CytoTell™ Blue
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 379.71 |
Solvent | DMSO |
Excitation (nm) | 410 |
Emission (nm) | 445 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
CytoTell™ Green |
CytoTell™ Orange |
CytoTell™ Red 650 |
CytoTell™ Red 590 |
CytoTell™ Violet 500 |
Overview | ![]() ![]() |
Molecular weight 379.71 | Excitation (nm) 410 | Emission (nm) 445 |
Platform
Flow cytometer
Excitation | 405 nm laser |
Emission | 450/40 nm filter |
Instrument specification(s) | Pacific Blue channel |
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells with test compounds
- Add 1X dye working solution
- Incubate dyes with cells at room temperature or 37 oC for 10 to 30 minutes
- Remove the dye working solution
- Analyse with flow cytometer with appropriate filter set
Important notes
Bring all the kit components at room temperature before starting the experiment. Note: The CytoTell™ dyes are lyophilized powders. They should be stable for at least 6 months if store at -20 °C, protecting from light, and avoiding freeze/thaw cycles.
Product Number |
Indicator |
Size |
Ex/Em (nm) |
Excitation Source |
22240 |
CytoTell™ UltraGreen |
500 tests |
492/519 |
488 nm (Blue Laser) |
22241 |
CytoTell™ UltraGreen |
1000 tests |
492/519 |
488 nm (Blue Laser) |
22248 |
CytoTell™ Violet 500 |
500 tests |
415/499 |
405 nm (Violet Laser) |
22251 |
CytoTell™ Blue |
500 tests |
403/454 |
405 nm (Violet Laser) |
22252 |
CytoTell™ Blue |
1000 tests |
403/454 |
405 nm (Violet Laser) |
22253 |
CytoTell™ Green |
500 tests |
511/525 |
488 nm (Blue Laser) |
22254 |
CytoTell™ Green |
1000 tests |
511/525 |
488 nm (Blue Laser) |
22255 |
CytoTell™ Red 650 |
500 tests |
628/643 |
633 nm (Red Laser) |
22256 |
CytoTell™ Red 650 |
1000 tests |
628/643 |
633 nm (Red Laser) |
22257 |
CytoTell™ Orange |
500 tests |
542 /556 |
488 nm (Blue Laser) |
22258 |
CytoTell™ Orange |
1000 tests |
542 /556 |
488 nm (Blue Laser) |
22261 |
CytoTell™ Red 590 |
500 tests |
560 /574 |
488 nm (Blue Laser) |
22262 |
CytoTell™ Red 590 |
1000 tests |
560 /574 |
488 nm (Blue Laser) |
PREPARATION OF STOCK SOLUTION
CytoTell™ dye stock solution (500X):
Add 500 µL DMSO into the dye powder vial, mix it well by vortexing to have a stock solution (500X). Note: The stock solution should be used promptly; any remaining solution should be aliquoted and frozen at < - 20 oC. Avoid repeated freeze-thaw cycles, and protect from light.
PREPARATION OF WORKING SOLUTION
CytoTellTM dye working solution (1X):
Dilute the 500X DMSO stock solution at 1 to 500 in Hanks and 20 mM Hepes buffer (HHBS) or the buffer of your choice, pH 7 (such as 1 µL of 500X DMSO stock solution to 500 µL buffer) right before use. Mix them well by vortexing. Note: The final concentration of the dye working solution should be empirically determined for different cell types and/or experimental conditions. It is recommended to test at the concentrations that are at least over ten fold range. Such as CytoTell™ Red might use much less amount in some cell types than the recommend concentrations.
SAMPLE EXPERIMENTAL PROTOCOL
- Treat cells with test compounds for a desired period of time.
- Centrifuge the cells to get 1-5 × 105 cells per tube.
- Resuspend cells in 500 µL of the CytoTell™ dye working solution. Optional: One can add the 500X DMSO stock solution into the cells directly without medium removing (such as, add 1 µL500X DMSO stock solution into 500 µL cells)
- Incubate cells with a dye solution at room temperature or 37 °C for 10 to 30 minutes, protected from light.
- Remove the dye working solution from the cells, wash the cells with HHBS or buffer of your choice. Resuspend cells in 500 µL of pre-warmed HHBS or medium to get 1-5 × 105 cells per tube.
- Monitor the fluorescence change at respected Ex/Em (see Table 1) with a flow cytometer or a fluorescence microscope.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 263.359 µL | 1.317 mL | 2.634 mL | 13.168 mL | 26.336 mL |
5 mM | 52.672 µL | 263.359 µL | 526.718 µL | 2.634 mL | 5.267 mL |
10 mM | 26.336 µL | 131.679 µL | 263.359 µL | 1.317 mL | 2.634 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Citations
Authors: Haruna, Miya and Ueyama, Azumi and Yamamoto, Yoko and Hirata, Michinari and Goto, Kumiko and Yoshida, Hiroshi and Higuchi, Naoko and Yoshida, Tetsuya and Kidani, Yujiro and Nakamura, Yamami and others,
Journal: Scientific Reports (2022): 1--12
Authors: Jin, Liyuan and Cao, Lei and Zhu, Yingjie and Cao, Jiani and Li, Xiaoyan and Zhou, Jianxia and Liu, Bing and Zhao, Tongbiao
Journal: Science Bulletin (2020)
Authors: Huang, Jianhan and Zheng, Meijun and Zhang, Zongliang and Tang, Xin and Chen, Yaxing and Tong, Aiping and Zhou, Liangxue
Journal: (2020)
Authors: Sawada, Masaaki and Goto, Kumiko and Morimoto-Okazawa, Akiko and Haruna, Miya and Yamamoto, Kei and Yamamoto, Yoko and Nakagawa, Satoshi and Hiramatsu, Kosuke and Matsuzaki, Shinya and Kobayashi, Eiji and others,
Journal: International Immunology (2020): 397--405
Authors: Rajasinghe, Lichchavi D and Chauhan, Preeti S and Wierenga, Kathryn A and Evered, Augustus O and Harris, Shamya N and Bates, Melissa A and Gavrilin, Mikhail A and Pestka, James J
Journal: Frontiers in immunology (2020): 2179
Authors: Nakagawa, Naoki and Plestant, Charlotte and Yabuno-Nakagawa, Keiko and Li, Jingjun and Lee, Janice and Huang, Chu-Wei and Lee, Amelia and Krupa, Oleh and Adhikari, Aditi and Thompson, Suriya and others,
Journal: Neuron (2019): 836--852
Authors: Nakajima, Masao and Sakoda, Yukimi and Adachi, Keishi and Nagano, Hiroaki and Tamada, Koji
Journal: Cancer science (2019): 3079--3088
Authors: Vitali, Ilaria and Fi{\`e}vre, Sabine and Telley, Ludovic and Oberst, Polina and Bariselli, Sebastiano and Frangeul, Laura and Baumann, Natalia and McMahon, John J and Klingler, Esther and Bocchi, Riccardo and others,
Journal: Cell (2018): 1264--1276
Authors: Govindan, Subashika and Oberst, Polina and Jabaudon, Denis
Journal: Nature protocols (2018): 2297--2311
Authors: Klöss, Volker
Journal: (2017)
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