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What are common laser lines used in flow cytometry?

Posted August 21, 2019


Answer
Flow cytometers are commonly equipped with one or more of the following laser lines:
  • Ultraviolet (355 nm)
  • Violet (405-407 nm)
  • Blue (488 nm)
  • Green (532 nm)
  • Yellow (561-568 nm)
  • Red (633-647 nm)
Refer to Table 1 below for common fluorophores used with each laser line and their respective applications.
 
Table 1. Common laser lines used in flow cytometry.
 
Laser line Common fluorophores used with laser Application
Ultraviolet
(355 nm)
iFluor® 350, Alpha Fluor™ 350 protein/antibody labeling dye
DAPI, Hoechst, Nuclear Blue™ DCS1 DNA stain
Calcein Blue, Calcein UltraBlue™ cell viability probe
Annexin V-iFluor® 350 conjugate early-stage apoptosis probe
Violet
(405 - 407 nm)
PacBlue, iFluor® 405, iFluor® 430, mFluor™ Violet 450, mFluor™ Violet 510, mFluor™ Violet 540 protein/antibody labeling dye
Nuclear Violet™ LCS1 DNA stain
CytoCalcein™ Violet 450, CytoCalcien™ Violet 500 cell viability indicator
CytoTell™ BlueCytoTell™ Violet 500 cell proliferation indicator 
Annexin V-mFluor™ Violet 450 early-stage apoptosis indicator
Blue
(488 nm)
FITC, iFluor® 488Alpha Fluor™ 488PE, PE tandems, PerCP protein/antibody labeling dye
Nuclear Green™ LCS1PI, 7-AAD DNA stain
Calcein UltraGreen™ cell viability indicator
CytoTell™ Green, CytoTell™ UltraGreen cell proliferation indicator
Annexin V-iFluor® 488 early-stage apoptosis indicator
Green
(532 nm)
 
iFluor® 532, mFluor™ Blue 570, Alpha Fluor™ 532PE, PE tandems protein/antibody labeling dye
Nuclear Orange™ LCS1, PI, 7-AAD DNA stain
Calcein™ Orange cell viability indicator
CytoTell™ Orange cell proliferation indicator
Annexin V-mFluor™ Blue 570 early-stage apoptosis indicator
Yellow
(561 - 568 nm)
 
iFluor® 555, AF555iFluor® 568PE, PE tandems protein/antibody labeling dye
PI, 7-AAD DNA stain
Calcein Red™ cell viability indicator
CytoTell™ Red 590 cell proliferation indicator
Annexin V-iFluor® 555 early-stage apoptosis indicator
Red
(633 - 647 nm)
 
iFluor® 633iFluor® 647, AF647APC, APC tandems protein/antibody labeling dye
Nuclear Red™ LCS1 DNA stain 
Calcein Deep Red™ cell viability indicator
CytoTell™ Red 650 cell proliferation indicator
Annexin V-iFluor® 633, Annexin V-iFluor® 647 early-stage apoptosis indicator
Additional resources

Flow Cytometry

PE and APC

Cell Meter™ Annexin V Binding Apoptosis Assay Kits

Annexin V Staining