Laser line | Common fluorophores used with laser | Application |
Ultraviolet (355 nm) | iFluor® 350, XFD350 | protein/antibody labeling dye |
DAPI, Hoechst, Nuclear Blue™ DCS1 | DNA stain | |
Calcein Blue, Calcein UltraBlue™ | cell viability probe | |
Annexin V-iFluor® 350 conjugate | early-stage apoptosis probe | |
Violet (405 - 407 nm) | PacBlue, iFluor® 405, iFluor® 430, mFluor™ Violet 450, mFluor™ Violet 510, mFluor™ Violet 540 | protein/antibody labeling dye |
Nuclear Violet™ LCS1 | DNA stain | |
CytoCalcein™ Violet 450, CytoCalcien™ Violet 500 | cell viability indicator | |
CytoTell™ Blue, CytoTell™ Violet 500 | cell proliferation indicator | |
Annexin V-mFluor™ Violet 450 | early-stage apoptosis indicator | |
Blue (488 nm) | FITC, iFluor® 488, XFD488, PE, PE tandems, PerCP | protein/antibody labeling dye |
Nuclear Green™ LCS1, PI, 7-AAD | DNA stain | |
Calcein UltraGreen™ | cell viability indicator | |
CytoTell™ Green, CytoTell™ UltraGreen | cell proliferation indicator | |
Annexin V-iFluor® 488 | early-stage apoptosis indicator | |
Green (532 nm) | iFluor® 532, mFluor™ Blue 570, XFD532, PE, PE tandems | protein/antibody labeling dye |
Nuclear Orange™ LCS1, PI, 7-AAD | DNA stain | |
Calcein™ Orange | cell viability indicator | |
CytoTell™ Orange | cell proliferation indicator | |
Annexin V-mFluor™ Blue 570 | early-stage apoptosis indicator | |
Yellow (561 - 568 nm) | iFluor® 555, XFD555, iFluor® 568, PE, PE tandems | protein/antibody labeling dye |
PI, 7-AAD | DNA stain | |
Calcein Red™ | cell viability indicator | |
CytoTell™ Red 590 | cell proliferation indicator | |
Annexin V-iFluor® 555 | early-stage apoptosis indicator | |
Red (633 - 647 nm) | iFluor® 633, iFluor® 647, XFD647, APC, APC tandems | protein/antibody labeling dye |
Nuclear Red™ LCS1 | DNA stain | |
Calcein Deep Red™ | cell viability indicator | |
CytoTell™ Red 650 | cell proliferation indicator | |
Annexin V-iFluor® 633, Annexin V-iFluor® 647 | early-stage apoptosis indicator |