XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 432.45 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.25 |
Correction Factor (280 nm) | 0.19 |
Extinction coefficient (cm -1 M -1) | 19000 |
Excitation (nm) | 343 |
Emission (nm) | 441 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
Molecular weight 432.45 | Correction Factor (260 nm) 0.25 | Correction Factor (280 nm) 0.19 | Extinction coefficient (cm -1 M -1) 19000 | Excitation (nm) 343 | Emission (nm) 441 |
XFD350 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 350 (Alexa Fluor® is the trademark of ThermoFisher). For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. XFD350 tyramide contains the bright XFD350 dye that can be readily detected with the standard FITC filter set.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 231.241 µL | 1.156 mL | 2.312 mL | 11.562 mL | 23.124 mL |
5 mM | 46.248 µL | 231.241 µL | 462.481 µL | 2.312 mL | 4.625 mL |
10 mM | 23.124 µL | 115.62 µL | 231.241 µL | 1.156 mL | 2.312 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.25 |
Correction Factor (280 nm) | 0.19 |
Extinction coefficient (cm -1 M -1) | 19000 |
Excitation (nm) | 343 |
Emission (nm) | 441 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide* | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide* | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide* | 590 | 618 | 92000 | 0.661 | 0.43 | 0.56 |
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide* | 579 | 603 | 88000 | 0.691 | 0.45 | 0.46 |
Citations
View all 2 citations: Citation Explorer
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
Authors: J, undefined and ura, A., Hu, J., Wilk, R., Krause, H. M.
Journal: J Vis Exp (2017): se name="11070.enl" path="C:\Website\Referenc
Authors: J, undefined and ura, A., Hu, J., Wilk, R., Krause, H. M.
Journal: J Vis Exp (2017): se name="11070.enl" path="C:\Website\Referenc
Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide
Authors: Schmidt, B. F., Chao, J., Zhu, Z., DeBiasio, R. L., Fisher, G.
Journal: J Histochem Cytochem (1997): 365-73
Authors: Schmidt, B. F., Chao, J., Zhu, Z., DeBiasio, R. L., Fisher, G.
Journal: J Histochem Cytochem (1997): 365-73