Tide Quencher™ 7.1WS azide [TQ7.1WS azide]
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1046.12 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 7.1WS amine [TQ7.1WS amine] |
Tide Quencher™ 7.1WS maleimide [TQ7.1WS maleimide] |
Tide Quencher™ 7.1WS acid [TQ7.1WS acid] |
Tide Quencher™ 7.1WS alkyne [TQ7.1WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1046.12 |
Tide Quencher™ 7.1WS (TQ7.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.1WS Azide is an excellent building block for preparing TQ5.1WS-labeled probes from alkyne-modified oligonucleotides (including DBCO-modified oligonucleotides) and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7.1WS azide [TQ7.1WS azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 95.591 µL | 477.957 µL | 955.913 µL | 4.78 mL | 9.559 mL |
5 mM | 19.118 µL | 95.591 µL | 191.183 µL | 955.913 µL | 1.912 mL |
10 mM | 9.559 µL | 47.796 µL | 95.591 µL | 477.957 µL | 955.913 µL |
Molarity calculator
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References
View all 13 references: Citation Explorer
Quantitative and biosafe modification of bifunctional groups onto carbon dots by click chemistry.
Authors: He, Qian and Wu, Zewen and Li, Jingxuan and Li, Ruijiao and Zhang, Liyun and Liu, Yaodong
Journal: Journal of materials chemistry. B (2023): 5094-5100
Authors: He, Qian and Wu, Zewen and Li, Jingxuan and Li, Ruijiao and Zhang, Liyun and Liu, Yaodong
Journal: Journal of materials chemistry. B (2023): 5094-5100
Loading Intracranial Drug-Eluting Reservoirs Across the Blood-Brain Barrier With Focused Ultrasound.
Authors: Moody, Christopher T and Durham, Phillip G and Dayton, Paul A and Brudno, Yevgeny
Journal: Ultrasound in medicine & biology (2023): 1679-1685
Authors: Moody, Christopher T and Durham, Phillip G and Dayton, Paul A and Brudno, Yevgeny
Journal: Ultrasound in medicine & biology (2023): 1679-1685
Differential Translation Activity Analysis Using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) in Archaea.
Authors: Kern, Michael and Ferreira-Cerca, Sébastien
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 229-246
Authors: Kern, Michael and Ferreira-Cerca, Sébastien
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 229-246
Controlling Coagulation in Blood with Red Light.
Authors: Müller, Patricia and Sahlbach, Marlen and Gasper, Simone and Mayer, Günter and Müller, Jens and Pötzsch, Bernd and Heckel, Alexander
Journal: Angewandte Chemie (International ed. in English) (2021): 22441-22446
Authors: Müller, Patricia and Sahlbach, Marlen and Gasper, Simone and Mayer, Günter and Müller, Jens and Pötzsch, Bernd and Heckel, Alexander
Journal: Angewandte Chemie (International ed. in English) (2021): 22441-22446
Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Bioorthogonally Applicable Fluorescence Deactivation Strategy for Receptor Kinetics Study and Theranostic Pretargeting Approaches.
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)
Erlotinib-Guided Self-Assembled Trifunctional Click Nanotheranostics for Distinguishing Druggable Mutations and Synergistic Therapy of Nonsmall Cell Lung Cancer.
Authors: Gao, Yu and Zhang, Huijuan and Zhang, Yingying and Lv, Tingting and Zhang, Lu and Li, Ziying and Xie, Xiaodong and Li, Fengqiao and Chen, Haijun and Jia, Lee
Journal: Molecular pharmaceutics (2018): 5146-5161
Authors: Gao, Yu and Zhang, Huijuan and Zhang, Yingying and Lv, Tingting and Zhang, Lu and Li, Ziying and Xie, Xiaodong and Li, Fengqiao and Chen, Haijun and Jia, Lee
Journal: Molecular pharmaceutics (2018): 5146-5161
Folate and Heptamethine Cyanine Modified Chitosan-Based Nanotheranostics for Tumor Targeted Near-Infrared Fluorescence Imaging and Photodynamic Therapy.
Authors: Zhang, Yingying and Lv, Tingting and Zhang, Huijuan and Xie, Xiaodong and Li, Ziying and Chen, Haijun and Gao, Yu
Journal: Biomacromolecules (2017): 2146-2160
Authors: Zhang, Yingying and Lv, Tingting and Zhang, Huijuan and Xie, Xiaodong and Li, Ziying and Chen, Haijun and Gao, Yu
Journal: Biomacromolecules (2017): 2146-2160
NIR-Cyanine Dye Linker: a Promising Candidate for Isochronic Fluorescence Imaging in Molecular Cancer Diagnostics and Therapy Monitoring.
Authors: Komljenovic, Dorde and Wiessler, Manfred and Waldeck, Waldemar and Ehemann, Volker and Pipkorn, Ruediger and Schrenk, Hans-Hermann and Debus, Jürgen and Braun, Klaus
Journal: Theranostics (2016): 131-41
Authors: Komljenovic, Dorde and Wiessler, Manfred and Waldeck, Waldemar and Ehemann, Volker and Pipkorn, Ruediger and Schrenk, Hans-Hermann and Debus, Jürgen and Braun, Klaus
Journal: Theranostics (2016): 131-41
Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation.
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65