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What are common laser lines used in flow cytometry?
Posted August 21, 2019
Answer
Flow cytometers are commonly equipped with one or more of the following laser lines:
- Ultraviolet (355 nm)
- Violet (405-407 nm)
- Blue (488 nm)
- Green (532 nm)
- Yellow (561-568 nm)
- Red (633-647 nm)
Refer to Table 1 below for common fluorophores used with each laser line and their respective applications.
Table 1. Common laser lines used in flow cytometry.
| Laser line | Common fluorophores used with laser | Application |
|
Ultraviolet
(355 nm)
| iFluor® 350, Alpha Fluor™ 350 | protein/antibody labeling dye |
| DAPI, Hoechst, Nuclear Blue™ DCS1 | DNA stain | |
| Calcein Blue, Calcein UltraBlue™ | cell viability probe | |
| Annexin V-iFluor® 350 conjugate | early-stage apoptosis probe | |
|
Violet
(405 - 407 nm)
| PacBlue, iFluor® 405, iFluor® 430, mFluor™ Violet 450, mFluor™ Violet 510, mFluor™ Violet 540 | protein/antibody labeling dye |
| Nuclear Violet™ LCS1 | DNA stain | |
| CytoCalcein™ Violet 450, CytoCalcien™ Violet 500 | cell viability indicator | |
| CytoTell™ Blue, CytoTell™ Violet 500 | cell proliferation indicator | |
| Annexin V-mFluor™ Violet 450 | early-stage apoptosis indicator | |
|
Blue
(488 nm)
| FITC, iFluor® 488, Alpha Fluor™ 488, PE, PE tandems, PerCP | protein/antibody labeling dye |
| Nuclear Green™ LCS1, PI, 7-AAD | DNA stain | |
| Calcein UltraGreen™ | cell viability indicator | |
| CytoTell™ Green, CytoTell™ UltraGreen | cell proliferation indicator | |
| Annexin V-iFluor® 488 | early-stage apoptosis indicator | |
|
Green
(532 nm)
| iFluor® 532, mFluor™ Blue 570, Alpha Fluor™ 532, PE, PE tandems | protein/antibody labeling dye |
| Nuclear Orange™ LCS1, PI, 7-AAD | DNA stain | |
| Calcein™ Orange | cell viability indicator | |
| CytoTell™ Orange | cell proliferation indicator | |
| Annexin V-mFluor™ Blue 570 | early-stage apoptosis indicator | |
|
Yellow
(561 - 568 nm)
| iFluor® 555, AF555, iFluor® 568, PE, PE tandems | protein/antibody labeling dye |
| PI, 7-AAD | DNA stain | |
| Calcein Red™ | cell viability indicator | |
| CytoTell™ Red 590 | cell proliferation indicator | |
| Annexin V-iFluor® 555 | early-stage apoptosis indicator | |
|
Red
(633 - 647 nm)
| iFluor® 633, iFluor® 647, AF647, APC, APC tandems | protein/antibody labeling dye |
| Nuclear Red™ LCS1 | DNA stain | |
| Calcein Deep Red™ | cell viability indicator | |
| CytoTell™ Red 650 | cell proliferation indicator | |
| Annexin V-iFluor® 633, Annexin V-iFluor® 647 | early-stage apoptosis indicator |
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