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Can Terminal Transferase (TdT) label both the 3' and 5' end of DNA?
Posted May 13, 2019
Does TdT have a preference for a specific type of 3' DNA end?
TUNELapoptosisnecrosisdNTPTerminal Transferase (TdT)
Answer
Terminal deoxynucleotidyl transferase (TdT) can only label the 3' end of DNA.
Addition of dNTPs to 3' OH protruding ends is more efficient than with 3' OH recessed or blunt ends.
Additional resources
Cell Meter™ TUNEL Apoptosis Assay KitTerminal deoxynucleotidyl transferase

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