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AAT Bioquest

Tide Quencher™ Dyes for FRET Applications

Introduction


Dye maleimides
Dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency for longer wavelength dyes (such as fluoresceins, rhodamines, and cyanines) has limited its use in the development of sensitive FRET probes. AAT Bioquest has developed the robust Tide Quencher™ acceptor dyes for development of longer wavelength FRET probes. These Tide Quencher™ dark FRET acceptors are optimized to pair with ourbright Tide Fluor™ dyes and the classic fluorophores (such as EDANS, FAM, TAMRA, HEX, JOE, TET, ROX, Cy3, Cy5, and CY7). Like our Tide Fluor™ donor dyes, our Tide Quencher™ acceptor dyes are much more cost-effective with comparable or even better performance for your desired biological applications than the other commercial products on the market.

 

Table 1. Tide Quencher™ Dyes for Developing FRET Probes

Dark FRET Acceptor
λmax (nm)
Features and Benefits
Ordering Information
Tide Quencher™ 1 (TQ1)490
  • Alternative to Dabcyl, QSY® 35 and BHQ®-0
  • Best paired with Tide Fluor™ 1 (TF1)
  • Excellent FRET efficiency with coumarins
Cat# 2188 (TQ1 azide, click chemistry)
Cat# 2189 (TQ1 alkyne, click chemistry)
Cat# 2190 (TQ1 acid)
Cat# 2192 (TQ1 amine)
Cat# 2193 (TQ1 CPG, OH-reactive)
Cat# 2196 (TQ1 maleimide, SH-reactive)
Cat# 2198 (TQ1 phosphoramidite, OH-reactive)
Cat# 2199 (TQ1 SE, NH2-reactive)
Tide Quencher™ 2 (TQ2)515
  • Alternative to BHQ®-1
  • Best paired with Tide Fluor™ 2 (TF2)
  • Better matched with FAM, FITC and Alexa Fluor® 488 than other commercial quenchers
Cat# 2211 (TQ2 azide, click chemistry)
Cat# 2212 (TQ2 alkyne, click chemistry)
Cat# 2200 (TQ2 acid)
Cat# 2202 (TQ2 amine)
Cat# 2203 & 2204 (TQ2 CPG, OH-reactive)
Cat# 2206 (TQ2 maleimide, SH-reactive)
Cat# 2208 (TQ2 phosphoramidite, OH-reactive)
Cat# 2210 (TQ2 SE, NH2-reactive)
Tide Quencher™ 2WS (TQ2WS)515
  • Alternative to BHQ®-1
  • Best paired with Tide Fluor™ 2 (TF2)
  • Better matched with FAM, FITC and Alexa Fluor® 488 than other commercial quenchers
Cat# 2050 (TQ2WS acid)
Cat# 2058 (TQ2WS SE, NH2-reactive)
Tide Quencher™ 3 (TQ3)570
  • Alternative to QSY® 7, QSY® 9 and BHQ®-2
  • Best paired with Tide Fluor™ 3 (TF3)
  • Excellent FRET efficiency with Cy3®, Alexa Fluor® 555 and TAMRA than other commercial quenchers
Cat# 2200 (TQ3 acid)
Cat# 2222 (TQ3 amine)
Cat# 2223 & 2224 (TQ3 CPG, OH-reactive)
Cat# 2226 (TQ3 maleimide, SH-reactive)
Cat# 2228 (TQ3 phosphoramidite, OH-reactive)
Cat# 2230 (TQ3 SE, NH2-reactive)
Cat# 2231 (TQ3 azide, click chemistry)
Cat# 2232 (TQ3 alkyne, click chemistry)
Tide Quencher™ 3WS (TQ3WS)578
