AAT Bioquest

Does PBS buffer have any advantage over SSC buffer?

Posted April 17, 2020


In fluorescence in situ hybridization (FISH), it is beneficial to use SSC buffer since it helps to control for stringency of the hybridization. The stringency can be varied using different concentrations of SSC. Lower stringency allows hybridization of sequences that are not completely homologous, while higher stringency ensures hybridization of completely homologous sequences.

Additional resources

PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe