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Tide Quencher™ 7.1WS azide [TQ7.1WS azide]

Tide Quencher™ 7.1WS (TQ7.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.1WS Azide is an excellent building block for preparing TQ5.1WS-labeled probes from alkyne-modified oligonucleotides (including DBCO-modified oligonucleotides) and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.

References

View all 13 references: Citation Explorer
Loading Intracranial Drug-Eluting Reservoirs Across the Blood-Brain Barrier With Focused Ultrasound.
Authors: Moody, Christopher T and Durham, Phillip G and Dayton, Paul A and Brudno, Yevgeny
Journal: Ultrasound in medicine & biology (2023): 1679-1685
Quantitative and biosafe modification of bifunctional groups onto carbon dots by click chemistry.
Authors: He, Qian and Wu, Zewen and Li, Jingxuan and Li, Ruijiao and Zhang, Liyun and Liu, Yaodong
Journal: Journal of materials chemistry. B (2023): 5094-5100
Differential Translation Activity Analysis Using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) in Archaea.
Authors: Kern, Michael and Ferreira-Cerca, Sébastien
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 229-246
Controlling Coagulation in Blood with Red Light.
Authors: Müller, Patricia and Sahlbach, Marlen and Gasper, Simone and Mayer, Günter and Müller, Jens and Pötzsch, Bernd and Heckel, Alexander
Journal: Angewandte Chemie (International ed. in English) (2021): 22441-22446
Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Page updated on December 6, 2024

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Catalog Number2120
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Physical properties

Molecular weight

1046.12

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure