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What filters should I use for Apoptosis/Necrosis Assay Kit (Cat No. 22840)?

Posted July 28, 2020


Answer

The Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence* (Cat No. 22840) requires three filter sets for visualization. See Table 1 and 2 below.


Table 1. Flow cytometer channels required for Cat No. 22840.

Probe Excitation Laser (nm) Channel Channel Specification (nm) Cell Population
CytoCalcein™ Violet 450 405 Pacific Blue 450/40 Viable Cells
Apopxin™ Green 488 FITC 530/30 Apoptotic Cells
7-AAD 488 PE-Cy5 670/14 Necrotic Cells


Table 2. Fluorescence microscope filter sets required for Cat No. 22840.

Probe Filter Set Filter Specification (nm) Cell Population
CytoCalcein™ Violet 450 DAPI 440/40 Viable Cells
Apopxin™ Green FITC 530/30 Apoptotic Cells
7-AAD Texas Red 610/40 Necrotic Cells
Additional resources

https://www.aatbio.com/products/cell-meter-apoptotic-and-necrotic-multiplexing-detection-kit-i-triple-fluorescence-colors?unit=22840