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AAT Bioquest

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Posted October 30, 2019


Answer
1. Could you please tell the customer what is the difference between the molecular structure of iFluor® 488 and Alexa Fluor™ 488? 
The spectral properties are similar to Alexa Fluor® 488 NHS ester.
The brightness is similar to  Alexa Fluor® 488 NHS ester.
Image intensity is almost the same or a little higher than AF488 conjugate.
The purity is ~95%, much better than AF488.
 
2. Could you please tell the customer if cat#1023 can be used to Label VHH?
Yes. It's no problem.
 
3. Could you please tell the customer if  iFluor® 488 is easy to quench?
It's not easy to quench. The Best Dye to antibody range is 4-8.