Calcein AM | AAT Bioquest

Calcein Red™ AM

Calcein AM is one of the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications. For example, it is impossible to use Calcein AM in combination of GFP-tranfacted cells due to the same color to GFP. To address this color limitation of Calcein AM, we have developed Calcein Orange™, Calcein Red™ and Calcein Deep Red™. These new Calcein AM analogs enable the multicolor labeling and functional analysis of live cells in combination with Calcein AM. Non-fluorescent Calcein Red™ AM can easily get into live cells and hydrolyzes to generate strongly fluorescent Calcein Red™ dye. Calcein Red™ dye can be monitored with the common TRITC/Cy3 filter set. AAT Bioquest offers Calcein Red™ as a reference dye to Calcein Red™ AM.

Images of Live HeLa cells stained with Calcein Red <sup>TM</sup>, AM (Cat.21900). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21900	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Calcein Red™ AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.446 µL	492.228 µL	984.455 µL	4.922 mL	9.845 mL
5 mM	19.689 µL	98.446 µL	196.891 µL	984.455 µL	1.969 mL
10 mM	9.845 µL	49.223 µL	98.446 µL	492.228 µL	984.455 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1015.79 g/mol	=	mL	x	mM	=	mmol

Citations

View all 32 citations: Citation Explorer

Titrating chimeric antigen receptors on CAR T cells enabled by a microfluidic-based dosage-controlled intracellular mRNA delivery platform

Authors:

Chen, Yu-Hsi and Mirza, Mahnoor and Jiang, Ruoyu and Lee, Abraham P

Journal:

Biomicrofluidics (2024)

Fabrication of endothelialized capillary-like microchannel networks using sacrificial thermoresponsive microfibers

Authors:

Rector, John A and McBride, Lucas and Weber, Callie and Grossman, Kira and Sorets, Alexander and Ventura-Antunes, Lissa and Holtz, Isabella Ko and Young, Katherine and Schrag, Matthew and Lippmann, Ethan S and others,

Journal:

Biofabrication (2024)

Titering of Chimeric Antigen Receptors on CAR T Cells enabled by a Microfluidic-based Dosage-Controlled Intracellular mRNA Delivery Platform

Authors:

Chen, Yu-Hsi and Jiang, Ruoyu and Lee, Abraham P

Journal:

bioRxiv (2023): 2023--03

High-Throughput Neurite Outgrowth Assay Using GFP-Labeled iPSC-Derived Neurons

Authors:

Zhang, Li and Li, Shuaizhang and Xia, Menghang

Journal:

Current Protocols (2022): e542

AMPA receptor and RNA processing gene dysregulation are early determinants of selective motor neuron vulnerability in a mouse model of amyotrophic lateral sclerosis

Authors:

Zanganeh, Pardis F and Barton, Samantha K and Lim, Katherine and Qian, Elizabeth L and Crombie, Duncan E and Bye, Christopher R and Turner, Bradley J

Journal:

Brain Communications (2022)

References

View all 84 references: Citation Explorer

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Authors:

Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang

Journal:

Biochemical and Biophysical Research Communications (2017)

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine

Authors:

Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.

Journal:

Biophys J. (2006)

A vaccination and challenge model using calcein marked fish

Authors:

Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.

Journal:

Fish Shellfish Immunol (2006): 20

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging

Authors:

Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.

Journal:

Cytometry A (2005): 78

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake

Authors:

Schoonen WG, Westerink WM, de Roos JA, Debiton E.

Journal:

Toxicol In Vitro (2005): 505

Physical properties

Molecular weight	1015.79
Solvent	DMSO

Spectral properties

Excitation (nm)	562
Emission (nm)	576

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	532/561 nm laser
Emission	585/40 nm filter

Fluorescence microscope
Excitation	TRITC filter set
Emission	TRITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	540
Emission	590
Cutoff	570
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Fax
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Page updated on January 23, 2025