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Nuclear Violet™ LCS1 *5 mM DMSO Solution*

Fluorescence image of live HeLa cells stained with Nuclear Violet™ LCS1(Cat. 17543) and MitoLite™ Deep Red FX660 (Cat. 22678) using fluorescence microscope with a DAPI and Cy5 filter set.
Fluorescence image of live HeLa cells stained with Nuclear Violet™ LCS1(Cat. 17543) and MitoLite™ Deep Red FX660 (Cat. 22678) using fluorescence microscope with a DAPI and Cy5 filter set.
Fluorescence image of live HeLa cells stained with Nuclear Violet™ LCS1(Cat. 17543) and MitoLite™ Deep Red FX660 (Cat. 22678) using fluorescence microscope with a DAPI and Cy5 filter set.
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Physical properties
Molecular weightN/A
Spectral properties
Excitation (nm)401
Emission (nm)460
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Our Nuclear Violet™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Violet™ LCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. It is well excited by violet laser at 405 nm, and emits blue/cyan fluorescence light around an emission maximum at ~460 nm, and provides an excellent tool for flow cytometers equipped with a 405 nm violet laser source. This DNA-binding dye might be used for multicolor analysis of live cells with the filter sets of Pacific Blue and BD Horizon V450.


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Spectral properties

Excitation (nm)401
Emission (nm)460



View all 9 citations: Citation Explorer
The Molecular Signature of Neuropathic Pain in a Human Model System
Authors: Sandy-Hindmarch, Oliver and Chang, Pao-Sheng and Scheuren, Paulina Simonne and De Schoenmacker, Iara and Hubli, Michele and Loizou, Constantinou and Wirth, Stephan and Mahadevan, Devendra and Wiberg, Akira and Furniss, Dominic and others,
Journal: medRxiv (2024): 2024--01
Activation of invasion by oncogenic reprogramming of cholesterol metabolism via increased NPC1 expression and macropinocytosis
Authors: Skorda, Aikaterini and Lauridsen, Anna R{\o}ssberg and Wu, Chengnan and Huang, Jinrong and Mrackova, Monika and Winther, Nuggi Ingholt and Jank, Vanessa and Sztupinszki, Zsofia and Strauss, Robert and Bilgin, Mesut and others,
Journal: Oncogene (2023): 1--12
Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections
Authors: Honda, Mizuki and Kimura, Ryuichi and Harada, Akihito and Maehara, Kazumitsu and Tanaka, Kaori and Ohkawa, Yasuyuki and Oki, Shinya
Journal: STAR protocols (2022): 101346
Real-time 3D high-resolution microscopy of human cells on the international space station
Authors: Thiel, Cora Sandra and Tauber, Svantje and Seebacher, Christian and Schropp, Martin and Uhl, Rainer and Lauber, Beatrice and Polzer, Jennifer and Neelam, Srujana and Zhang, Ye and Ullrich, Oliver
Journal: International journal of molecular sciences (2019): 2033
Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs
Authors: Roberts, Brock and Hendershott, Melissa C and Arakaki, Joy and Gerbin, Kaytlyn A and Malik, Haseeb and Nelson, Angelique and Gehring, Jamie and Hookway, Caroline and Ludmann, Susan A and Yang, Ruian and others, undefined
Journal: Stem cell reports (2019)
Discovery of Orally Bioavailable, Quinoline-based Aldehyde Dehydrogenase 1A1 (ALDH1A1) Inhibitors with Potent Cellular Activity
Authors: Yang, Shyh-Ming and Martinez, Natalia J and Yasgar, Adam and Danchik, Carina and Johansson, Catrine and Wang, Yuhong and Baljinnyam, Bolormaa and Wang, Amy Q and Xu, Xin and Shah, Pranav and others, undefined
Journal: Journal of medicinal chemistry (2018)
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1


View all 65 references: Citation Explorer
In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751
Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706
Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555
Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887
Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93
Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759
Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84
Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I
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Journal: Nat Protoc (2007): 269