Application Notes

Introduction 6-ROX and its derivative are widely used for labeling oligonucleotides and automated DNA sequencing applications. Although ROX exhibit excellent spectral properties for labeling biological molecules, ROX acid and SE compounds are quite unstable. They degrade quite significantly…

Categories: Oligonucleotide Labeling
Posted January 11, 2022

Below is a list of common abbreviations found in peptide and oligonucleotide synthesis. Use CTRL + F to search this list Abbreviation Stands For Also Known As Examples β-Ala beta-alanine β-Alanine   1-Nal β-(1-Naphthyl)-L-alanine 3-(1-Naphthyl)-L-alanine   2-Nal β-(2-Naphthyl)-L-alanine  …

Categories: Oligonucleotide Labeling, Peptide Labeling
Posted April 24, 2020

Introduction Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency for longer wavelength dyes (such as fluoresceins, rhodamines, and cyanines) has limited its use in the development of sensitive FRET probes. AAT Bioquest has developed the robust Tide…

Categories: Fluorescence Resonance Energy Transfer (FRET), Oligonucleotide Labeling, Peptide Labeling
Posted January 3, 2020

Tide Fluor™ Dyes for Fluorescence Labeling and FRET Applications Optimized to label peptides and nucleotides with performance comparable to Alexa Fluor® Dyes Optimized to pair with Tide Quencher™ dark acceptors to maximize FRET efficiency pH-insensitive and environment-insensitive fluorescence…

Categories: Fluorescence Resonance Energy Transfer (FRET), Oligonucleotide Labeling, Peptide Labeling
Posted January 3, 2020

Introduction Proteases play essential roles in protein activation, cell regulation and signaling, as well as in the generation of amino acids for protein synthesis or utilization in other metabolic pathways. Although EDANS/DABCYL and MCA/DNP are widely used to develop a variety of FRET protease…

Categories: Fluorescence Resonance Energy Transfer (FRET), Oligonucleotide Labeling, Peptide Labeling
Posted January 2, 2020

Introduction Fluorescence resonance energy transfer (FRET) is the transfer of the excited state energy from the initially excited donor (D) to an acceptor (A). The donor molecules typically emit at shorter wavelengths that overlap with the absorption of acceptors. The process is a distance-dependent…

Categories: Fluorescence Resonance Energy Transfer (FRET), Oligonucleotide Labeling, Peptide Labeling
Posted January 2, 2020

Introduction Fluorescent peptides are essential tools used extensively in drug discovery and disease diagnosis. Although EDANS, FAM, and TAMRA are widely used to label peptides, their short absorption wavelengths, low extinction coefficients, and pH-sensitive fluorescence have limited their…

Categories: Fluorescence Resonance Energy Transfer (FRET), Oligonucleotide Labeling, Peptide Labeling
Posted December 17, 2019

Introduction Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency of longer wavelength dyes (such as fluoresceins, rhodamines and cyanines) has limited its use in the development of sensitive fluorogenic FRET probes. Additionally, the absorption…

Categories: Oligonucleotide Labeling, Peptide Labeling
Posted September 10, 2019

Labeling of Amino-Modified Oligonucleotides with Dye NHS esters Reaction Setup: Prepare a stock solution of 1 mg of the NHS-activated dye in 50 µL of anhydrous dimethyl formamide (DMF) or dimethyl sulfoxide (DMSO). 20 µL of this dye stock solution are added to a solution containing 20 - 30 nmol of the…

Categories: Oligonucleotide Labeling
Posted June 5, 2019

Introduction As the use of synthetic oligonucleotides in biomedical research gets more sophisticated, the simple modification of oligonucleotides becomes an urgent need. Although the automated oligonucleotide synthesis rapidly advances, there are still challenges in meeting the needs for…

Categories: Oligonucleotide Labeling
Posted December 20, 2013