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CytoTell® UltraGreen
Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, there are a few severe problems associated with the use of CFSE for monitoring cell proliferation. 1). CFSE is highly toxic to cells. CFSE indiscriminately reacts with all amino groups, thus changes many critical intracellular protein functions (such as cell membrane GPCRs); 2). CFSE has slow response and is inconvenient to use. The CFSE fluorescence intensity of the 2nd generation cells is decreased more than 10 fold from the 1st generation. You would have to wait for another generation to start the cell proliferation analysis. 3). Medium removal is required. You would have to remove medium for cell analysis with a flow cytometer since CFSE reacts with medium components. CytoTell® Green has been developed to eliminate these CFSE limitations. Based on our customers' feedbacks on our CytoTell® Green, CytoTell® UltraGreen is our newest improvement. It has distinct advantages. 1). CytoTell® UltraGreen is well retained in cells; 2). CytoTell® UltraGreen exhibits much faster response and is more convenient to use than CFSE. The fluorescence intensity gap between 1st and 2nd generation is significantly minimized. As cells divide, CytoTell® UltraGreen is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye; 3). CytoTell® UltraGreen is more sensitive than CFSE. Up to 9 generations may be visualized; 4). CytoTell® UltraGreen is much more stable than CFSE. CytoTell® UltraGreen stock solution can be stored at room temperature for a few days. CytoTell® UltraGreen can also be used for long term tracking of labeled cells. Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation. CytoTell® UltraGreen has a peak excitation of 492 nm and can be excited by the blue (488 nm) laser line, making it compatible with FITC filter set.
Cell tracking assay using CytoTell™ UltraGreen. Jurkat cells (~2x10^6 cells/mL) were stained with CytoTell™ UltraGreen on Day 0. Cells were passed serially at 1:1 ratio for 7 days. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel. Successive generations were represented by different colors.
Cell tracking assay using CytoTell™ UltraGreen. Jurkat cells (~2x10^6 cells/mL) were stained with CytoTell™ UltraGreen on Day 0. Cells were passed serially at 1:1 ratio for 7 days. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel. Successive generations were represented by different colors.
protocol
Protocol
COA
CatalogSize
Price
Quantity
22240500 Tests
Price
222412x500 Tests
Price
 
Physical properties
Molecular weight~500
SolventDMSO
Spectral properties
Excitation (nm)492
Emission (nm)514
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
Documents
Protocol
Safety Data Sheet (Catalog 22240)
Safety Data Sheet (Catalog 22241)
Certificate of Analysis
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Page updated on October 24, 2025