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Calcein, AM
CAS 148504-34-1
Calcein AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity as compared to calcein. The acetomethoxy (AM) derivate of calcein (calcein AM) is widely used for labeling live cells as it can be transported through the cellular membrane into live cells. The AM ester groups mask the part of the molecule that chelates calcium. Upon transporting into live cells cellular esterases cut off the AM groups, the molecule binds to calcium within cell (resulting in acquiring strong green fluorescence), and gets trapped inside. As dead cells lack esterases, only live cells are marked. This feature makes it very useful for testing of cell viability and for short-term marking of cells. Compared with other live cell-labeling reagents (such as BCECF-AM and carboxy-fluorescein diacetate), calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not significantly affect cellular functions such as proliferation or chemotaxis of lymophocyte. In addition, viability assays using calcein are reliable and correlate well with the standard 51Cr-release assay.
Simultaneous imaging of live and apoptotic HeLa cells labeled using calcein AM (Cat No. 22002) and Annexin V-iFluor® 647 conjugate (Cat No. 20074).
Simultaneous imaging of live and apoptotic HeLa cells labeled using calcein AM (Cat No. 22002) and Annexin V-iFluor® 647 conjugate (Cat No. 20074).
CatalogSize
Price
Quantity
220021 mg
Price
 
Physical properties

Molecular weight994.86
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
81000
Excitation (nm)501
Emission (nm)521
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS148504-34-1
Instrument settings

Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 13, 2024