  • Alternative to QSY® 7, QSY® 9 and BHQ®-2
  • Best paired with Tide Fluor™ 3 (TF3)
  • Excellent FRET efficiency with Cy3®, Alexa Fluor® 555 and TAMRA than other commercial quenchers
Cat# 2227 (TQ3WS acid)
Cat# 2229 (TQ3WS SE, NH2-reactive)
Tide Quencher™ 4 (TQ4)603
  • Strong absorption
  • Best paired with Tide Fluor™ 4 (TF4)
  • Better FRET efficiency with ROX, Texas Red® and Alexa Fluor® 594 than other commercial quenchers
Cat# 2062 & 2063 (TQ4 CPG, OH-reactive)
Tide Quencher™ 4WS (TQ4WS)~590
  • Strong absorption
  • Best paired with Tide Fluor™ 4 (TF4)
  • Better FRET efficiency with ROX, Texas Red® and Alexa Fluor® 594 than other commercial quenchers
Cat# 2060 (TQ4WS acid)
Cat# 2061 (TQ4WS amine)
Cat# 2064 (TQ4WS maleimide, SH-reactive)
Cat# 2067 (TQ4WS SE, NH2-reactive)
Cat# 2068 (TQ4WS azide, click chemistry)
Cat# 2069 (TQ4WS alkyne, click chemistry)
Tide Quencher™ 5 (TQ5)~670
  • Alternative to QSY® 21 and BHQ®-3
  • Best paired with Tide Fluor™ 5 (TF5)
  • Excellent FRET efficiency with Cy5®, DyLight® 649 and Alexa Fluor®647
Cat# 2077 & 2078 (TQ5 CPG, OH-reactive)
Tide Quencher™ 5WS (TQ5WS)~670
  • Alternative to QSY® 21 and BHQ®-3
  • Best paired with Tide Fluor™ 5 (TF5)
  • Excellent FRET efficiency with Cy5®, DyLight® 649 and Alexa Fluor®647
Cat# 2075 (TQ5WS acid)
Cat# 2076 (TQ5WS amine)
Cat# 2079 (TQ5WS maleimide, SH-reactive)
Cat# 2081 (TQ5WS SE, NH2-reactive)
Cat# 2082 (TQ5WS azide, click chemistry)
Cat# 2083 (TQ5WS alkyne, click chemistry)
Tide Quencher™ 6WS (TQ6WS)~700
  • Stronger absorption
  • Best paired with Tide Fluor™ 6 (TF6)
  • Better FRET efficiency with Cy5.5®, IRDye® 700 and Alexa Fluor® 680 than other commercial quenchers
Cat# 2090 (TQ6WS acid)
Cat# 2091 (TQ6WS amine)
Cat# 2094 (TQ6WS maleimide, SH-reactive)
Cat# 2096 (TQ6WS SE, NH2-reactive)
Cat# 2097 (TQ6WS azide, click chemistry)
Cat# 2098 (TQ5WS alkyne, click chemistry)
Tide Quencher™ 7WS (TQ7WS)~760
  • Stronger absorption
  • Best paired with Tide Fluor™ 7 (TF7)
  • Better FRET efficiency with Cy7® and Alexa Fluor® 750 than other commercial quenchers
Cat# 2105 (TQ7WS acid)
Cat# 2106 (TQ7WS amine)
Cat# 2109 (TQ7WS maleimide, SH-reactive)
Cat# 2111 (TQ7WS SE, NH2-reactive)
Cat# 2112 (TQ7WS azide, click chemistry)
Cat# 2113 (TQ7WS alkyne, click chemistry)
  1. BHQ is the is the trademark of Biosearch Technologies, Inc. Texas Red®, QSY® and Alexa Fluor® are the trademarks of Molecular Probes. CyDye, Cy3®, Cy5®, Cy5.5® and Cy7® are the trademarks of GE Health Care. DyLight™ is the trademark of ThermoFisher Corp. IRDye® 700 and IRDye® 800 are the trademarks of Li-COR. Tide Quencher™ and Tide Quencher™ are the trademarks of AAT Bioquest.

