Tide Quencher™ Dyes for FRET Applications

Introduction

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Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency for longer wavelength dyes (such as fluoresceins, rhodamines, and cyanines) has limited its use in the development of sensitive FRET probes. AAT Bioquest has developed the robust Tide Quencher™ acceptor dyes for development of longer wavelength FRET probes. These Tide Quencher™ dark FRET acceptors are optimized to pair with ourbright Tide Fluor™ dyes and the classic fluorophores (such as EDANS, FAM, TAMRA, HEX, JOE, TET, ROX, Cy3, Cy5, and CY7). Like our Tide Fluor™ donor dyes, our Tide Quencher™ acceptor dyes are much more cost-effective with comparable or even better performance for your desired biological applications than the other commercial products on the market.

1. BHQ is the is the trademark of Biosearch Technologies, Inc. Texas Red^®, QSY^® and Alexa Fluor^® are the trademarks of Molecular Probes. CyDye, Cy3^®, Cy5^®, Cy5.5^® and Cy7^® are the trademarks of GE Health Care. DyLight™ is the trademark of ThermoFisher Corp. IRDye^® 700 and IRDye^® 800 are the trademarks of Li-COR. Tide Quencher™ and Tide Quencher™ are the trademarks of AAT Bioquest.

Chemical Properties of Tide Quencher™ Non-Fluorescent Labeling Dyes

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AAT Bioquest offers a full set of Tide Quencher™ dyes that span the full visible spectrum. Tide Quencher™ dyes have improved labeling performance than the classic quencher dyes such as Dabcyl, Black Hole Quencher^® and QSY^® dyes. They can be readily used to develop various FRET probes for molecular diagnostics, proteomics and other biological applications.

1. * The molecular weight of the desired conjugate = the molecular weight of the free unlabeled molecule + the value listed in the table.

Spectral Properties of Tide Quencher™ Non-Fluorescent Labeling Dyes

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Tide Quencher™ dyes are a series of excellent labeling quenchers that span the full visible spectrum. Tide Quencher™ dyes have improved labeling performance than the classic quencher dyes such as Dabcyl, Black Hole Quencher^® and QSY^® dyes. They are the best affordable dyes for labeling oligos and peptides without comprised performance. Each Tide Quencher™ dye is developed to match the spectral properties of a particular fluorescent donor, e.g., Alexa Fluor^® or other labeling dyes (such as DyLight™ dyes).

1. Extinction Coefficient at their maximum absorption wavelength
2. CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligo and nucleic acid labeling)
3. CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptide and protein labeling).