugation with TQ3WS significantly enhances the aqueous solubility of hydrophobic peptide sequences without compromising functional integrity. Additionally, the maleimide-functionalized derivative of TQ3WS exhibits high selectivity and reactivity toward thiol groups under mildly acidic to neutral pH (6.5–7.5), enabling efficient and stable thioether bond formation. These properties make TQ3WS an ideal quencher for demanding bioconjugation and assay development workflows.