 

Chemical Properties of Tide Quencher™ Non-Fluorescent Labeling Dyes


AAT Bioquest offers a full set of Tide Quencher™ dyes that span the full visible spectrum. Tide Quencher™ dyes have improved labeling performance than the classic quencher dyes such as Dabcyl, Black Hole Quencher® and QSY® dyes. They can be readily used to develop various FRET probes for molecular diagnostics, proteomics and other biological applications.
 

Table 2. Chemical Properties of Tide Quencher™ Dyes

Cat No.
Labeling Dye
Reactivity
Adduct MW Calculation*
2188Tide Quencher™ 1 azide [TQ1 azide]Alkyne+ 358
2189Tide Quencher™ 1 alkyne [TQ1 alkyne]Azide+ 327
2190Tide Quencher™ 1 acid [TQ1 acid]NH2 and OH+ 272
2192Tide Quencher™ 1 amine [TQ1 amine]CO2H+ 314
2193, 2194Tide Quencher™ 1 CPG [TQ1 CPG]Oligo Synthesis+ 345
2196Tide Quencher™ 1 maleimide [TQ1 maleimide]SH+ 455
2199Tide Quencher™ 1 succinimidyl ester [TQ1 SE]Aliphatic amine+ 272
2200Tide Quencher™ 2 acid [TQ2 acid]NH2 and OH+ 364
2202Tide Quencher™ 2 amine [TQ2 amine]CO2H+ 406
2203, 2204Tide Quencher™ 1 CPG [TQ1 CPG]Oligo Synthesis+ 437
2210Tide Quencher™ 2 succinimidyl ester [TQ2 SE]Aliphatic amine+ 364
2211Tide Quencher™ 2 azide [TQ2 azide]Alkyne+ 450
2212Tide Quencher™ 2 alkyne [TQ2 alkyne]Azide+ 419
2050Tide Quencher™ 2WS acid [TQ2WS acid]NH2 and OH+ 472
2058Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]Aliphatic amine+ 472
2059Tide Quencher™ 2WS maleimide [TQ2WS maleimide]SH+ 612
2220Tide Quencher™ 3 acid [TQ3 acid]NH2 and OH+ 435
2222Tide Quencher™ 3 amine [TQ3 amine]CO2H+ 477
2223, 2224Tide Quencher™ 3 CPG [TQ3 CPG]Oligo Synthesis+ 508
2226Tide Quencher™ 3 maleimide [TQ3 maleimide]SH+ 575
2230Tide Quencher™ 3 succinimidyl ester [TQ3 SE]Aliphatic amine+ 435
2231Tide Quencher™ 3 azide [TQ3 azide]Alkyne+ 521
2232Tide Quencher™ 3 alkyne [TQ3 alkyne]Azide+ 490
2227NH2 and OHAzide+ 779
2229Aliphatic amineAzide+ 779
2062, 2063Tide Quencher™ 4 CPG [TQ4 CPG]Oligo Synthesis+ 501
2060Tide Quencher™ 4WS acid [TQ4WS acid]NH2 and OH+ 781
2061Tide Quencher™ 4WS amine [TQ4WS amine]CO2H+ 823
2064Tide Quencher™ 4WS maleimide [TQ4WS maleimide]SH+ 992
2067Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]Aliphatic amine+ 781
2068Tide Quencher™ 4WS azide [TQ4WS azide]Alkyne+ 887
2069Tide Quencher™ 4WS alkyne [TQ4WS alkyne]Azide+ 836
2077 , 2078Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å*Oligo Synthesis+ 569
2075Tide Quencher™ 5WS acid [TQ5WS acid]NH2 and OH 758
2076 Tide Quencher™ 5WS amine [TQ5WS amine]CO2H 799
2079 Tide Quencher™ 5WS maleimide [TQ5WS maleimide]SH 968
2081Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE]Aliphatic amine 758
2082Tide Quencher™ 5WS azide [TQ5WS azide]Alkyne 843
2083Tide Quencher™ 5WS alkyne [TQ5WS alkyne]Azide 812
2090Tide Quencher™ 6WS acid [TQ6WS acid]NH2 and OH 806
2091Tide Quencher™ 6WS amine [TQ6WS amine]CO2H 848
2094Tide Quencher™ 6WS maleimide [TQ6WS maleimide]SH1017
2096Tide Quencher™ 6WS succinimidyl ester [TQ6WS SE]Aliphatic amine806
2097Tide Quencher™ 6WS azide [TQ6WS azide]Alkyne892
2098Tide Quencher™ 6WS alkyne [TQ6WS alkyne]Azide861
2105Tide Quencher™ 7WS acid [TQ7WS acid]NH2 and OH783
2106Tide Quencher™ 7WS amine [TQ7WS amine]CO2H825
2109Tide Quencher™ 7WS maleimide [TQ7WS maleimide]SH994
2111Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE]Aliphatic amine783
2112Tide Quencher™ 7WS azide [TQ7WS azide]Alkyne869
2113Tide Quencher™ 7WS alkyne [TQ7WS alkyne]Azide838
  1. * The molecular weight of the desired conjugate = the molecular weight of the free unlabeled molecule + the value listed in the table.

 

Spectral Properties of Tide Quencher™ Non-Fluorescent Labeling Dyes


Tide Quencher™ dyes are a series of excellent labeling quenchers that span the full visible spectrum. Tide Quencher™ dyes have improved labeling performance than the classic quencher dyes such as Dabcyl, Black Hole Quencher® and QSY® dyes. They are the best affordable dyes for labeling oligos and peptides without comprised performance. Each Tide Quencher™ dye is developed to match the spectral properties of a particular fluorescent donor, e.g., Alexa Fluor® or other labeling dyes (such as DyLight™ dyes).
 

Table 3. Tide Quencher™ Dyes Spectral Properties

Quencher
Abs (nm)
ε¹
CF at 260 nm²
CF at 280 nm³
Molecular Weight Calculation (SE Format)⁴
Tide Quencher™ 149220,0000.1470.194+272
Tide Quencher™ 251621,0000.1000.120+364
Tide Quencher™ 2WS54148,0001.2960.559+472
Tide Quencher™ 357322,0000.0850.091+435
Tide Quencher™ 3WS57990,0000.1860.205+779
Tide Quencher™ 460323,0000.1460.183+501 (CPG format)
Tide Quencher™ 4WS60490,0000.1490.136+781
Tide Quencher™ 5WS661130,0000.0720.082+758
Tide Quencher™ 6WS694130,0000.1200.102+806
Tide Quencher™ 7WS764140,0000.0720.091+783
  1. Extinction Coefficient at their maximum absorption wavelength
  2. CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligo and nucleic acid labeling)
  3. CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptide and protein labeling).


Original created on January 3, 2020, last updated on January 3, 2020
Tagged under: Fluorescence Resonance Energy Transfer (FRET), Oligonucleotide Labeling, Peptide Labeling