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What are the spectral properties of iFluor dyes?
Posted August 20, 2019
Answer
Table 1. Spectral properties of iFluor® fluorescent labeling dyes.
|
Dye
|
Absmax (nm)
|
Emmax (nm)
|
ε 1
|
Φ 2
|
CF at 260 nm 3
|
CF at 280 nm4
|
| 344 |
448
|
20000
|
0.95
|
0.83
|
0.23
| |
|
402
| 425 |
37000
|
0.91
|
0.48
|
0.77
| |
|
433
| 495 |
40000
|
0.78
|
0.68
|
0.3
| |
| 451 | 502 |
40000
|
0.82
|
0.45
|
0.27
| |
| 491 |
516
|
75000
|
0.9
|
0.21
|
0.11
| |
|
527
| 554 |
80000
|
0.83
|
0.25
|
0.11
| |
|
543
| 563 |
90000
|
0.68
|
0.26
|
0.16
| |
| 541 | 557 |
100000
|
0.67
|
0.25
|
0.15
| |
| 556 | 569 |
100000
|
0.64
|
0.23
|
0.14
| |
| iFluor® 560 | 559 | 571 |
130000
|
0.57
|
0.048
|
0.069
|
| 568 |
587
|
100000
|
0.57
|
0.34
|
0.15
| |
| 587 | 603 |
180000
|
0.53
|
0.05
|
0.04
| |
| 609 | 627 |
110000
|
0.85
|
0.32
|
0.49
| |
|
638
| 652 |
250000
|
0.29
|
0.062
|
0.044
| |
| 654 | 669 |
250000
|
0.25
|
0.03
|
0.03
| |
| 660 | 677 |
250000
|
0.26
|
0.07
|
0.08
| |
| 669 | 682 |
250000
|
0.63
|
0.019
|
0.024
| |
| 683 | 700 |
220000
|
0.23
|
0.097
|
0.094
| |
| 690 | 713 |
220000
|
0.23
|
0.09
|
0.04
| |
|
712
| 736 |
190000
|
0.14
|
0.12
|
0.07
| |
| 756 | 777 |
275000
|
0.12
|
0.044
|
0.039
| |
|
786
| 811 |
250000
|
0.13
|
0.1
|
0.09
| |
|
801
| 820 |
250000
|
0.11
|
0.03
|
0.08
| |
| 811 | 822 |
250000
|
0.05
|
0.09
|
0.15
| |
|
820
|
849
|
250000
|
N/D5
|
0.11
|
0.16
| |
|
836
|
876
|
200000
| 0.02 |
0.2
|
0.09
| |
| 852 |
877
|
250000
| N/D5 |
0.1
|
0.14
|
Note:
- Extinction coefficient at their maximum absorption wavelength. The units of extinction coefficient are cm-1M-1.
- FQY = fluorescence quantum yield in aqueous buffer (pH 7.2).
- CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm.
- CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).
- Fluorescence intensity is significantly increased upon coupling to proteins.
Additional resources
Protocol for Labeling IgG with iFluor™ 488 succinimidyl ester