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Are Annexin V-FITC+/PI+ cells classified as late-apoptotic cells or necrotic cells?
Posted May 9, 2020
Chemical ReagentsAV-FITCAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesPropidium iodide (PI)annexin V
Answer

Cells that stain Annexin V-FITC+/PI+ can be either late-apoptotic or necrotic.

Annexin V-FITC/PI staining can be used to distinguish late apoptotic and necrotic cells from early apoptotic cells, which stain Annexin V-FITC+/PI-. However, since both late apoptotic cells and necrotic cells stain Annexin V-FITC+/PI+, the two cannot be distinguished using this method.

Additional resources
Brauchle, E., Thude, S., Brucker, S. Y., & Schenke-Layland, K. (2014). Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy. Scientific reports, 4, 4698. Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*Annexin V, FITC Labeled

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