AAT Bioquest

What filters should I use for Apoptosis/Necrosis Assay Kit (Cat No. 22840)?

Posted July 28, 2020


The Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence* (Cat No. 22840) requires three filter sets for visualization. See Table 1 and 2 below.

Table 1. Flow cytometer channels required for Cat No. 22840.

ProbeExcitation Laser (nm)ChannelChannel Specification (nm)Cell Population
CytoCalcein™ Violet 450405Pacific Blue450/40Viable Cells
Apopxin™ Green488FITC530/30Apoptotic Cells
7-AAD488PE-Cy5670/14Necrotic Cells

Table 2. Fluorescence microscope filter sets required for Cat No. 22840.

ProbeFilter SetFilter Specification (nm)Cell Population
CytoCalcein™ Violet 450DAPI440/40Viable Cells
Apopxin™ GreenFITC530/30Apoptotic Cells
7-AADTexas Red610/40Necrotic Cells
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