Name: CytoTell™ Blue
Brand: AAT Bioquest
SKU: 22251
Availability: InStock

CytoTell™ Blue

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for the applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC. CytoTell™ dyes are well excited at major laser lines such as 405 nm, 488 nm or 633 nm with multicolor emissions. CytoTell™ dyes have minimal cytotoxicity, and are used for the multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein. CytoTell™ Blue is a blue fluorescent dye that stains cells evenly. As cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye. Cells labeled with CytoTell™ Blue may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers. CytoTell™ Blue has a peak excitation of 405 nm and can be excited by the violet (405 nm) laser line. It has a peak emission of 450 nm and can be detected with a 450/20 band pass filter (equivalent to Pacific Blue®, or BD Horizon® V450), making it compatible with applications that utilize GFP or FITC antibodies for multicolor cell analysis.

Protocol

COA

Catalog	Size	Price	Quantity
22251	500 Tests	Price
22252	2x500 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTell™ Blue to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	263.359 µL	1.317 mL	2.634 mL	13.168 mL	26.336 mL
5 mM	52.672 µL	263.359 µL	526.718 µL	2.634 mL	5.267 mL
10 mM	26.336 µL	131.679 µL	263.359 µL	1.317 mL	2.634 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	379.71 g/mol	=	mL	x	mM	=	mmol

Citations

View all 21 citations: Citation Explorer

Impact of PD-1+ Tim-3+ CD8+ T cells in pretreatment tumors on chemotherapy responses in esophageal cancer

Authors:

Sumimoto, Tomoko and Yamamoto, Kei and Saito, Takuro and Urakawa, Shinya and Takeoka, Tomohira and Makino, Tomoki and Kurokawa, Yukinori and Kawashima, Atsunari and Ueyama, Azumi and Eguchi, Hidetoshi and others,

BCG immunization mitigates SARS-CoV-2 replication in macaques via monocyte efferocytosis and neutrophil recruitment in lungs

Authors:

Rahman, Mohammad Arif and Goldfarbmuren, Katherine C and Sarkis, Sarkis and Bissa, Massimiliano and Gutowska, Anna and Schifanella, Luca and Moles, Ramona and Doster, Melvin N and Andersen, Hanne and Jethmalani, Yogita and others,

Journal:

JCI insight (2025)

Sustained inhibition of CSF1R signaling augments antitumor immunity through inhibiting tumor-associated macrophages

Authors:

Sato, Takahiko and Sugiyama, Daisuke and Koseki, Jun and Kojima, Yasuhiro and Hattori, Satomi and Sone, Kazuki and Nishinakamura, Hitomi and Ishikawa, Tomohiro and Ishikawa, Yuichi and Kato, Takuma and others,

Journal:

JCI insight (2025): e178146

Inhibition of furin in CAR macrophages directs them toward a proinflammatory phenotype and enhances their antitumor activities

Authors:

Ziane-Chaouche, Lydia and Raffo-Romero, Antonella and Hajjaji, Nawale and Kobeissy, Firas and Pinheiro, Donna and Aboulouard, Soulaimane and Cozzani, Adeline and Mitra, Suman and Fournier, Isabelle and Cizkova, Dasa and others,

Journal:

Cell Death \& Disease (2024): 1--16

The CH1 domain influences the expression and antigen sensing of the HIV-specific CH31 IgM-BCR and IgG-BCR

Authors:

Ortiz, Yaneth and Anasti, Kara and Pane, Advaiti K and Cronin, Kenneth and Alam, S Munir and Reth, Michael

Journal:

Proceedings of the National Academy of Sciences (2024): e2404728121

References

View all 50 references: Citation Explorer

Phenotyping of circulating CD8(+) T cell subsets in human cutaneous leishmaniasis

Authors:

Khamesipour A, Nateghi Rostami M, Tasbihi M, Miramin Mohammadi A, Shahrestani T, Sarrafnejad A, Sohrabi Y, Esk and ari SE, Keshavarz Valian H.

Journal:

Microbes Infect (2012): 702

Evaluation of multitype mathematical models for CFSE-labeling experiment data

Authors:

Miao H, Jin X, Perelson AS, Wu H.

Journal:

Bull Math Biol (2012): 300

Repetitive transplantation of different cell types sequentially improves heart function after infarction

Authors:

Alex, undefined and er S, Sasse A, Konschalla S, Kroh A, Merx MW, Weber C, Liehn EA.

Journal:

J Cell Mol Med (2012): 1640

Identification and isolation of slow-dividing cells in human glioblastoma using carboxy fluorescein succinimidyl ester (CFSE)

Authors:

Deleyrolle LP, Rohaus MR, Fortin JM, Reynolds BA, Azari H.

Journal:

J Vis Exp. (2012)

New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes

Authors:

Quah BJ, Parish CR.

Journal:

J Immunol Methods (2012): 1

Physical properties

Molecular weight	379.71
Solvent	DMSO

Spectral properties

Excitation (nm)	410
Emission (nm)	445

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	450/40 nm filter
Instrument specification(s)	Pacific Blue channel

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Page updated on October 13, 2025