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What filters should I use for Apoptosis/Necrosis Assay Kit (Cat No. 22840)?
Posted July 28, 2020
Answer
The Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence* (Cat No. 22840) requires three filter sets for visualization. See Table 1 and 2 below.
Table 1. Flow cytometer channels required for Cat No. 22840.
| Probe | Excitation Laser (nm) | Channel | Channel Specification (nm) | Cell Population |
| CytoCalcein™ Violet 450 | 405 | Pacific Blue | 450/40 | Viable Cells |
| Apopxin™ Green | 488 | FITC | 530/30 | Apoptotic Cells |
| 7-AAD | 488 | PE-Cy5 | 670/14 | Necrotic Cells |
Table 2. Fluorescence microscope filter sets required for Cat No. 22840.
| Probe | Filter Set | Filter Specification (nm) | Cell Population |
| CytoCalcein™ Violet 450 | DAPI | 440/40 | Viable Cells |
| Apopxin™ Green | FITC | 530/30 | Apoptotic Cells |
| 7-AAD | Texas Red | 610/40 | Necrotic Cells |
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