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Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
Posted June 12, 2019
ActinPhalloidinF-actin
Answer

Yes, it is ok to fix cells with glutaraldehyde.

Keep in mind, the fixation method should be selected based-on experimental conditions. Fixing cells with formaldehyde, paraformaldehyde or glutaraldehyde will result in good actin filament staining using phalloidin conjugates such as Phalloidin-iFluor® 488.

 
Additional resources
List of Fluorescent Phalloidin

How does phalloidin affect actin assembly?What is the difference between formaldehyde and paraformaldehyde in cell fixation?Why is phalloidin used in experiments that study actin?Before staining actin with phalloidin dye conjugates, how should I fixate my sample?Do I need to deparaffinize my paraffin-embedded tissue sections before staining F-actin with Phalloidin-iFluor® conjugates? Can you provide a deparaffinization protocol?