What are the spectral properties of iFluor® dyes?

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Accepted Answer

Table 1. Spectral properties of iFluor® fluorescent labeling dyes.

Dye

Absmax (nm)

Emmax (nm)

&epsilon; 1

&Phi; 2

CF at 260 nm 3

CF at 280 nm4

iFluor® 350

344

448

20000

0.95

0.83

0.23

iFluor® 405

402

425

37000

0.91

0.48

0.77

iFluor® 430

433

495

40000

0.78

0.68

0.3

iFluor® 450

451
502

40000

0.82

0.45

0.27

iFluor® 488

491

516

75000

0.9

0.21

0.11

iFluor® 514

527

554

80000

0.83

0.25

0.11

iFluor® 532

543

563

90000

0.68

0.26

0.16

iFluor® 546

541
557

100000

0.67

0.25

0.15

iFluor® 555

556
569

100000

0.64

0.23

0.14

iFluor® 560
559
571

130000

0.57

0.048

0.069

iFluor® 568

568

587

100000

0.57

0.34

0.15

iFluor® 594

587
603

180000

0.53

0.05

0.04

iFluor® 610

609
627

110000

0.85

0.32

0.49

iFluor® 633

638

652

250000

0.29

0.062

0.044

iFluor® 647

654
669

250000

0.25

0.03

iFluor® 660

660
677

250000

0.26

0.07

0.08

iFluor® 670

669
682

250000

0.63

0.019

0.024

iFluor® 680

683
700

220000

0.23

0.097

0.094

iFluor® 700

690
713

220000

0.23

0.09

0.04

iFluor® 710

712

736

190000

0.14

0.12

0.07

iFluor® 750

756
777

275000

0.12

0.044

0.039

iFluor® 790

786

811

250000

0.13

0.1

0.09

iFluor® 800

801

820

250000

0.11

0.03

0.08

iFluor® 810

811
822

250000

0.05

0.09

0.15

iFluor® 820

820

849

250000

N/D5

0.11

0.16

iFluor® 840

836

876

200000

0.02

0.2

0.09

iFluor® 860

852

877

250000

N/D5

0.1

0.14

Note:

Extinction coefficient at their maximum absorption wavelength. The units of extinction coefficient are cm-1M-1.
FQY = fluorescence quantum yield in aqueous buffer (pH 7.2).
CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm.
CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).
Fluorescence intensity is significantly increased upon coupling to proteins.

Dye	Abs_max(nm)	Em_max(nm)	ε ¹	Φ ²	CF at 260 nm ³	CF at 280 nm⁴
iFluor® 350	344	448	20000	0.95	0.83	0.23
iFluor® 405	402	425	37000	0.91	0.48	0.77
iFluor® 430	433	495	40000	0.78	0.68	0.3
iFluor® 450	451	502	40000	0.82	0.45	0.27
iFluor® 488	491	516	75000	0.9	0.21	0.11
iFluor® 514	527	554	80000	0.83	0.25	0.11
iFluor® 532	543	563	90000	0.68	0.26	0.16
iFluor® 546	541	557	100000	0.67	0.25	0.15
iFluor® 555	556	569	100000	0.64	0.23	0.14
iFluor® 560	559	571	130000	0.57	0.048	0.069
iFluor® 568	568	587	100000	0.57	0.34	0.15
iFluor® 594	587	603	180000	0.53	0.05	0.04
iFluor® 610	609	627	110000	0.85	0.32	0.49
iFluor® 633	638	652	250000	0.29	0.062	0.044
iFluor® 647	654	669	250000	0.25	0.03	0.03
iFluor® 660	660	677	250000	0.26	0.07	0.08
iFluor® 670	669	682	250000	0.63	0.019	0.024
iFluor® 680	683	700	220000	0.23	0.097	0.094
iFluor® 700	690	713	220000	0.23	0.09	0.04
iFluor® 710	712	736	190000	0.14	0.12	0.07
iFluor® 750	756	777	275000	0.12	0.044	0.039
iFluor® 790	786	811	250000	0.13	0.1	0.09
iFluor® 800	801	820	250000	0.11	0.03	0.08
iFluor® 810	811	822	250000	0.05	0.09	0.15
iFluor® 820	820	849	250000	N/D⁵	0.11	0.16
iFluor® 840	836	876	200000	0.02	0.2	0.09
iFluor® 860	852	877	250000	N/D⁵	0.1	0.14

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